Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep
Silage is widely used as ruminant feed all over the world. Lactic acid bacteria inoculants are commonly applied in silage production to improve preservation efficiency. To investigate the effects of lactic acid bacteria-inoculated silage on the bacterial communities and metabolites of the digestive...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/7/320 |
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| author | Hongyan Han Chao Wang Zhipeng Huang Ying Zhang Lin Sun Yanlin Xue Xusheng Guo |
| author_facet | Hongyan Han Chao Wang Zhipeng Huang Ying Zhang Lin Sun Yanlin Xue Xusheng Guo |
| author_sort | Hongyan Han |
| collection | DOAJ |
| description | Silage is widely used as ruminant feed all over the world. Lactic acid bacteria inoculants are commonly applied in silage production to improve preservation efficiency. To investigate the effects of lactic acid bacteria-inoculated silage on the bacterial communities and metabolites of the digestive tract, twenty-four local hybrid rams (a hybrid of Small Tail Han sheep and Mongolian sheep with an average initial BW 27.8 ± 3.05 kg) were randomly divided into four groups fed with corn stalk (S), corn stalk silage, corn stalk silage treated with <i>Lentilactobacillus</i><i>plantarum</i> (P), or corn stalk silage treated with <i>L. buchneri</i> (B). The results showed that compared with the control and B groups, the inoculant with P significantly increased silage dry matter (DM) content, while the pH value was significantly higher than that in group B, and the aerobic stability was significantly lower than that in group B. Firmicutes and Bacteroidetes were the two dominant phyla of digestive tract microbiota in sheep. Compared with corn stalk, sheep fed with corn stalk silage showed a higher relative abundance of <i>Prevotella</i>. P-treated silage decreased the relative abundance of Firmicutes at the phylum level in rumen fluid. Silage treated with P or B increased the relative abundance of <i>Prevotella</i>, <i>Ruminococcus</i>, and <i>Fibrobacter</i> at the genus level in the rumen. A total of 498 differential metabolites in the rumen were detected when comparing the corn stalk and corn stalk silage groups. A total of 257 and 141 differential metabolites were detected when comparing the untreated silage and silages treated with P and B, respectively. These metabolites in the sheep rumen were correlated with bacterial communities, especially <i>Butyrivibrio</i>, <i>Fibrobacter,</i> and <i>Prevotella</i>. In conclusion, the addition of P and B during ensiling could change the fermentation and metabolites in the rumen by influencing the bacterial community. The change induced by these inoculants might be beneficial for animals’ performance and the health of ruminants. |
| first_indexed | 2024-03-09T03:26:52Z |
| format | Article |
| id | doaj.art-e60b308ce0b6400ba11f6d5ddb774c73 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T03:26:52Z |
| publishDate | 2022-07-01 |
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| series | Fermentation |
| spelling | doaj.art-e60b308ce0b6400ba11f6d5ddb774c732023-12-03T15:01:47ZengMDPI AGFermentation2311-56372022-07-018732010.3390/fermentation8070320Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of SheepHongyan Han0Chao Wang1Zhipeng Huang2Ying Zhang3Lin Sun4Yanlin Xue5Xusheng Guo6State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaInner Mongolia Engineering Research Center of Development and Utilization of Microbial Resources in Silage, Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Inner Mongolia Key Laboratory of Microbial Ecology of Silage, Hohhot 010031, ChinaState Key Laboratory of Grassland and Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730000, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaInner Mongolia Engineering Research Center of Development and Utilization of Microbial Resources in Silage, Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Inner Mongolia Key Laboratory of Microbial Ecology of Silage, Hohhot 010031, ChinaInner Mongolia Engineering Research Center of Development and Utilization of Microbial Resources in Silage, Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Inner Mongolia Key Laboratory of Microbial Ecology of Silage, Hohhot 010031, ChinaState Key Laboratory of Grassland and Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730000, ChinaSilage is widely used as ruminant feed all over the world. Lactic acid bacteria inoculants are commonly applied in silage production to improve preservation efficiency. To investigate the effects of lactic acid bacteria-inoculated silage on the bacterial communities and metabolites of the digestive tract, twenty-four local hybrid rams (a hybrid of Small Tail Han sheep and Mongolian sheep with an average initial BW 27.8 ± 3.05 kg) were randomly divided into four groups fed with corn stalk (S), corn stalk silage, corn stalk silage treated with <i>Lentilactobacillus</i><i>plantarum</i> (P), or corn stalk silage treated with <i>L. buchneri</i> (B). The results showed that compared with the control and B groups, the inoculant with P significantly increased silage dry matter (DM) content, while the pH value was significantly higher than that in group B, and the aerobic stability was significantly lower than that in group B. Firmicutes and Bacteroidetes were the two dominant phyla of digestive tract microbiota in sheep. Compared with corn stalk, sheep fed with corn stalk silage showed a higher relative abundance of <i>Prevotella</i>. P-treated silage decreased the relative abundance of Firmicutes at the phylum level in rumen fluid. Silage treated with P or B increased the relative abundance of <i>Prevotella</i>, <i>Ruminococcus</i>, and <i>Fibrobacter</i> at the genus level in the rumen. A total of 498 differential metabolites in the rumen were detected when comparing the corn stalk and corn stalk silage groups. A total of 257 and 141 differential metabolites were detected when comparing the untreated silage and silages treated with P and B, respectively. These metabolites in the sheep rumen were correlated with bacterial communities, especially <i>Butyrivibrio</i>, <i>Fibrobacter,</i> and <i>Prevotella</i>. In conclusion, the addition of P and B during ensiling could change the fermentation and metabolites in the rumen by influencing the bacterial community. The change induced by these inoculants might be beneficial for animals’ performance and the health of ruminants.https://www.mdpi.com/2311-5637/8/7/320lactic acid bacteriasilagerumenbacterial communitymetabolites |
| spellingShingle | Hongyan Han Chao Wang Zhipeng Huang Ying Zhang Lin Sun Yanlin Xue Xusheng Guo Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep Fermentation lactic acid bacteria silage rumen bacterial community metabolites |
| title | Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep |
| title_full | Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep |
| title_fullStr | Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep |
| title_full_unstemmed | Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep |
| title_short | Effects of Lactic Acid Bacteria-Inoculated Corn Silage on Bacterial Communities and Metabolites of Digestive Tract of Sheep |
| title_sort | effects of lactic acid bacteria inoculated corn silage on bacterial communities and metabolites of digestive tract of sheep |
| topic | lactic acid bacteria silage rumen bacterial community metabolites |
| url | https://www.mdpi.com/2311-5637/8/7/320 |
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