Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice
This study aimed to investigate the preventive effects of lactoferrin (Lf) on chronic alcoholic liver injury (ALI) in female mice. Female C57BL/6J mice were randomly divided into four groups: control group (CON), ethanol administration group (EtOH), low-dose Lf treatment group (LLf), and high-dose L...
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MDPI AG
2022-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/8/1508 |
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| author | De-Ming Li Yun-Xuan Wu Zhi-Qiang Hu Tian-Ci Wang Li-Li Zhang Yan Zhou Xing Tong Jia-Ying Xu Li-Qiang Qin |
| author_facet | De-Ming Li Yun-Xuan Wu Zhi-Qiang Hu Tian-Ci Wang Li-Li Zhang Yan Zhou Xing Tong Jia-Ying Xu Li-Qiang Qin |
| author_sort | De-Ming Li |
| collection | DOAJ |
| description | This study aimed to investigate the preventive effects of lactoferrin (Lf) on chronic alcoholic liver injury (ALI) in female mice. Female C57BL/6J mice were randomly divided into four groups: control group (CON), ethanol administration group (EtOH), low-dose Lf treatment group (LLf), and high-dose Lf group (HLf). In the last three groups, chronic ALI was induced by administering 20% ethanol ad libitum for 12 weeks. Mice in the CON and EtOH groups were fed with AIN-93G diet. Meanwhile, 0.4% and 4% casein in the AIN-93G diet were replaced by Lf as the diets of LLf and HLf groups, respectively. HLf significantly reduced hepatic triglyceride content and improved pathological morphology. HLf could inhibit cytochrome P450 2E1 overexpression and promote alcohol dehydrogenase-1 expression. HLf activated protein kinase B and AMP-activated protein kinase (AMPK), as well as upregulating nuclear-factor-erythroid-2-related factor-2 expression to elevate hepatic antioxidative enzyme activities. AMPK activation also benefited hepatic lipid metabolism. Meanwhile, HLf had no obvious beneficial effects on gut microbiota. In summary, Lf could alleviate chronic ALI in female mice, which was associated with redox balance and lipid metabolism regulation. |
| first_indexed | 2024-03-09T11:59:06Z |
| format | Article |
| id | doaj.art-e6092565e64141cc9890f976ebe3edb8 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:59:06Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-e6092565e64141cc9890f976ebe3edb82023-11-30T23:06:02ZengMDPI AGAntioxidants2076-39212022-07-01118150810.3390/antiox11081508Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J MiceDe-Ming Li0Yun-Xuan Wu1Zhi-Qiang Hu2Tian-Ci Wang3Li-Li Zhang4Yan Zhou5Xing Tong6Jia-Ying Xu7Li-Qiang Qin8School of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaLaboratory Center, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaState Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaThis study aimed to investigate the preventive effects of lactoferrin (Lf) on chronic alcoholic liver injury (ALI) in female mice. Female C57BL/6J mice were randomly divided into four groups: control group (CON), ethanol administration group (EtOH), low-dose Lf treatment group (LLf), and high-dose Lf group (HLf). In the last three groups, chronic ALI was induced by administering 20% ethanol ad libitum for 12 weeks. Mice in the CON and EtOH groups were fed with AIN-93G diet. Meanwhile, 0.4% and 4% casein in the AIN-93G diet were replaced by Lf as the diets of LLf and HLf groups, respectively. HLf significantly reduced hepatic triglyceride content and improved pathological morphology. HLf could inhibit cytochrome P450 2E1 overexpression and promote alcohol dehydrogenase-1 expression. HLf activated protein kinase B and AMP-activated protein kinase (AMPK), as well as upregulating nuclear-factor-erythroid-2-related factor-2 expression to elevate hepatic antioxidative enzyme activities. AMPK activation also benefited hepatic lipid metabolism. Meanwhile, HLf had no obvious beneficial effects on gut microbiota. In summary, Lf could alleviate chronic ALI in female mice, which was associated with redox balance and lipid metabolism regulation.https://www.mdpi.com/2076-3921/11/8/1508lactoferrinchronic alcoholic liver injuryredox balancelipid metabolismfemale mice |
| spellingShingle | De-Ming Li Yun-Xuan Wu Zhi-Qiang Hu Tian-Ci Wang Li-Li Zhang Yan Zhou Xing Tong Jia-Ying Xu Li-Qiang Qin Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice Antioxidants lactoferrin chronic alcoholic liver injury redox balance lipid metabolism female mice |
| title | Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice |
| title_full | Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice |
| title_fullStr | Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice |
| title_full_unstemmed | Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice |
| title_short | Lactoferrin Prevents Chronic Alcoholic Injury by Regulating Redox Balance and Lipid Metabolism in Female C57BL/6J Mice |
| title_sort | lactoferrin prevents chronic alcoholic injury by regulating redox balance and lipid metabolism in female c57bl 6j mice |
| topic | lactoferrin chronic alcoholic liver injury redox balance lipid metabolism female mice |
| url | https://www.mdpi.com/2076-3921/11/8/1508 |
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