Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear
Cold stress is a prominent abiotic factor that adversely affects the growth and yield of pears, consequently restricting the cultivation range and resulting in substantial economic losses for the pear industry. Basic region–leucine zipper (bZIP) transcription factors are widely involved in multiple...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/1/24 |
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| author | Yuxin Zhang Lin Wu Lun Liu Bing Jia Zhenfeng Ye Xiaomei Tang Wei Heng Li Liu |
| author_facet | Yuxin Zhang Lin Wu Lun Liu Bing Jia Zhenfeng Ye Xiaomei Tang Wei Heng Li Liu |
| author_sort | Yuxin Zhang |
| collection | DOAJ |
| description | Cold stress is a prominent abiotic factor that adversely affects the growth and yield of pears, consequently restricting the cultivation range and resulting in substantial economic losses for the pear industry. Basic region–leucine zipper (bZIP) transcription factors are widely involved in multiple physiological and biochemical activities of plants, particularly in response to cold stress. In this study, the responsiveness of <i>PbbZIP11</i> in pear to cold stress was investigated, and its role was explored by using pear callus and <i>Arabidopsis thaliana</i>. The findings revealed that overexpression of <i>PbbZIP11</i> enhanced the tolerance of pear callus and <i>Arabidopsis thaliana</i> to cold stress. The antioxidant enzyme activities of transgenic plants were enhanced and the expression of C-repeat binding transcription factor (CBF) genes was increased as compared to wild-type plants. To better understand the biological function of <i>PbbZIP11</i>, mRNAs were isolated from overexpressed and wild-type <i>Arabidopsis thaliana</i> after cold stress for whole-genome sequencing. The results showed that the expression of some CBF downstream target genes changed after exposure to cold stress. The results suggested that the <i>PbbZIP11</i> gene could participate in cold-stress signaling through the CBF-dependent pathway, which provides a theoretical basis for the <i>PbbZIP11</i>-mediated response to cold stress and for the genetic breeding of pear varieties with low-temperature tolerance. |
| first_indexed | 2024-03-08T14:59:48Z |
| format | Article |
| id | doaj.art-e609d841a2694ce2a440fe11ff1e12bb |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T14:59:48Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-e609d841a2694ce2a440fe11ff1e12bb2024-01-10T15:05:57ZengMDPI AGPlants2223-77472023-12-011312410.3390/plants13010024Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and PearYuxin Zhang0Lin Wu1Lun Liu2Bing Jia3Zhenfeng Ye4Xiaomei Tang5Wei Heng6Li Liu7College of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCollege of Horticulture, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, ChinaCold stress is a prominent abiotic factor that adversely affects the growth and yield of pears, consequently restricting the cultivation range and resulting in substantial economic losses for the pear industry. Basic region–leucine zipper (bZIP) transcription factors are widely involved in multiple physiological and biochemical activities of plants, particularly in response to cold stress. In this study, the responsiveness of <i>PbbZIP11</i> in pear to cold stress was investigated, and its role was explored by using pear callus and <i>Arabidopsis thaliana</i>. The findings revealed that overexpression of <i>PbbZIP11</i> enhanced the tolerance of pear callus and <i>Arabidopsis thaliana</i> to cold stress. The antioxidant enzyme activities of transgenic plants were enhanced and the expression of C-repeat binding transcription factor (CBF) genes was increased as compared to wild-type plants. To better understand the biological function of <i>PbbZIP11</i>, mRNAs were isolated from overexpressed and wild-type <i>Arabidopsis thaliana</i> after cold stress for whole-genome sequencing. The results showed that the expression of some CBF downstream target genes changed after exposure to cold stress. The results suggested that the <i>PbbZIP11</i> gene could participate in cold-stress signaling through the CBF-dependent pathway, which provides a theoretical basis for the <i>PbbZIP11</i>-mediated response to cold stress and for the genetic breeding of pear varieties with low-temperature tolerance.https://www.mdpi.com/2223-7747/13/1/24pearcold stress<i>PbbZIP11</i>CBF-dependent signaling |
| spellingShingle | Yuxin Zhang Lin Wu Lun Liu Bing Jia Zhenfeng Ye Xiaomei Tang Wei Heng Li Liu Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear Plants pear cold stress <i>PbbZIP11</i> CBF-dependent signaling |
| title | Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear |
| title_full | Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear |
| title_fullStr | Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear |
| title_full_unstemmed | Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear |
| title_short | Functional Analysis of <i>PbbZIP11</i> Transcription Factor in Response to Cold Stress in Arabidopsis and Pear |
| title_sort | functional analysis of i pbbzip11 i transcription factor in response to cold stress in arabidopsis and pear |
| topic | pear cold stress <i>PbbZIP11</i> CBF-dependent signaling |
| url | https://www.mdpi.com/2223-7747/13/1/24 |
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