Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.
The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen - 17beta-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5th-15th postnatal days...
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| Format: | Article |
| Language: | English |
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Via Medica
2010-01-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4288 |
| _version_ | 1811301153466482688 |
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| author | Katarzyna Marchlewska Anna Guminska Elzbieta Oszukowska Renata Walczak-Jedrzejowska Eliza Filipiak Krzysztof Kula Jolanta Slowikowska-Hilczer |
| author_facet | Katarzyna Marchlewska Anna Guminska Elzbieta Oszukowska Renata Walczak-Jedrzejowska Eliza Filipiak Krzysztof Kula Jolanta Slowikowska-Hilczer |
| author_sort | Katarzyna Marchlewska |
| collection | DOAJ |
| description | The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen - 17beta-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5th-15th postnatal days (p.d.) with E (1.25 or 12.5 mug) or DES (1.25 or 12.5 mug) or ZEA (4 or 40 mug) or vehicle. At 16th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B), preleptotene spermatocytes (PL), leptotene+zygotene+pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. Conclusion: exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens. |
| first_indexed | 2024-04-13T07:03:50Z |
| format | Article |
| id | doaj.art-e6252dd96500432fb4bd5a37d22df2d4 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-04-13T07:03:50Z |
| publishDate | 2010-01-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-e6252dd96500432fb4bd5a37d22df2d42022-12-22T02:57:03ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312010-01-0147511312010.5603/4288Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.Katarzyna MarchlewskaAnna GuminskaElzbieta OszukowskaRenata Walczak-JedrzejowskaEliza FilipiakKrzysztof KulaJolanta Slowikowska-HilczerThe aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen - 17beta-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5th-15th postnatal days (p.d.) with E (1.25 or 12.5 mug) or DES (1.25 or 12.5 mug) or ZEA (4 or 40 mug) or vehicle. At 16th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B), preleptotene spermatocytes (PL), leptotene+zygotene+pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. Conclusion: exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens.http://czasopisma.viamedica.pl/fhc/article/view/4288 |
| spellingShingle | Katarzyna Marchlewska Anna Guminska Elzbieta Oszukowska Renata Walczak-Jedrzejowska Eliza Filipiak Krzysztof Kula Jolanta Slowikowska-Hilczer Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis. Folia Histochemica et Cytobiologica |
| title | Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis. |
| title_full | Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis. |
| title_fullStr | Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis. |
| title_full_unstemmed | Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis. |
| title_short | Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis. |
| title_sort | xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat s testis |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4288 |
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