Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i>
The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of <i>Saussurea controversa</i> were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isol...
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/20/3680 |
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| author | Igor Khlusov Elena Avdeeva Valeria Shupletsova Olga Khaziakhmatova Larisa Litvinova Ekaterina Porokhova Yaroslav Reshetov Irina Zvereva Lyudmila Mushtovatova Mariia Karpova Artem Guryev Irina Sukhodolo Mikhail Belousov |
| author_facet | Igor Khlusov Elena Avdeeva Valeria Shupletsova Olga Khaziakhmatova Larisa Litvinova Ekaterina Porokhova Yaroslav Reshetov Irina Zvereva Lyudmila Mushtovatova Mariia Karpova Artem Guryev Irina Sukhodolo Mikhail Belousov |
| author_sort | Igor Khlusov |
| collection | DOAJ |
| description | The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of <i>Saussurea controversa</i> were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of <i>S. controversa</i> and have been characterized previously. PS-SC was first isolated from the leaves extract of <i>S. controversa</i> and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of <i>Staphylococcus aureus</i> strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10−50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of <i>S. controversa</i> and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications. |
| first_indexed | 2024-12-10T05:51:36Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T05:51:36Z |
| publishDate | 2019-10-01 |
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| series | Molecules |
| spelling | doaj.art-e6135c683d9c404ba86b7e2f0ffe5acf2022-12-22T02:00:02ZengMDPI AGMolecules1420-30492019-10-012420368010.3390/molecules24203680molecules24203680Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i>Igor Khlusov0Elena Avdeeva1Valeria Shupletsova2Olga Khaziakhmatova3Larisa Litvinova4Ekaterina Porokhova5Yaroslav Reshetov6Irina Zvereva7Lyudmila Mushtovatova8Mariia Karpova9Artem Guryev10Irina Sukhodolo11Mikhail Belousov12Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, RussiaDepartment of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, RussiaBasic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaBasic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaBasic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaDepartment of Morphology and General Pathology, Siberian State Medical University, Tomsk 634050, RussiaDepartment of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, RussiaDepartment of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, RussiaDepartment of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, RussiaDepartment of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, RussiaDepartment of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, RussiaDepartment of Morphology and General Pathology, Siberian State Medical University, Tomsk 634050, RussiaResearch School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, RussiaThe osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of <i>Saussurea controversa</i> were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of <i>S. controversa</i> and have been characterized previously. PS-SC was first isolated from the leaves extract of <i>S. controversa</i> and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of <i>Staphylococcus aureus</i> strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10−50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of <i>S. controversa</i> and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.https://www.mdpi.com/1420-3049/24/20/3680<i>saussurea controversa</i> dc (asteraceae)polysaccharidesflavonoidshuman adipose-derived multipotent mesenchymal stromal cellscytotoxicityalizarin red staining<i>staphylococcus aureus</i> growth |
| spellingShingle | Igor Khlusov Elena Avdeeva Valeria Shupletsova Olga Khaziakhmatova Larisa Litvinova Ekaterina Porokhova Yaroslav Reshetov Irina Zvereva Lyudmila Mushtovatova Mariia Karpova Artem Guryev Irina Sukhodolo Mikhail Belousov Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i> Molecules <i>saussurea controversa</i> dc (asteraceae) polysaccharides flavonoids human adipose-derived multipotent mesenchymal stromal cells cytotoxicity alizarin red staining <i>staphylococcus aureus</i> growth |
| title | Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i> |
| title_full | Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i> |
| title_fullStr | Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i> |
| title_full_unstemmed | Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i> |
| title_short | Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i> |
| title_sort | comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of i saussurea controversa i |
| topic | <i>saussurea controversa</i> dc (asteraceae) polysaccharides flavonoids human adipose-derived multipotent mesenchymal stromal cells cytotoxicity alizarin red staining <i>staphylococcus aureus</i> growth |
| url | https://www.mdpi.com/1420-3049/24/20/3680 |
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