A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.)
As a plant-specific Rho-like small G protein, the ROP (Rho-related GTPase of plants) protein regulates the growth and development of plants and various stress responses in the form of molecular switches. Drought is a major abiotic stress that limits cotton yield and fiber quality. In this study, vir...
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MDPI AG
2022-06-01
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| author | Ziyao Hu Jianfeng Lei Peihong Dai Chao Liu Abuduweili Wugalihan Xiaodong Liu Yue Li |
| author_facet | Ziyao Hu Jianfeng Lei Peihong Dai Chao Liu Abuduweili Wugalihan Xiaodong Liu Yue Li |
| author_sort | Ziyao Hu |
| collection | DOAJ |
| description | As a plant-specific Rho-like small G protein, the ROP (Rho-related GTPase of plants) protein regulates the growth and development of plants and various stress responses in the form of molecular switches. Drought is a major abiotic stress that limits cotton yield and fiber quality. In this study, virus-induced gene silencing (VIGS) technology was used to analyze the biological function of <i>GhROP3</i> in cotton drought stress tolerance. Meanwhile, we used yeast two-hybrid and bimolecular fluorescence complementation assays to examine the interaction between GhROP3 and GhGGB. <i>GhROP3</i> has a high expression level in cotton true leaves and roots, and responds to drought, high salt, cold, heat stress, and exogenous abscisic acid (ABA) and auxin (IAA) treatments. Silencing <i>GhROP3</i> improved the drought tolerance of cotton. The water loss rates (WLR) of detached leaves significantly reduced in silenced plants. Also, the relative water content (RWC) and total contents of chlorophyll (Chl) and proline (Pro) of leaves after drought stress and the activities of three antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) significantly increased, whereas the contents of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) significantly reduced. In the leaves of silenced plants, the expression of genes related to ABA synthesis and its related pathway was significantly upregulated, and the expression of decomposition-related <i>GhCYP707A</i> gene and genes related to IAA synthesis and its related pathways was significantly downregulated. It indicated that <i>GhROP3</i> was a negative regulator of cotton response to drought by participating in the negative regulation of the ABA signaling pathway and the positive regulation of the IAA signaling pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhROP3 protein interacted with the GhGGB protein in vivo and in vitro. This study provided a theoretical basis for the in-depth investigation of the drought resistance–related molecular mechanism of the <i>GhROP3</i> gene and the biological function of the <i>GhGGB</i> gene. |
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| spelling | doaj.art-e60780b4886546978c6f4f063598e6542023-11-23T18:34:56ZengMDPI AGPlants2223-77472022-06-011112158010.3390/plants11121580A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.)Ziyao Hu0Jianfeng Lei1Peihong Dai2Chao Liu3Abuduweili Wugalihan4Xiaodong Liu5Yue Li6College of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaCollege of Agronomy, Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaCollege of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaCollege of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaCollege of Agronomy, Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaCollege of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaCollege of Life Science, Xinjiang Agricultural University, Nongda East Road, Urumqi 830001, ChinaAs a plant-specific Rho-like small G protein, the ROP (Rho-related GTPase of plants) protein regulates the growth and development of plants and various stress responses in the form of molecular switches. Drought is a major abiotic stress that limits cotton yield and fiber quality. In this study, virus-induced gene silencing (VIGS) technology was used to analyze the biological function of <i>GhROP3</i> in cotton drought stress tolerance. Meanwhile, we used yeast two-hybrid and bimolecular fluorescence complementation assays to examine the interaction between GhROP3 and GhGGB. <i>GhROP3</i> has a high expression level in cotton true leaves and roots, and responds to drought, high salt, cold, heat stress, and exogenous abscisic acid (ABA) and auxin (IAA) treatments. Silencing <i>GhROP3</i> improved the drought tolerance of cotton. The water loss rates (WLR) of detached leaves significantly reduced in silenced plants. Also, the relative water content (RWC) and total contents of chlorophyll (Chl) and proline (Pro) of leaves after drought stress and the activities of three antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) significantly increased, whereas the contents of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) significantly reduced. In the leaves of silenced plants, the expression of genes related to ABA synthesis and its related pathway was significantly upregulated, and the expression of decomposition-related <i>GhCYP707A</i> gene and genes related to IAA synthesis and its related pathways was significantly downregulated. It indicated that <i>GhROP3</i> was a negative regulator of cotton response to drought by participating in the negative regulation of the ABA signaling pathway and the positive regulation of the IAA signaling pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhROP3 protein interacted with the GhGGB protein in vivo and in vitro. This study provided a theoretical basis for the in-depth investigation of the drought resistance–related molecular mechanism of the <i>GhROP3</i> gene and the biological function of the <i>GhGGB</i> gene.https://www.mdpi.com/2223-7747/11/12/1580cottondrought stress<i>GhROP3</i><i>GhGGB</i>virus-induced gene silencing (VIGS) |
| spellingShingle | Ziyao Hu Jianfeng Lei Peihong Dai Chao Liu Abuduweili Wugalihan Xiaodong Liu Yue Li A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.) Plants cotton drought stress <i>GhROP3</i> <i>GhGGB</i> virus-induced gene silencing (VIGS) |
| title | A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.) |
| title_full | A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.) |
| title_fullStr | A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.) |
| title_full_unstemmed | A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.) |
| title_short | A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (<i>Gossypium hirsutum</i> L.) |
| title_sort | small gtp binding protein ghrop3 interacts with ghggb protein and negatively regulates drought tolerance in cotton i gossypium hirsutum i l |
| topic | cotton drought stress <i>GhROP3</i> <i>GhGGB</i> virus-induced gene silencing (VIGS) |
| url | https://www.mdpi.com/2223-7747/11/12/1580 |
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