Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species
The growing global threat of antimicrobial resistance is reaching a crisis point as common bacterial infections, including those caused by pathogenic <i>Neisseria</i> species, are becoming increasingly untreatable. This is compelling the scientific community to search for new antimicrobi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/11/1592 |
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| author | Elvis Achondou Akomoneh Zina Gestels Saïd Abdellati Katleen Vereecken Koen Bartholomeeusen Dorien Van den Bossche Chris Kenyon Sheeba Santhini Manoharan-Basil |
| author_facet | Elvis Achondou Akomoneh Zina Gestels Saïd Abdellati Katleen Vereecken Koen Bartholomeeusen Dorien Van den Bossche Chris Kenyon Sheeba Santhini Manoharan-Basil |
| author_sort | Elvis Achondou Akomoneh |
| collection | DOAJ |
| description | The growing global threat of antimicrobial resistance is reaching a crisis point as common bacterial infections, including those caused by pathogenic <i>Neisseria</i> species, are becoming increasingly untreatable. This is compelling the scientific community to search for new antimicrobial agents, taking advantage of computational mining and using whole genome sequences to discover natural products from the human microbiome with antibiotic effects. In this study, we investigated the crude extract from a <i>Rothia dentocariosa</i> strain with demonstrated antimicrobial activity against pathogenic <i>Neisseria</i> spp. by spot-on-lawn assay. The genomic DNA of the <i>R. dentocariosa</i> strain was sequenced, and bioinformatic evaluation was performed using antiSMASH and PRISM to search for biosynthetic gene clusters (BGCs). The crude extract with potential antimicrobial activity was run on Tricine-SDS-PAGE, and the putative peptides were characterised using liquid chromatography–tandem mass spectrometry (LC-MS). The crude extract inhibited the growth of the pathogenic <i>Neisseria</i> spp. Six BGCs were identified corresponding to non-ribosomal peptide synthases (NRPSs), polyketide synthases (PKSs), and ribosomally synthesised and post-translationally modified peptides. Three peptides were also identified corresponding to Actinorhodin polyketide putative beta-ketoacyl synthase 1. These findings serve as a useful reference to facilitate the research and development of NRPS and PKS as antimicrobial products against multidrug-resistant <i>N. gonorrhoeae.</i> |
| first_indexed | 2024-03-09T17:04:59Z |
| format | Article |
| id | doaj.art-e608955a6e25461b8c07f47b1b8ad0fc |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T17:04:59Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-e608955a6e25461b8c07f47b1b8ad0fc2023-11-24T14:25:15ZengMDPI AGAntibiotics2079-63822023-11-011211159210.3390/antibiotics12111592Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> SpeciesElvis Achondou Akomoneh0Zina Gestels1Saïd Abdellati2Katleen Vereecken3Koen Bartholomeeusen4Dorien Van den Bossche5Chris Kenyon6Sheeba Santhini Manoharan-Basil7HIV/STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine Antwerp, 2000 Antwerp, BelgiumHIV/STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine Antwerp, 2000 Antwerp, BelgiumClinical Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumVirology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumVirology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumClinical Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumHIV/STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine Antwerp, 2000 Antwerp, BelgiumHIV/STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine Antwerp, 2000 Antwerp, BelgiumThe growing global threat of antimicrobial resistance is reaching a crisis point as common bacterial infections, including those caused by pathogenic <i>Neisseria</i> species, are becoming increasingly untreatable. This is compelling the scientific community to search for new antimicrobial agents, taking advantage of computational mining and using whole genome sequences to discover natural products from the human microbiome with antibiotic effects. In this study, we investigated the crude extract from a <i>Rothia dentocariosa</i> strain with demonstrated antimicrobial activity against pathogenic <i>Neisseria</i> spp. by spot-on-lawn assay. The genomic DNA of the <i>R. dentocariosa</i> strain was sequenced, and bioinformatic evaluation was performed using antiSMASH and PRISM to search for biosynthetic gene clusters (BGCs). The crude extract with potential antimicrobial activity was run on Tricine-SDS-PAGE, and the putative peptides were characterised using liquid chromatography–tandem mass spectrometry (LC-MS). The crude extract inhibited the growth of the pathogenic <i>Neisseria</i> spp. Six BGCs were identified corresponding to non-ribosomal peptide synthases (NRPSs), polyketide synthases (PKSs), and ribosomally synthesised and post-translationally modified peptides. Three peptides were also identified corresponding to Actinorhodin polyketide putative beta-ketoacyl synthase 1. These findings serve as a useful reference to facilitate the research and development of NRPS and PKS as antimicrobial products against multidrug-resistant <i>N. gonorrhoeae.</i>https://www.mdpi.com/2079-6382/12/11/1592NRPSPKSnatural antimicrobials<i>Rothia dentocariosa</i><i>Neisseria gonorrhoeae</i> |
| spellingShingle | Elvis Achondou Akomoneh Zina Gestels Saïd Abdellati Katleen Vereecken Koen Bartholomeeusen Dorien Van den Bossche Chris Kenyon Sheeba Santhini Manoharan-Basil Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species Antibiotics NRPS PKS natural antimicrobials <i>Rothia dentocariosa</i> <i>Neisseria gonorrhoeae</i> |
| title | Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species |
| title_full | Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species |
| title_fullStr | Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species |
| title_full_unstemmed | Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species |
| title_short | Genome Mining Uncovers NRPS and PKS Clusters in <i>Rothia dentocariosa</i> with Inhibitory Activity against <i>Neisseria</i> Species |
| title_sort | genome mining uncovers nrps and pks clusters in i rothia dentocariosa i with inhibitory activity against i neisseria i species |
| topic | NRPS PKS natural antimicrobials <i>Rothia dentocariosa</i> <i>Neisseria gonorrhoeae</i> |
| url | https://www.mdpi.com/2079-6382/12/11/1592 |
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