Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection
Background & Objective: Diabetic foot ulcer (DFU), is one of the most frequent causes for hospitalizations in patients with diabetes. A major problem in the treatment of DFU is the increased-incidence of methicillin-resistant Staphylococcus aureus (MRSA). The aim of this study was to determine t...
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Iranian Society of Pathology
2019-10-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_36662_add6111a1d1590a50667ab6cf5340469.pdf |
| _version_ | 1819009455374729216 |
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| author | Pegah Kananizadeh Solmaz Ohadian Moghadam Yasaman Sadeghi Abbas Rahimi Foroushani Hossein Adibi Mohammad Reza Pourmand |
| author_facet | Pegah Kananizadeh Solmaz Ohadian Moghadam Yasaman Sadeghi Abbas Rahimi Foroushani Hossein Adibi Mohammad Reza Pourmand |
| author_sort | Pegah Kananizadeh |
| collection | DOAJ |
| description | Background & Objective: Diabetic foot ulcer (DFU), is one of the most frequent causes for hospitalizations in patients with diabetes. A major problem in the treatment of DFU is the increased-incidence of methicillin-resistant Staphylococcus aureus (MRSA). The aim of this study was to determine the SCCmec types of MRSA isolates and their epidemiology among patients with diabetes. Methods: This study was carried out on 145 diabetic patients with DFUs. The antibiotic susceptibility tests (ASTs) were performed using the disk diffusion method and E-test technique. SCCmec typing was done by multiplex PCR. Moreover, the presence of virulence toxin genes, including pvl and lukED was detected by PCR assay. Result: In 145 samples from which S. aureus was predominantly isolated, 19.48% were MRSA. Analysis of MRSA isolates revealed that the most prevalent SCCmec type was type IV (46.7%) followed by type III (30.0%) and type V (20.0%). One strain (3.3%) was untypeable. The prevalence of pvl and lukED was 56.7% and 100%, respectively. Conclusion: The high prevalence of MRSA in DFUs represents the high levels of antibiotic usage among patients with diabetes. In this study, resistance to other important clinical antibiotics was detected among MRSA isolates. The high proportion of SCCmec type IV and V strains, even in former hospitalized patients, indicates the entrance of these clones to the clinical setting. |
| first_indexed | 2024-12-21T00:56:38Z |
| format | Article |
| id | doaj.art-e60573324af8496597575d514405e998 |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-21T00:56:38Z |
| publishDate | 2019-10-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-e60573324af8496597575d514405e9982022-12-21T19:21:17ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562019-10-0114432933710.30699/ijp.2019.101092.203536662Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot InfectionPegah Kananizadeh0Solmaz Ohadian Moghadam1Yasaman Sadeghi2Abbas Rahimi Foroushani3Hossein Adibi4Mohammad Reza Pourmand5Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranUro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Science. Tehran, IranDiabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences. Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBackground & Objective: Diabetic foot ulcer (DFU), is one of the most frequent causes for hospitalizations in patients with diabetes. A major problem in the treatment of DFU is the increased-incidence of methicillin-resistant Staphylococcus aureus (MRSA). The aim of this study was to determine the SCCmec types of MRSA isolates and their epidemiology among patients with diabetes. Methods: This study was carried out on 145 diabetic patients with DFUs. The antibiotic susceptibility tests (ASTs) were performed using the disk diffusion method and E-test technique. SCCmec typing was done by multiplex PCR. Moreover, the presence of virulence toxin genes, including pvl and lukED was detected by PCR assay. Result: In 145 samples from which S. aureus was predominantly isolated, 19.48% were MRSA. Analysis of MRSA isolates revealed that the most prevalent SCCmec type was type IV (46.7%) followed by type III (30.0%) and type V (20.0%). One strain (3.3%) was untypeable. The prevalence of pvl and lukED was 56.7% and 100%, respectively. Conclusion: The high prevalence of MRSA in DFUs represents the high levels of antibiotic usage among patients with diabetes. In this study, resistance to other important clinical antibiotics was detected among MRSA isolates. The high proportion of SCCmec type IV and V strains, even in former hospitalized patients, indicates the entrance of these clones to the clinical setting.https://ijp.iranpath.org/article_36662_add6111a1d1590a50667ab6cf5340469.pdfdiabetic foot infectionmethicillin-resistant staphylococcus aureussccmec typing |
| spellingShingle | Pegah Kananizadeh Solmaz Ohadian Moghadam Yasaman Sadeghi Abbas Rahimi Foroushani Hossein Adibi Mohammad Reza Pourmand Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection Iranian Journal of Pathology diabetic foot infection methicillin-resistant staphylococcus aureus sccmec typing |
| title | Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection |
| title_full | Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection |
| title_fullStr | Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection |
| title_full_unstemmed | Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection |
| title_short | Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection |
| title_sort | molecular characteristics of methicillin resistant staphylococcus aureus mrsa isolated from diabetic foot infection |
| topic | diabetic foot infection methicillin-resistant staphylococcus aureus sccmec typing |
| url | https://ijp.iranpath.org/article_36662_add6111a1d1590a50667ab6cf5340469.pdf |
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