Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/5/905 |
| _version_ | 1797568575050874880 |
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| author | Saquib Ahmed M. A. Peerzade Nadezda Makarova Igor Sokolov |
| author_facet | Saquib Ahmed M. A. Peerzade Nadezda Makarova Igor Sokolov |
| author_sort | Saquib Ahmed M. A. Peerzade |
| collection | DOAJ |
| description | Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging. |
| first_indexed | 2024-03-10T19:58:52Z |
| format | Article |
| id | doaj.art-e60799f9a73e46e795d6817d9ca85ad4 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T19:58:52Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-e60799f9a73e46e795d6817d9ca85ad42023-11-19T23:46:57ZengMDPI AGNanomaterials2079-49912020-05-0110590510.3390/nano10050905Ultrabright Fluorescent Silica Nanoparticles for Multiplexed DetectionSaquib Ahmed M. A. Peerzade0Nadezda Makarova1Igor Sokolov2Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USADepartment of Mechanical Engineering, Tufts University, Medford, MA 02155, USADepartment of Biomedical Engineering, Tufts University, Medford, MA 02155, USAFluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging.https://www.mdpi.com/2079-4991/10/5/905fluorescent nanoparticlesfluorescencemultiplexing |
| spellingShingle | Saquib Ahmed M. A. Peerzade Nadezda Makarova Igor Sokolov Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection Nanomaterials fluorescent nanoparticles fluorescence multiplexing |
| title | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
| title_full | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
| title_fullStr | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
| title_full_unstemmed | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
| title_short | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
| title_sort | ultrabright fluorescent silica nanoparticles for multiplexed detection |
| topic | fluorescent nanoparticles fluorescence multiplexing |
| url | https://www.mdpi.com/2079-4991/10/5/905 |
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