WRKY Genes Improve Drought Tolerance in Arachis duranensis
WRKY transcription factor participates in plant growth and development and response to biotic and abiotic stresses. Arachis duranensis, a turfgrass, has high drought tolerance, yet little is known about AdWRKYs response to drought stress in A. duranensis. In this study, RNA-seq identified five AdWRK...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.910408/full |
| _version_ | 1818209548018647040 |
|---|---|
| author | Yongli Zhang Pei Du Faqian Xiong Xiaojun Zhang Hui Song |
| author_facet | Yongli Zhang Pei Du Faqian Xiong Xiaojun Zhang Hui Song |
| author_sort | Yongli Zhang |
| collection | DOAJ |
| description | WRKY transcription factor participates in plant growth and development and response to biotic and abiotic stresses. Arachis duranensis, a turfgrass, has high drought tolerance, yet little is known about AdWRKYs response to drought stress in A. duranensis. In this study, RNA-seq identified five AdWRKYs, including AdWRKY18, AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, which were upregulated under drought stress. Orthologous relationships between AdWRKYs and Arabidopsis WRKY were determined to predict the regulatory networks of the five AdWRKYs based on AtWRKYs. Additionally, protein–protein interactions were predicted using differentially expressed proteins from RNA-seq. The quantitative real-time PCR (qRT-PCR) results showed that AdWRKY40 was upregulated, while AdWRKY42, AdWRKY56, and AdWRKY64 were downregulated at different time-points under drought stress. The predicted regulatory networks showed that AdWRKY40 activates COR47, RD21, and RD29A expression under drought stress. Besides, AdWRKY56 regulated CesA8 under drought stress. Aradu.YIQ80 (NAC019) interacted with AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, while Aradu.Z5H58 (NAC055) interacted with AdWRKY42 and AdWRKY64 under drought stress. This study used Arabidopsis to assess AdWRKYs function and regulatory networks, providing a basis for understanding drought tolerance in A. duranensis. |
| first_indexed | 2024-12-12T05:02:27Z |
| format | Article |
| id | doaj.art-e605c1302e404a8fa88b1301ffbe5ed3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T05:02:27Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e605c1302e404a8fa88b1301ffbe5ed32022-12-22T00:37:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.910408910408WRKY Genes Improve Drought Tolerance in Arachis duranensisYongli Zhang0Pei Du1Faqian Xiong2Xiaojun Zhang3Hui Song4Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, ChinaCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaGrassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, ChinaWRKY transcription factor participates in plant growth and development and response to biotic and abiotic stresses. Arachis duranensis, a turfgrass, has high drought tolerance, yet little is known about AdWRKYs response to drought stress in A. duranensis. In this study, RNA-seq identified five AdWRKYs, including AdWRKY18, AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, which were upregulated under drought stress. Orthologous relationships between AdWRKYs and Arabidopsis WRKY were determined to predict the regulatory networks of the five AdWRKYs based on AtWRKYs. Additionally, protein–protein interactions were predicted using differentially expressed proteins from RNA-seq. The quantitative real-time PCR (qRT-PCR) results showed that AdWRKY40 was upregulated, while AdWRKY42, AdWRKY56, and AdWRKY64 were downregulated at different time-points under drought stress. The predicted regulatory networks showed that AdWRKY40 activates COR47, RD21, and RD29A expression under drought stress. Besides, AdWRKY56 regulated CesA8 under drought stress. Aradu.YIQ80 (NAC019) interacted with AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, while Aradu.Z5H58 (NAC055) interacted with AdWRKY42 and AdWRKY64 under drought stress. This study used Arabidopsis to assess AdWRKYs function and regulatory networks, providing a basis for understanding drought tolerance in A. duranensis.https://www.frontiersin.org/articles/10.3389/fpls.2022.910408/fullArachis duranensisdrought toleranceprotein–protein interactionregulatory networkWRKY |
| spellingShingle | Yongli Zhang Pei Du Faqian Xiong Xiaojun Zhang Hui Song WRKY Genes Improve Drought Tolerance in Arachis duranensis Frontiers in Plant Science Arachis duranensis drought tolerance protein–protein interaction regulatory network WRKY |
| title | WRKY Genes Improve Drought Tolerance in Arachis duranensis |
| title_full | WRKY Genes Improve Drought Tolerance in Arachis duranensis |
| title_fullStr | WRKY Genes Improve Drought Tolerance in Arachis duranensis |
| title_full_unstemmed | WRKY Genes Improve Drought Tolerance in Arachis duranensis |
| title_short | WRKY Genes Improve Drought Tolerance in Arachis duranensis |
| title_sort | wrky genes improve drought tolerance in arachis duranensis |
| topic | Arachis duranensis drought tolerance protein–protein interaction regulatory network WRKY |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.910408/full |
| work_keys_str_mv | AT yonglizhang wrkygenesimprovedroughttoleranceinarachisduranensis AT peidu wrkygenesimprovedroughttoleranceinarachisduranensis AT faqianxiong wrkygenesimprovedroughttoleranceinarachisduranensis AT xiaojunzhang wrkygenesimprovedroughttoleranceinarachisduranensis AT huisong wrkygenesimprovedroughttoleranceinarachisduranensis |