Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae)
Sida linifolia L. is a valuable plant of West Tropical Africa with several folklore claims and growing evidence of its bioactivity, including its pain-relieving potential; however, scientific validation of these claims is still limited. This study investigated the phytochemical composition and analg...
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| Format: | Article |
| Language: | English |
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Bektas Tepe
2023-12-01
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| Series: | International Journal of Plant Based Pharmaceuticals |
| Subjects: | |
| Online Access: | https://ijpbp.com/index.php/ijpbp/article/view/140 |
| _version_ | 1797394441647947776 |
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| author | Emmanuel Chimeh Ezeako Abel Yashim Solomon Hope Chimbuezie Ngozi Abayomi Oyeyemi Ajagbe Linus Ogbonnaya Agwu Lomi Oruchukwu Michael Evaristus Chinonso Odoh Victor Inioluwa Olaoye Philip Nwachukwu Ashiakpa Martin Benedict Nworie Vincent E. Ozougwu Parker Elijah Joshua |
| author_facet | Emmanuel Chimeh Ezeako Abel Yashim Solomon Hope Chimbuezie Ngozi Abayomi Oyeyemi Ajagbe Linus Ogbonnaya Agwu Lomi Oruchukwu Michael Evaristus Chinonso Odoh Victor Inioluwa Olaoye Philip Nwachukwu Ashiakpa Martin Benedict Nworie Vincent E. Ozougwu Parker Elijah Joshua |
| author_sort | Emmanuel Chimeh Ezeako |
| collection | DOAJ |
| description | Sida linifolia L. is a valuable plant of West Tropical Africa with several folklore claims and growing evidence of its bioactivity, including its pain-relieving potential; however, scientific validation of these claims is still limited. This study investigated the phytochemical composition and analgesic potential of the ethyl acetate fraction of the hydro-alcoholic extract of S. linifolia leaves (SEAL). Gas chromatography-flame ionization detector (GC-FID) and high-performance liquid chromatography (HPLC) techniques were used to determine the phytochemistry of SEAL. Formalin and acetic acid models were employed to determine the analgesic properties of SEAL. The result of GC-FID analysis revealed varying concentrations of lunamarine, naringenin, ephedrine, catechins, cardiac glycosides, flavanones, kaempferol, flavones, phytate, rutin, steroids, tannins, oxalate, flavan-3-ols, sapogenins, cyanogenic glycosides, anthocyanin, and proanthocyanidins in SEAL. In addition, varying concentrations of polyphenolics, such as phenylacetic acid, caffeic acid, ellagic acid, and naringenin were detected in SEAL via HPLC analysis. The LD50 study showed that SEAL was safe up to 5000 mg/kg body weight per oral (p.o.) in Swiss mice. Pretreatment with oral doses (200, 400, and 600 mg/kg bw) of SEAL significantly (p < 0.05) inhibited all phases of formalin-induced hind paw licking and acetic acid-induced writhing syndrome in mice compared to positive control and was on par with aspirin (100 mg/kg bw p.o.). The observed bioactivity of SEAL could be anchored to its phytoconstituents. Therefore, the plant leaf fraction represents a good source of bioactive compounds with immense potential for exploration in therapeutic research. |
| first_indexed | 2024-03-09T00:19:47Z |
| format | Article |
| id | doaj.art-e60d4142cfaf4b82b5a6bdcd10020a04 |
| institution | Directory Open Access Journal |
| issn | 2791-7509 |
| language | English |
| last_indexed | 2024-03-09T00:19:47Z |
| publishDate | 2023-12-01 |
| publisher | Bektas Tepe |
| record_format | Article |
| series | International Journal of Plant Based Pharmaceuticals |
| spelling | doaj.art-e60d4142cfaf4b82b5a6bdcd10020a042023-12-12T05:36:22ZengBektas TepeInternational Journal of Plant Based Pharmaceuticals2791-75092023-12-013222823910.29228/ijpbp.36140Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae)Emmanuel Chimeh Ezeako0https://orcid.org/0000-0002-9675-8320Abel Yashim Solomon1https://orcid.org/0000-0002-7318-5961Hope Chimbuezie Ngozi2https://orcid.org/0000-0001-7385-1576Abayomi Oyeyemi Ajagbe3https://orcid.org/0000-0002-2110-2626Linus Ogbonnaya Agwu4https://orcid.org/0000-0002-9208-2917Lomi Oruchukwu Michael5https://orcid.org/0009-0006-6471-3335Evaristus Chinonso