Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels
Shiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-06-01
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| Series: | One Health |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771418300648 |
| _version_ | 1811324648894234624 |
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| author | Melinda Baschera Nicole Cernela Marc J.A. Stevens Anne Liljander Jörg Jores Victor Max Corman Magdalena Nüesch-Inderbinen Roger Stephan |
| author_facet | Melinda Baschera Nicole Cernela Marc J.A. Stevens Anne Liljander Jörg Jores Victor Max Corman Magdalena Nüesch-Inderbinen Roger Stephan |
| author_sort | Melinda Baschera |
| collection | DOAJ |
| description | Shiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter collected from 163 dromedaries on a large ranch were screened for the presence of stx1 and stx2. STEC strains were isolated and serotyped. Isolates were subjected to PCR for the subtyping of stx genes and for the detection of eae and ehx. In addition, whole genome sequencing (WGS) was carried out to detect further virulence genes and to determine the multilocus sequence types (MLST). Antimicrobial resistance profiles were determined by disk diffusion.STEC was isolated from 20 (12.3%) of the fecal samples. Thereof, nine (45%) isolates were STEC O156:H25, three (15%) isolates typed STEC O43:H2. The remaining isolates occurred as single serotypes or were O non-typeable. Eleven (55%) of the isolates harboured stx2a, nine (45%) eae, and 14 (70%) ehx, respectively. WGS revealed the presence of iss in 16 (80%), subAB in four (20%) and astA in two (10%) of the isolates, Furthermore, espA, tccP, nleA, nleB, tccP, and tir were found exclusively among STEC O156:H25.Eleven different sequence types (ST) were detected. The most prominent was ST300/ST5343, which comprised STEC O156:H25. All STEC isolates were pan susceptible to a panel of 16 antimicrobial agents. Overall, the results indicate that dromedary camels in Kenya may be reservoirs of STEC, including serotypes possessing virulence markers associated to disease in humans, such as STEC O156:H25. STEC in camels may represent a health hazard for humans with close contact to camels or to consumers of camel derived foodstuffs, such as unpasteurised camel milk. Keywords: Camels, STEC, Serotypes, Virulence genes, Whole genome sequencing |
| first_indexed | 2024-04-13T14:18:08Z |
| format | Article |
| id | doaj.art-e61a0c32a0564bf7a9154fe8b849efcb |
| institution | Directory Open Access Journal |
| issn | 2352-7714 |
| language | English |
| last_indexed | 2024-04-13T14:18:08Z |
| publishDate | 2019-06-01 |
| publisher | Elsevier |
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| series | One Health |
| spelling | doaj.art-e61a0c32a0564bf7a9154fe8b849efcb2022-12-22T02:43:35ZengElsevierOne Health2352-77142019-06-017Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camelsMelinda Baschera0Nicole Cernela1Marc J.A. Stevens2Anne Liljander3Jörg Jores4Victor Max Corman5Magdalena Nüesch-Inderbinen6Roger Stephan7Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, SwitzerlandInstitute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, SwitzerlandInstitute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, SwitzerlandInternational Livestock Research Institute, PO Box 30709, 00100 Nairobi, KenyaInstitute for Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, SwitzerlandCharité-Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Institute of Virology, Berlin, Germany; German Centre for Infection Research, Berlin, GermanyInstitute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Switzerland; Corresponding author at: Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, SwitzerlandShiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter collected from 163 dromedaries on a large ranch were screened for the presence of stx1 and stx2. STEC strains were isolated and serotyped. Isolates were subjected to PCR for the subtyping of stx genes and for the detection of eae and ehx. In addition, whole genome sequencing (WGS) was carried out to detect further virulence genes and to determine the multilocus sequence types (MLST). Antimicrobial resistance profiles were determined by disk diffusion.STEC was isolated from 20 (12.3%) of the fecal samples. Thereof, nine (45%) isolates were STEC O156:H25, three (15%) isolates typed STEC O43:H2. The remaining isolates occurred as single serotypes or were O non-typeable. Eleven (55%) of the isolates harboured stx2a, nine (45%) eae, and 14 (70%) ehx, respectively. WGS revealed the presence of iss in 16 (80%), subAB in four (20%) and astA in two (10%) of the isolates, Furthermore, espA, tccP, nleA, nleB, tccP, and tir were found exclusively among STEC O156:H25.Eleven different sequence types (ST) were detected. The most prominent was ST300/ST5343, which comprised STEC O156:H25. All STEC isolates were pan susceptible to a panel of 16 antimicrobial agents. Overall, the results indicate that dromedary camels in Kenya may be reservoirs of STEC, including serotypes possessing virulence markers associated to disease in humans, such as STEC O156:H25. STEC in camels may represent a health hazard for humans with close contact to camels or to consumers of camel derived foodstuffs, such as unpasteurised camel milk. Keywords: Camels, STEC, Serotypes, Virulence genes, Whole genome sequencinghttp://www.sciencedirect.com/science/article/pii/S2352771418300648 |
| spellingShingle | Melinda Baschera Nicole Cernela Marc J.A. Stevens Anne Liljander Jörg Jores Victor Max Corman Magdalena Nüesch-Inderbinen Roger Stephan Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels One Health |
| title | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_full | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_fullStr | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_full_unstemmed | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_short | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_sort | shiga toxin producing escherichia coli stec isolated from fecal samples of african dromedary camels |
| url | http://www.sciencedirect.com/science/article/pii/S2352771418300648 |
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