Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity
This work is the first attempt to reveal the chemical compositions, as well as the relationships between the chemical compositions and anti-fatigue effect of Oviductus Ranae (OR) and Rana chensinensis ovum (RCO). In this work, an integrated strategy was applied containing comprehensive chemical prof...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623002803 |
| _version_ | 1827871793721901056 |
|---|---|
| author | Zhongyao Wang Yunhe Liu Yue Zhou Shihan Wang Xinxin Xu Xuanrui Fan Miao Chen Yong Yang Zhihan Wang Yongsheng Wang |
| author_facet | Zhongyao Wang Yunhe Liu Yue Zhou Shihan Wang Xinxin Xu Xuanrui Fan Miao Chen Yong Yang Zhihan Wang Yongsheng Wang |
| author_sort | Zhongyao Wang |
| collection | DOAJ |
| description | This work is the first attempt to reveal the chemical compositions, as well as the relationships between the chemical compositions and anti-fatigue effect of Oviductus Ranae (OR) and Rana chensinensis ovum (RCO). In this work, an integrated strategy was applied containing comprehensive chemical profiling analysis, extraction methods, in vivo study, multivariate statistics, and network pharmacology. Here, 138 compounds were identified or briefly characterized using UPLC-Q/TOF-MS. About 24 and 35 variables were considered to be discriminating compounds from petroleum ether and ethanol extracts of OR and RCO, respectively. In addition, 41 identified compounds in OR and RCO were significantly correlated with anti-fatigue parameters. Finally, 22 candidate markers in OR and RCO were screened. Network pharmacology analysis was employed to verify those markers and screen the potential targets for anti-fatigue effect. Screened targets were further validated using RT-PCR. In muscle tissues, AKT mRNA, PI3K mRNA, and mTOR mRNA were upregulated following OR and RCO administration. This work not only paved the way for further study of OR and RCO in anti-fatigue function, but also provided essential information for their products quality control. |
| first_indexed | 2024-03-12T16:15:55Z |
| format | Article |
| id | doaj.art-e61c1581fd5d4191bfbb8fa2ae907e57 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:55Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e61c1581fd5d4191bfbb8fa2ae907e572023-08-09T04:32:34ZengElsevierJournal of Functional Foods1756-46462023-08-01107105680Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activityZhongyao Wang0Yunhe Liu1Yue Zhou2Shihan Wang3Xinxin Xu4Xuanrui Fan5Miao Chen6Yong Yang7Zhihan Wang8Yongsheng Wang9School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, Jilin 130118, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, China; Corresponding authors.School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, China; Corresponding authors.This work is the first attempt to reveal the chemical compositions, as well as the relationships between the chemical compositions and anti-fatigue effect of Oviductus Ranae (OR) and Rana chensinensis ovum (RCO). In this work, an integrated strategy was applied containing comprehensive chemical profiling analysis, extraction methods, in vivo study, multivariate statistics, and network pharmacology. Here, 138 compounds were identified or briefly characterized using UPLC-Q/TOF-MS. About 24 and 35 variables were considered to be discriminating compounds from petroleum ether and ethanol extracts of OR and RCO, respectively. In addition, 41 identified compounds in OR and RCO were significantly correlated with anti-fatigue parameters. Finally, 22 candidate markers in OR and RCO were screened. Network pharmacology analysis was employed to verify those markers and screen the potential targets for anti-fatigue effect. Screened targets were further validated using RT-PCR. In muscle tissues, AKT mRNA, PI3K mRNA, and mTOR mRNA were upregulated following OR and RCO administration. This work not only paved the way for further study of OR and RCO in anti-fatigue function, but also provided essential information for their products quality control.http://www.sciencedirect.com/science/article/pii/S1756464623002803Oviductus RanaeRana chensinensis ovumChemical compositonActivity marker |
| spellingShingle | Zhongyao Wang Yunhe Liu Yue Zhou Shihan Wang Xinxin Xu Xuanrui Fan Miao Chen Yong Yang Zhihan Wang Yongsheng Wang Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity Journal of Functional Foods Oviductus Ranae Rana chensinensis ovum Chemical compositon Activity marker |
| title | Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity |
| title_full | Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity |
| title_fullStr | Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity |
| title_full_unstemmed | Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity |
| title_short | Integrated strategy for anti-fatigue markers screening of gonads of Rana temporaria chensinensis David based on comprehensive chemical analysis and in vivo activity |
| title_sort | integrated strategy for anti fatigue markers screening of gonads of rana temporaria chensinensis david based on comprehensive chemical analysis and in vivo activity |
| topic | Oviductus Ranae Rana chensinensis ovum Chemical compositon Activity marker |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623002803 |
| work_keys_str_mv | AT zhongyaowang integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT yunheliu integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT yuezhou integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT shihanwang integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT xinxinxu integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT xuanruifan integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT miaochen integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT yongyang integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT zhihanwang integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity AT yongshengwang integratedstrategyforantifatiguemarkersscreeningofgonadsofranatemporariachensinensisdavidbasedoncomprehensivechemicalanalysisandinvivoactivity |