Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels
Estrogen has multiple effects on lipid and lipoprotein metabolism. We investigated the association between the four common single nucleotide polymorphisms in the estrogen receptor 1 (ESR1) gene locus, -1989T>G, +261G>C, IVS1-397T>C and IVS1-351A>G, and lipid and lipoprotein levels in sou...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2008-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000007 |
| _version_ | 1819055052880347136 |
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| author | S. Almeida M.H. Hutz |
| author_facet | S. Almeida M.H. Hutz |
| author_sort | S. Almeida |
| collection | DOAJ |
| description | Estrogen has multiple effects on lipid and lipoprotein metabolism. We investigated the association between the four common single nucleotide polymorphisms in the estrogen receptor 1 (ESR1) gene locus, -1989T>G, +261G>C, IVS1-397T>C and IVS1-351A>G, and lipid and lipoprotein levels in southern Brazilians. The sample consisted in 150 men and 187 premenopausal women. The women were considered premenopausal if they had regular menstrual bleeding within the previous 3 months and were 18-50 years of age. Exclusion criteria were pregnancy, secondary hyperlipidemia due to renal, hepatic or thyroid disease, and diabetes. Smoking status was self-reported; subjects were classified as never smoked and current smokers. DNA was amplified by PCR and was subsequently digested with the appropriate restriction enzymes. Statistical analysis was carried out for men and women separately. In the study population, major allele frequencies were _1989*T (0.83), +261*G (0.96), IVS1-397*T (0.58), and IVS1-351*A (0.65). Multiple linear regression analyses indicated that an interaction between +261G>C polymorphism and smoking was a significant factor affecting high-density lipoprotein cholesterol (HDL-C) levels (P = 0.028) in women. Nonsmoking women with genotype G/C of +261G>C polymorphism had mean HDL-C levels higher than those with G/G genotype (1.40 ± 0.33 vs 1.22 ± 0.26 mmol/L; P = 0.033). No significant associations with lipid and lipoprotein levels in women and men were detected for other polymorphisms. In conclusion, the +261G>C polymorphism might influence lipoprotein and lipid levels in premenopausal women, but these effects seem to be modulated by smoking, whereas in men ESR1 polymorphisms were not associated with high lipoprotein levels. |
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| format | Article |
| id | doaj.art-e6133e87f8eb4969ae7a3282bd585440 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-21T13:01:24Z |
| publishDate | 2008-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-e6133e87f8eb4969ae7a3282bd5854402022-12-21T19:03:11ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2008-10-014110872876Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levelsS. AlmeidaM.H. HutzEstrogen has multiple effects on lipid and lipoprotein metabolism. We investigated the association between the four common single nucleotide polymorphisms in the estrogen receptor 1 (ESR1) gene locus, -1989T>G, +261G>C, IVS1-397T>C and IVS1-351A>G, and lipid and lipoprotein levels in southern Brazilians. The sample consisted in 150 men and 187 premenopausal women. The women were considered premenopausal if they had regular menstrual bleeding within the previous 3 months and were 18-50 years of age. Exclusion criteria were pregnancy, secondary hyperlipidemia due to renal, hepatic or thyroid disease, and diabetes. Smoking status was self-reported; subjects were classified as never smoked and current smokers. DNA was amplified by PCR and was subsequently digested with the appropriate restriction enzymes. Statistical analysis was carried out for men and women separately. In the study population, major allele frequencies were _1989*T (0.83), +261*G (0.96), IVS1-397*T (0.58), and IVS1-351*A (0.65). Multiple linear regression analyses indicated that an interaction between +261G>C polymorphism and smoking was a significant factor affecting high-density lipoprotein cholesterol (HDL-C) levels (P = 0.028) in women. Nonsmoking women with genotype G/C of +261G>C polymorphism had mean HDL-C levels higher than those with G/G genotype (1.40 ± 0.33 vs 1.22 ± 0.26 mmol/L; P = 0.033). No significant associations with lipid and lipoprotein levels in women and men were detected for other polymorphisms. In conclusion, the +261G>C polymorphism might influence lipoprotein and lipid levels in premenopausal women, but these effects seem to be modulated by smoking, whereas in men ESR1 polymorphisms were not associated with high lipoprotein levels.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000007EstrogenEstrogen receptor 1 variantsLipid levelsSmoking |
| spellingShingle | S. Almeida M.H. Hutz Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels Brazilian Journal of Medical and Biological Research Estrogen Estrogen receptor 1 variants Lipid levels Smoking |
| title | Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels |
| title_full | Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels |
| title_fullStr | Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels |
| title_full_unstemmed | Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels |
| title_short | Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels |
| title_sort | estrogen receptor 1 gene polymorphisms in premenopausal women interaction between genotype and smoking on lipid levels |
| topic | Estrogen Estrogen receptor 1 variants Lipid levels Smoking |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000007 |
| work_keys_str_mv | AT salmeida estrogenreceptor1genepolymorphismsinpremenopausalwomeninteractionbetweengenotypeandsmokingonlipidlevels AT mhhutz estrogenreceptor1genepolymorphismsinpremenopausalwomeninteractionbetweengenotypeandsmokingonlipidlevels |