Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose
Regulations often are imposing long postmortem times before autopsy leading to certain toxicity-unrelated changes in biomarkers, which in turn may affect the reliability of toxicity evaluation during forensic investigations. Since methomyl pesticide shows significant toxicity and is frequently encou...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022001822 |
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| author | Shreen D. Nusair Mohammad Ananbeh Aref Zayed Mohammad I. Ahmad Nidal A. Qinna |
| author_facet | Shreen D. Nusair Mohammad Ananbeh Aref Zayed Mohammad I. Ahmad Nidal A. Qinna |
| author_sort | Shreen D. Nusair |
| collection | DOAJ |
| description | Regulations often are imposing long postmortem times before autopsy leading to certain toxicity-unrelated changes in biomarkers, which in turn may affect the reliability of toxicity evaluation during forensic investigations. Since methomyl pesticide shows significant toxicity and is frequently encountered in poisoning cases, the current study evaluated different parameters in methomyl intoxicated rats at three different postmortem intervals (Hour 0, Hour 3 and Hour 6). Eighteen adult Sprague Dawley rats were poisoned with methomyl to simulate actual methomyl poisoning cases. The time of death was assigned as Hour 0. The animals were divided into 3 groups (n = 6) to collect blood and tissue samples at the selected time points. Body weight, relative organ weight, protein concentration, methomyl concentration and acetylcholinesterase activity (AChE) were assessed in blood and different tissues (liver, spleen, kidney, brain, eye, and bone marrow) to evaluate the effect of postmortem sampling time. Outcomes revealed significant decreases in methomyl concentration in blood and bone marrow with advanced sampling time (P < 0.001). Similarly, there were significant reductions in AChE activity in the kidney (P < 0.01), while the enzyme activity significantly increased in brain samples (P < 0.05). Findings illustrated the importance of sampling time in toxicity studies because it could alter experimental results and impact consequent interpretations, as well as it may alter postmortem biomarkers in related forensic cases. |
| first_indexed | 2024-04-12T02:31:29Z |
| format | Article |
| id | doaj.art-e61d64f7ed374ff1b491c6c0110a60a8 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-12T02:31:29Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
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| series | Toxicology Reports |
| spelling | doaj.art-e61d64f7ed374ff1b491c6c0110a60a82022-12-22T03:51:45ZengElsevierToxicology Reports2214-75002022-01-01916741680Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl doseShreen D. Nusair0Mohammad Ananbeh1Aref Zayed2Mohammad I. Ahmad3Nidal A. Qinna4Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan; Correspondence to: Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, P.O.Box 3030, Irbid 22110, Jordan.Department of Forensic Medicine Toxicology and Forensic Sciences, Faculty of Medicine, Jordan University of Science and Technology, Irbid, JordanDepartment of Medicinal Chemistry and Phramacognosy, Jordan University of Science and Technology, Irbid, JordanUniversity of Petra Pharmaceutical Center (UPPC), Faculty of Pharmacy & Medical Sciences, University of Petra, Amman, JordanUniversity of Petra Pharmaceutical Center (UPPC), Faculty of Pharmacy & Medical Sciences, University of Petra, Amman, JordanRegulations often are imposing long postmortem times before autopsy leading to certain toxicity-unrelated changes in biomarkers, which in turn may affect the reliability of toxicity evaluation during forensic investigations. Since methomyl pesticide shows significant toxicity and is frequently encountered in poisoning cases, the current study evaluated different parameters in methomyl intoxicated rats at three different postmortem intervals (Hour 0, Hour 3 and Hour 6). Eighteen adult Sprague Dawley rats were poisoned with methomyl to simulate actual methomyl poisoning cases. The time of death was assigned as Hour 0. The animals were divided into 3 groups (n = 6) to collect blood and tissue samples at the selected time points. Body weight, relative organ weight, protein concentration, methomyl concentration and acetylcholinesterase activity (AChE) were assessed in blood and different tissues (liver, spleen, kidney, brain, eye, and bone marrow) to evaluate the effect of postmortem sampling time. Outcomes revealed significant decreases in methomyl concentration in blood and bone marrow with advanced sampling time (P < 0.001). Similarly, there were significant reductions in AChE activity in the kidney (P < 0.01), while the enzyme activity significantly increased in brain samples (P < 0.05). Findings illustrated the importance of sampling time in toxicity studies because it could alter experimental results and impact consequent interpretations, as well as it may alter postmortem biomarkers in related forensic cases.http://www.sciencedirect.com/science/article/pii/S2214750022001822PesticidesMethomylToxicityLC-MS/MSSPEAcetylcholinesterase, Sampling time |
| spellingShingle | Shreen D. Nusair Mohammad Ananbeh Aref Zayed Mohammad I. Ahmad Nidal A. Qinna Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose Toxicology Reports Pesticides Methomyl Toxicity LC-MS/MS SPE Acetylcholinesterase, Sampling time |
| title | Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose |
| title_full | Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose |
| title_fullStr | Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose |
| title_full_unstemmed | Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose |
| title_short | Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose |
| title_sort | postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose |
| topic | Pesticides Methomyl Toxicity LC-MS/MS SPE Acetylcholinesterase, Sampling time |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022001822 |
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