Odoh6https://orcid.org/0000-0002-5684-2931Victor Inioluwa Olaoye7https://orcid.org/0000-0001-5892-6111Philip Nwachukwu Ashiakpa8https://orcid.org/0000-0002-5149-8000Martin Benedict Nworie9https://orcid.org/0000-0003-4262-9029Vincent E. Ozougwu10https://orcid.org/0000-0003-0663-8796Parker Elijah Joshua11https://orcid.org/0000-0002-1837-0312University of Nigeria, Department of Biochemistry, Nsukka, Enugu, NigeriaNile University, College of Health Sciences, Department of Anatomy, Abuja, NigeriaUniversity of Nigeria, Department of Microbiology, Nsukka, Enugu, NigeriaNile University, College of Health Sciences, Department of Anatomy, Abuja, NigeriaFederal University of Technology, Owerri, Imo, NigeriaUniversity of Nigeria, Department of Biochemistry, Nsukka, Enugu, NigeriaModibbo Adama University Teaching Hospital, Department of Pharmacy, Yola, Adamawa, NigeriaUniversity of Ibadan, Department of Chemistry, Ibadan, NigeriaUniversity of Nigeria, Department of Biochemistry, Nsukka, Enugu, NigeriaUniversity of Nigeria, Department of Biochemistry, Nsukka, Enugu, NigeriaUniversity of Nigeria, Department of Biochemistry, Nsukka, Enugu, NigeriaUniversity of Nigeria, Department of Biochemistry, Nsukka, Enugu, NigeriaSida linifolia L. is a valuable plant of West Tropical Africa with several folklore claims and growing evidence of its bioactivity, including its pain-relieving potential; however, scientific validation of these claims is still limited. This study investigated the phytochemical composition and analgesic potential of the ethyl acetate fraction of the hydro-alcoholic extract of S. linifolia leaves (SEAL). Gas chromatography-flame ionization detector (GC-FID) and high-performance liquid chromatography (HPLC) techniques were used to determine the phytochemistry of SEAL. Formalin and acetic acid models were employed to determine the analgesic properties of SEAL. The result of GC-FID analysis revealed varying concentrations of lunamarine, naringenin, ephedrine, catechins, cardiac glycosides, flavanones, kaempferol, flavones, phytate, rutin, steroids, tannins, oxalate, flavan-3-ols, sapogenins, cyanogenic glycosides, anthocyanin, and proanthocyanidins in SEAL. In addition, varying concentrations of polyphenolics, such as phenylacetic acid, caffeic acid, ellagic acid, and naringenin were detected in SEAL via HPLC analysis. The LD50 study showed that SEAL was safe up to 5000 mg/kg body weight per oral (p.o.) in Swiss mice. Pretreatment with oral doses (200, 400, and 600 mg/kg bw) of SEAL significantly (p < 0.05) inhibited all phases of formalin-induced hind paw licking and acetic acid-induced writhing syndrome in mice compared to positive control and was on par with aspirin (100 mg/kg bw p.o.). The observed bioactivity of SEAL could be anchored to its phytoconstituents. Therefore, the plant leaf fraction represents a good source of bioactive compounds with immense potential for exploration in therapeutic research.https://ijpbp.com/index.php/ijpbp/article/view/140sida linifoliaphytocompoundspolyphenolicsanalgesicmedicinal plant |
| spellingShingle | Emmanuel Chimeh Ezeako Abel Yashim Solomon Hope Chimbuezie Ngozi Abayomi Oyeyemi Ajagbe Linus Ogbonnaya Agwu Lomi Oruchukwu Michael Evaristus Chinonso Odoh Victor Inioluwa Olaoye Philip Nwachukwu Ashiakpa Martin Benedict Nworie Vincent E. Ozougwu Parker Elijah Joshua Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae) International Journal of Plant Based Pharmaceuticals sida linifolia phytocompounds polyphenolics analgesic medicinal plant |
| title | Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae) |
| title_full | Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae) |
| title_fullStr | Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae) |
| title_full_unstemmed | Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae) |
| title_short | Assessment of the analgesic potential of ethylacetate leaf fraction of Sida linifolia L. (Malvaceae) |
| title_sort | assessment of the analgesic potential of ethylacetate leaf fraction of sida linifolia l malvaceae |
| topic | sida linifolia phytocompounds polyphenolics analgesic medicinal plant |
| url | https://ijpbp.com/index.php/ijpbp/article/view/140 |
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