Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench)
Okra is widely used as vegetable, food ingredient, and traditional medicine in China for various applications. The main healthy properties are accepted to originate from the high polysaccharide content of okra. In this study, a homogenous pectic polysaccharide WOP-2 was extracted, isolated, and puri...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617307521 |
| _version_ | 1829508766662393856 |
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| author | Tao Zhang Jialin Xiang Guobo Zheng Renqing Yan Xun Min |
| author_facet | Tao Zhang Jialin Xiang Guobo Zheng Renqing Yan Xun Min |
| author_sort | Tao Zhang |
| collection | DOAJ |
| description | Okra is widely used as vegetable, food ingredient, and traditional medicine in China for various applications. The main healthy properties are accepted to originate from the high polysaccharide content of okra. In this study, a homogenous pectic polysaccharide WOP-2 was extracted, isolated, and purified from okra by DEAE-cellulose and Sepharose CL-6B column, and its molecular weight was estimated to be 580 kDa. Monosaccharide composition was analyzed to be Rha (21.4%), GalA (34.9%), Gal (29.6%), GlcA (4.5%), Glc (5.9%), and Ara (3.7%) by HPLC with PMP derivatization. Combined with NMR analysis, WOP-2 was elucidated to be a rhamnogalacturonan I backbone with type-II arabinogalactan side-chains substituted partly at O-4 of Rhap. WOP-2 exhibits significant anti-hyperglycemic activity on STZ-induced diabetic mice by inhibiting the lipid peroxidation chain reaction probably. Overall, these results provide new insight into structure–activity relationships of polysaccharide from okra and provide impetus towards the development of polysaccharide-based therapeutics on diabetes. |
| first_indexed | 2024-12-16T11:33:08Z |
| format | Article |
| id | doaj.art-e61af6adc84e4e53b82631f361e8cc97 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T11:33:08Z |
| publishDate | 2018-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e61af6adc84e4e53b82631f361e8cc972022-12-21T22:33:11ZengElsevierJournal of Functional Foods1756-46462018-02-01411924Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench)Tao Zhang0Jialin Xiang1Guobo Zheng2Renqing Yan3Xun Min4Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, PR China; Department of Laboratory Medicine, Zunyi Medical College, Zunyi 563000, PR ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, PR ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, PR China; Department of Laboratory Medicine, Zunyi Medical College, Zunyi 563000, PR ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, PR ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, PR China; Department of Laboratory Medicine, Zunyi Medical College, Zunyi 563000, PR China; Corresponding author at: Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, PR China.Okra is widely used as vegetable, food ingredient, and traditional medicine in China for various applications. The main healthy properties are accepted to originate from the high polysaccharide content of okra. In this study, a homogenous pectic polysaccharide WOP-2 was extracted, isolated, and purified from okra by DEAE-cellulose and Sepharose CL-6B column, and its molecular weight was estimated to be 580 kDa. Monosaccharide composition was analyzed to be Rha (21.4%), GalA (34.9%), Gal (29.6%), GlcA (4.5%), Glc (5.9%), and Ara (3.7%) by HPLC with PMP derivatization. Combined with NMR analysis, WOP-2 was elucidated to be a rhamnogalacturonan I backbone with type-II arabinogalactan side-chains substituted partly at O-4 of Rhap. WOP-2 exhibits significant anti-hyperglycemic activity on STZ-induced diabetic mice by inhibiting the lipid peroxidation chain reaction probably. Overall, these results provide new insight into structure–activity relationships of polysaccharide from okra and provide impetus towards the development of polysaccharide-based therapeutics on diabetes.http://www.sciencedirect.com/science/article/pii/S1756464617307521OkraPectic polysaccharideAnti-hyperglycemic activity |
| spellingShingle | Tao Zhang Jialin Xiang Guobo Zheng Renqing Yan Xun Min Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench) Journal of Functional Foods Okra Pectic polysaccharide Anti-hyperglycemic activity |
| title | Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench) |
| title_full | Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench) |
| title_fullStr | Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench) |
| title_full_unstemmed | Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench) |
| title_short | Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench) |
| title_sort | preliminary characterization and anti hyperglycemic activity of a pectic polysaccharide from okra abelmoschus esculentus l moench |
| topic | Okra Pectic polysaccharide Anti-hyperglycemic activity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617307521 |
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