Using colony size to measure fitness in Saccharomyces cerevisiae.
Competitive fitness assays in liquid culture have been a mainstay for characterizing experimental evolution of microbial populations. Growth of microbial strains has also been extensively characterized by colony size and could serve as a useful alternative if translated to per generation measurement...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0271709 |
| _version_ | 1828070698997776384 |
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| author | James H Miller Vincent J Fasanello Ping Liu Emery R Longan Carlos A Botero Justin C Fay |
| author_facet | James H Miller Vincent J Fasanello Ping Liu Emery R Longan Carlos A Botero Justin C Fay |
| author_sort | James H Miller |
| collection | DOAJ |
| description | Competitive fitness assays in liquid culture have been a mainstay for characterizing experimental evolution of microbial populations. Growth of microbial strains has also been extensively characterized by colony size and could serve as a useful alternative if translated to per generation measurements of relative fitness. To examine fitness based on colony size, we established a relationship between cell number and colony size for strains of Saccharomyces cerevisiae robotically pinned onto solid agar plates in a high-density format. This was used to measure growth rates and estimate relative fitness differences between evolved strains and their ancestors. After controlling for edge effects through both normalization and agar-trimming, we found that colony size is a sensitive measure of fitness, capable of detecting 1% differences. While fitnesses determined from liquid and solid mediums were not equivalent, our results demonstrate that colony size provides a sensitive means of measuring fitness that is particularly well suited to measurements across many environments. |
| first_indexed | 2024-04-11T00:41:56Z |
| format | Article |
| id | doaj.art-e614b194ae374bb58515e662de0a688b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T00:41:56Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e614b194ae374bb58515e662de0a688b2023-01-06T05:30:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710e027170910.1371/journal.pone.0271709Using colony size to measure fitness in Saccharomyces cerevisiae.James H MillerVincent J FasanelloPing LiuEmery R LonganCarlos A BoteroJustin C FayCompetitive fitness assays in liquid culture have been a mainstay for characterizing experimental evolution of microbial populations. Growth of microbial strains has also been extensively characterized by colony size and could serve as a useful alternative if translated to per generation measurements of relative fitness. To examine fitness based on colony size, we established a relationship between cell number and colony size for strains of Saccharomyces cerevisiae robotically pinned onto solid agar plates in a high-density format. This was used to measure growth rates and estimate relative fitness differences between evolved strains and their ancestors. After controlling for edge effects through both normalization and agar-trimming, we found that colony size is a sensitive measure of fitness, capable of detecting 1% differences. While fitnesses determined from liquid and solid mediums were not equivalent, our results demonstrate that colony size provides a sensitive means of measuring fitness that is particularly well suited to measurements across many environments.https://doi.org/10.1371/journal.pone.0271709 |
| spellingShingle | James H Miller Vincent J Fasanello Ping Liu Emery R Longan Carlos A Botero Justin C Fay Using colony size to measure fitness in Saccharomyces cerevisiae. PLoS ONE |
| title | Using colony size to measure fitness in Saccharomyces cerevisiae. |
| title_full | Using colony size to measure fitness in Saccharomyces cerevisiae. |
| title_fullStr | Using colony size to measure fitness in Saccharomyces cerevisiae. |
| title_full_unstemmed | Using colony size to measure fitness in Saccharomyces cerevisiae. |
| title_short | Using colony size to measure fitness in Saccharomyces cerevisiae. |
| title_sort | using colony size to measure fitness in saccharomyces cerevisiae |
| url | https://doi.org/10.1371/journal.pone.0271709 |
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