Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test

Aim: This study was designed to evaluate the potential of the use of multiplex polymerase chain reaction (PCR) as an alternative to conventional antibiotic sensitivity test. Materials and Methods: Isolates of Staphylococcus aureus (total = 36) from clinical cases presented to Teaching Veterinary...

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Main Authors: K. Rathore, B. Joseph, D. K. Sharma, A. Gaurav, S. K. Sharma, M. Milind, P. Patel, C. Prakash, L. Singh
Format: Article
Language:English
Published: Veterinary World 2018-04-01
Series:Veterinary World
Subjects:
Online Access:http://www.veterinaryworld.org/Vol.11/April-2018/11.pdf
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author K. Rathore
B. Joseph
D. K. Sharma
A. Gaurav
S. K. Sharma
M. Milind
P. Patel
C. Prakash
L. Singh
author_facet K. Rathore
B. Joseph
D. K. Sharma
A. Gaurav
S. K. Sharma
M. Milind
P. Patel
C. Prakash
L. Singh
author_sort K. Rathore
collection DOAJ
description Aim: This study was designed to evaluate the potential of the use of multiplex polymerase chain reaction (PCR) as an alternative to conventional antibiotic sensitivity test. Materials and Methods: Isolates of Staphylococcus aureus (total = 36) from clinical cases presented to Teaching Veterinary Clinical Complex of College of Veterinary and Animal Sciences (CVAS), Navania, Udaipur, were characterized by morphological, cultural, and biochemical methods. Then, the isolates were further subjected to molecular characterization by PCR targeting S. aureus-specific sequence (107 bp). Phenotypic antibiotic sensitivity pattern was analyzed by Kirby Bauer disc diffusion method against 11 commonly used antibiotics in veterinary medicine in and around Udaipur region. The genotypic antibiotic sensitivity pattern was studied against methicillin, aminoglycosides, and tetracycline targeting the gene mecA, aacA-aphD, and tetK by multiplex PCR. Results: There was 100% correlation between the phenotype and genotype of aminoglycoside resistance, more than 90% correlation for methicillin resistance, and 58.3% in the case tetracycline resistance. Conclusion: As there is a good correlation between phenotype and genotype of antibiotic resistance, multiplex PCR can be used as an alternative to the conventional antibiotic susceptibility testing, as it can give a rapid and true prediction of antibiotic sensitivity pattern.
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spelling doaj.art-e612a3818ee04c1787a42f1fe7616a4a2022-12-21T21:35:06ZengVeterinary WorldVeterinary World0972-89882231-09162018-04-0111447447910.14202/vetworld.2018.474-479Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity testK. Rathore0B. Joseph1D. K. Sharma2A. Gaurav3S. K. Sharma4M. Milind5P. Patel6C. Prakash7L. Singh8Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Department of Veterinary Public Health, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Department of Veterinary Medicine, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Animal Health Division, CSWRI, Avikanagar, Malpura, Tonk, 304501 Rajasthan, India.Department of Livestock Products Technology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.Aim: This study was designed to evaluate the potential of the use of multiplex polymerase chain reaction (PCR) as an alternative to conventional antibiotic sensitivity test. Materials and Methods: Isolates of Staphylococcus aureus (total = 36) from clinical cases presented to Teaching Veterinary Clinical Complex of College of Veterinary and Animal Sciences (CVAS), Navania, Udaipur, were characterized by morphological, cultural, and biochemical methods. Then, the isolates were further subjected to molecular characterization by PCR targeting S. aureus-specific sequence (107 bp). Phenotypic antibiotic sensitivity pattern was analyzed by Kirby Bauer disc diffusion method against 11 commonly used antibiotics in veterinary medicine in and around Udaipur region. The genotypic antibiotic sensitivity pattern was studied against methicillin, aminoglycosides, and tetracycline targeting the gene mecA, aacA-aphD, and tetK by multiplex PCR. Results: There was 100% correlation between the phenotype and genotype of aminoglycoside resistance, more than 90% correlation for methicillin resistance, and 58.3% in the case tetracycline resistance. Conclusion: As there is a good correlation between phenotype and genotype of antibiotic resistance, multiplex PCR can be used as an alternative to the conventional antibiotic susceptibility testing, as it can give a rapid and true prediction of antibiotic sensitivity pattern.http://www.veterinaryworld.org/Vol.11/April-2018/11.pdfantimicrobial resistancegenotypephenotypeStaphylococcus aureus
spellingShingle K. Rathore
B. Joseph
D. K. Sharma
A. Gaurav
S. K. Sharma
M. Milind
P. Patel
C. Prakash
L. Singh
Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
Veterinary World
antimicrobial resistance
genotype
phenotype
Staphylococcus aureus
title Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
title_full Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
title_fullStr Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
title_full_unstemmed Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
title_short Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
title_sort evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test
topic antimicrobial resistance
genotype
phenotype
Staphylococcus aureus
url http://www.veterinaryworld.org/Vol.11/April-2018/11.pdf
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