Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
Phospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position sn1 whereas the sn2-FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsatura...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/34394 |
| _version_ | 1818023843565928448 |
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| author | Marco M Manni Marion L Tiberti Sophie Pagnotta Hélène Barelli Romain Gautier Bruno Antonny |
| author_facet | Marco M Manni Marion L Tiberti Sophie Pagnotta Hélène Barelli Romain Gautier Bruno Antonny |
| author_sort | Marco M Manni |
| collection | DOAJ |
| description | Phospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position sn1 whereas the sn2-FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsaturation on membrane flexibility and fission is fragmentary. Here, we provide a comprehensive view of the effects of the FA profile of phospholipids on membrane vesiculation by dynamin and endophilin. Coupled to simulations, this analysis indicates that: (i) phospholipids with two polyunsaturated FAs make membranes prone to vesiculation but highly permeable; (ii) asymmetric sn1-saturated-sn2-polyunsaturated phospholipids provide a tradeoff between efficient membrane vesiculation and low membrane permeability; (iii) When incorporated into phospholipids, docosahexaenoic acid (DHA; omega-3) makes membranes more deformable than arachidonic acid (omega-6). These results suggest an explanation for the abundance of sn1-saturated-sn2-DHA phospholipids in synaptic membranes and for the importance of the omega-6/omega-3 ratio on neuronal functions. |
| first_indexed | 2024-12-10T03:50:46Z |
| format | Article |
| id | doaj.art-e613b9af60064d0b9cb5e79e77a18c06 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:50:46Z |
| publishDate | 2018-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e613b9af60064d0b9cb5e79e77a18c062022-12-22T02:03:16ZengeLife Sciences Publications LtdeLife2050-084X2018-03-01710.7554/eLife.34394Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakageMarco M Manni0Marion L Tiberti1Sophie Pagnotta2Hélène Barelli3Romain Gautier4Bruno Antonny5https://orcid.org/0000-0002-9166-8668Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur et CNRS, Valbonne, France; Instituto Biofisika (UPV/EHU, CSIC), Leioa, SpainInstitut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur et CNRS, Valbonne, FranceCentre Commun de Microscopie Appliquée, Université Côte d’Azur, Nice, FranceInstitut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur et CNRS, Valbonne, FranceInstitut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur et CNRS, Valbonne, FranceInstitut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur et CNRS, Valbonne, FrancePhospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position sn1 whereas the sn2-FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsaturation on membrane flexibility and fission is fragmentary. Here, we provide a comprehensive view of the effects of the FA profile of phospholipids on membrane vesiculation by dynamin and endophilin. Coupled to simulations, this analysis indicates that: (i) phospholipids with two polyunsaturated FAs make membranes prone to vesiculation but highly permeable; (ii) asymmetric sn1-saturated-sn2-polyunsaturated phospholipids provide a tradeoff between efficient membrane vesiculation and low membrane permeability; (iii) When incorporated into phospholipids, docosahexaenoic acid (DHA; omega-3) makes membranes more deformable than arachidonic acid (omega-6). These results suggest an explanation for the abundance of sn1-saturated-sn2-DHA phospholipids in synaptic membranes and for the importance of the omega-6/omega-3 ratio on neuronal functions.https://elifesciences.org/articles/34394polyunsaturated phospholipidDynaminmembrane curvaturemembrane fissionmembrane permeabilityomega-3 omega-6 PUFAs |
| spellingShingle | Marco M Manni Marion L Tiberti Sophie Pagnotta Hélène Barelli Romain Gautier Bruno Antonny Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage eLife polyunsaturated phospholipid Dynamin membrane curvature membrane fission membrane permeability omega-3 omega-6 PUFAs |
| title | Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage |
| title_full | Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage |
| title_fullStr | Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage |
| title_full_unstemmed | Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage |
| title_short | Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage |
| title_sort | acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage |
| topic | polyunsaturated phospholipid Dynamin membrane curvature membrane fission membrane permeability omega-3 omega-6 PUFAs |
| url | https://elifesciences.org/articles/34394 |
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