Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala
The basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specifi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/59003 |
| _version_ | 1811199347961888768 |
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| author | Timothy P O'Leary Kaitlin E Sullivan Lihua Wang Jody Clements Andrew L Lemire Mark S Cembrowski |
| author_facet | Timothy P O'Leary Kaitlin E Sullivan Lihua Wang Jody Clements Andrew L Lemire Mark S Cembrowski |
| author_sort | Timothy P O'Leary |
| collection | DOAJ |
| description | The basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specific landscape of the BLA. Here, beginning with single-cell RNA sequencing, we identified both discrete and graded continuous gene-expression differences within the mouse BLA. Via in situ hybridization, we next mapped this discrete transcriptomic heterogeneity onto a sharp spatial border between the basal and lateral amygdala nuclei, and identified continuous spatial gene-expression gradients within each of these regions. These discrete and continuous spatial transformations of transcriptomic cell-type identity were recapitulated by local morphology as well as long-range connectivity. Thus, BLA excitatory neurons are a highly heterogenous collection of neurons that spatially covary in molecular, cellular, and circuit properties. This heterogeneity likely drives pronounced spatial variation in BLA computation and function. |
| first_indexed | 2024-04-12T01:46:36Z |
| format | Article |
| id | doaj.art-e623468e4b80415995274634a674b41a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:46:36Z |
| publishDate | 2020-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e623468e4b80415995274634a674b41a2022-12-22T03:53:03ZengeLife Sciences Publications LtdeLife2050-084X2020-09-01910.7554/eLife.59003Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdalaTimothy P O'Leary0Kaitlin E Sullivan1https://orcid.org/0000-0001-8043-7111Lihua Wang2Jody Clements3Andrew L Lemire4Mark S Cembrowski5https://orcid.org/0000-0001-8275-7362Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, CanadaJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada; School of Biomedical Engineering, University of British Columbia, Vancouver, CanadaThe basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specific landscape of the BLA. Here, beginning with single-cell RNA sequencing, we identified both discrete and graded continuous gene-expression differences within the mouse BLA. Via in situ hybridization, we next mapped this discrete transcriptomic heterogeneity onto a sharp spatial border between the basal and lateral amygdala nuclei, and identified continuous spatial gene-expression gradients within each of these regions. These discrete and continuous spatial transformations of transcriptomic cell-type identity were recapitulated by local morphology as well as long-range connectivity. Thus, BLA excitatory neurons are a highly heterogenous collection of neurons that spatially covary in molecular, cellular, and circuit properties. This heterogeneity likely drives pronounced spatial variation in BLA computation and function.https://elifesciences.org/articles/59003amygdalatranscriptomicscell typeneural circuits |
| spellingShingle | Timothy P O'Leary Kaitlin E Sullivan Lihua Wang Jody Clements Andrew L Lemire Mark S Cembrowski Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala eLife amygdala transcriptomics cell type neural circuits |
| title | Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala |
| title_full | Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala |
| title_fullStr | Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala |
| title_full_unstemmed | Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala |
| title_short | Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala |
| title_sort | extensive and spatially variable within cell type heterogeneity across the basolateral amygdala |
| topic | amygdala transcriptomics cell type neural circuits |
| url | https://elifesciences.org/articles/59003 |
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