Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.)
Flax (<i>Linum usitatissimum</i> L.) is an important oilseed crop widely cultivated for its oil and fiber. This study conducted transcriptome analysis to analyze the gene expression profiles of roots, leaves, stamens, pistils, and fruits in the flax cultivar Longya10. A total of 43,471 g...
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3260 |
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| author | Fan Qi Fu Wang Chunxiao Xiaoyang Zhenhui Wang Yujie Lin Zhanwu Peng Jun Zhang Ningning Wang Jian Zhang |
| author_facet | Fan Qi Fu Wang Chunxiao Xiaoyang Zhenhui Wang Yujie Lin Zhanwu Peng Jun Zhang Ningning Wang Jian Zhang |
| author_sort | Fan Qi |
| collection | DOAJ |
| description | Flax (<i>Linum usitatissimum</i> L.) is an important oilseed crop widely cultivated for its oil and fiber. This study conducted transcriptome analysis to analyze the gene expression profiles of roots, leaves, stamens, pistils, and fruits in the flax cultivar Longya10. A total of 43,471 genes were detected in the RNA-seq data, with 34,497 genes showing differential expression levels between different organs. Gene expression patterns varied across different organs, with differences observed in expression-regulating genes within specific organs. However, 23,448 genes were found to be commonly expressed across all organs. Further analysis revealed organ-specific gene expressions, with 236, 690, 544, 909, and 1212 genes identified in pistils, fruits, leaves, roots, and stamens, respectively. Gene Ontology (GO) enrichment analysis was performed on these organ-specific genes, and significant enrichment was observed in various biological processes, cellular components, and molecular functions, providing new insights for the specific growth patterns of flax organs. Furthermore, we investigated the expression differences of AP2 transcription factors in various tissues and organs of Longya10. We identified 96 AP2 genes that were differentially expressed in different organs and annotated them into various biological pathways. Our results suggest that AP2 transcription factors may play important roles in regulating the growth and development of flax organs including stress response. In summary, our study provides a comprehensive analysis of gene expression patterns in different organs and tissues of flax plant and identifies potential critical regulators of flax organ growth and development. These findings contribute to a better understanding of the molecular mechanisms underlying flax organ development and may have important implications for the genetic improvement of flax crops. |
| first_indexed | 2024-03-10T22:13:50Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-10T22:13:50Z |
| publishDate | 2023-09-01 |
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| series | Plants |
| spelling | doaj.art-e6053cbff7814d309050154cb32072472023-11-19T12:32:20ZengMDPI AGPlants2223-77472023-09-011218326010.3390/plants12183260Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.)Fan Qi0Fu Wang1Chunxiao Xiaoyang2Zhenhui Wang3Yujie Lin4Zhanwu Peng5Jun Zhang6Ningning Wang7Jian Zhang8Faculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaInformation Center, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130000, ChinaFlax (<i>Linum usitatissimum</i> L.) is an important oilseed crop widely cultivated for its oil and fiber. This study conducted transcriptome analysis to analyze the gene expression profiles of roots, leaves, stamens, pistils, and fruits in the flax cultivar Longya10. A total of 43,471 genes were detected in the RNA-seq data, with 34,497 genes showing differential expression levels between different organs. Gene expression patterns varied across different organs, with differences observed in expression-regulating genes within specific organs. However, 23,448 genes were found to be commonly expressed across all organs. Further analysis revealed organ-specific gene expressions, with 236, 690, 544, 909, and 1212 genes identified in pistils, fruits, leaves, roots, and stamens, respectively. Gene Ontology (GO) enrichment analysis was performed on these organ-specific genes, and significant enrichment was observed in various biological processes, cellular components, and molecular functions, providing new insights for the specific growth patterns of flax organs. Furthermore, we investigated the expression differences of AP2 transcription factors in various tissues and organs of Longya10. We identified 96 AP2 genes that were differentially expressed in different organs and annotated them into various biological pathways. Our results suggest that AP2 transcription factors may play important roles in regulating the growth and development of flax organs including stress response. In summary, our study provides a comprehensive analysis of gene expression patterns in different organs and tissues of flax plant and identifies potential critical regulators of flax organ growth and development. These findings contribute to a better understanding of the molecular mechanisms underlying flax organ development and may have important implications for the genetic improvement of flax crops.https://www.mdpi.com/2223-7747/12/18/3260gene expressionorgantranscription factorAP2 |
| spellingShingle | Fan Qi Fu Wang Chunxiao Xiaoyang Zhenhui Wang Yujie Lin Zhanwu Peng Jun Zhang Ningning Wang Jian Zhang Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.) Plants gene expression organ transcription factor AP2 |
| title | Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.) |
| title_full | Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.) |
| title_fullStr | Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.) |
| title_full_unstemmed | Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.) |
| title_short | Gene Expression Analysis of Different Organs and Identification of AP2 Transcription Factors in Flax (<i>Linum usitatissimum</i> L.) |
| title_sort | gene expression analysis of different organs and identification of ap2 transcription factors in flax i linum usitatissimum i l |
| topic | gene expression organ transcription factor AP2 |
| url | https://www.mdpi.com/2223-7747/12/18/3260 |
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