Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length
The primary domain/leucine zipper (bZIP) transcription factor, Elongated Hypocotyl (HY5), is crucial for the photomorphogenesis of seedlings. Here, we identified <i>BcHY5</i> as a regulator of hypocotyl length from the non-heading Chinese cabbage (NHCC) variety ‘Suzhouqing’, which encode...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/10/2573 |
| _version_ | 1797476089428180992 |
|---|---|
| author | Yiran Li Ying He Wenyuan Lin Cheng Jiang Xilin Hou |
| author_facet | Yiran Li Ying He Wenyuan Lin Cheng Jiang Xilin Hou |
| author_sort | Yiran Li |
| collection | DOAJ |
| description | The primary domain/leucine zipper (bZIP) transcription factor, Elongated Hypocotyl (HY5), is crucial for the photomorphogenesis of seedlings. Here, we identified <i>BcHY5</i> as a regulator of hypocotyl length from the non-heading Chinese cabbage (NHCC) variety ‘Suzhouqing’, which encoded a protein comprised 164 amino acid residues. Ectopic expression of <i>BcHY5</i> in <i>Arabidopsis</i> shortens the length of the hypocotyl. Additionally, we discovered a protein called BcBBX24 containing the B-BOX (BBX) domain, which is the interacting partner of BcHY5. Yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and GST pull-down assays revealed that BcHY5 interacted with BcBBX24. Additionally, by physically binding to the promoter of <i>BcHY5</i>, BcBBX24 inhibited the transcriptional activity of BcHY5. Therefore, our findings reveal a transcriptional mechanism through which light response occurs in NHCC seedlings, where BcHY5 can interact with BcBBX24 and BcBBX24 can prevent <i>BcHY5</i> transcription. |
| first_indexed | 2024-03-09T20:53:02Z |
| format | Article |
| id | doaj.art-e627f0a0b84749fb98e309c6283da581 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:53:02Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-e627f0a0b84749fb98e309c6283da5812023-11-23T22:29:30ZengMDPI AGAgronomy2073-43952022-10-011210257310.3390/agronomy12102573Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl LengthYiran Li0Ying He1Wenyuan Lin2Cheng Jiang3Xilin Hou4State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Nanjing Agricultural University, Nanjing 210095, ChinaThe primary domain/leucine zipper (bZIP) transcription factor, Elongated Hypocotyl (HY5), is crucial for the photomorphogenesis of seedlings. Here, we identified <i>BcHY5</i> as a regulator of hypocotyl length from the non-heading Chinese cabbage (NHCC) variety ‘Suzhouqing’, which encoded a protein comprised 164 amino acid residues. Ectopic expression of <i>BcHY5</i> in <i>Arabidopsis</i> shortens the length of the hypocotyl. Additionally, we discovered a protein called BcBBX24 containing the B-BOX (BBX) domain, which is the interacting partner of BcHY5. Yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and GST pull-down assays revealed that BcHY5 interacted with BcBBX24. Additionally, by physically binding to the promoter of <i>BcHY5</i>, BcBBX24 inhibited the transcriptional activity of BcHY5. Therefore, our findings reveal a transcriptional mechanism through which light response occurs in NHCC seedlings, where BcHY5 can interact with BcBBX24 and BcBBX24 can prevent <i>BcHY5</i> transcription.https://www.mdpi.com/2073-4395/12/10/2573non-heading Chinese cabbage<i>BcHY5</i>photomorphogenesishypocotyl<i>BcBBX24</i> |
| spellingShingle | Yiran Li Ying He Wenyuan Lin Cheng Jiang Xilin Hou Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length Agronomy non-heading Chinese cabbage <i>BcHY5</i> photomorphogenesis hypocotyl <i>BcBBX24</i> |
| title | Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length |
| title_full | Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length |
| title_fullStr | Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length |
| title_full_unstemmed | Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length |
| title_short | Identification of the <i>Brassica</i> <i>Campestris</i> ssp. <i>Chinensis</i> <i>BcHY5</i> Gene Important for Hypocotyl Length |
| title_sort | identification of the i brassica i i campestris i ssp i chinensis i i bchy5 i gene important for hypocotyl length |
| topic | non-heading Chinese cabbage <i>BcHY5</i> photomorphogenesis hypocotyl <i>BcBBX24</i> |
| url | https://www.mdpi.com/2073-4395/12/10/2573 |
| work_keys_str_mv | AT yiranli identificationoftheibrassicaiicampestrisisspichinensisiibchy5igeneimportantforhypocotyllength AT yinghe identificationoftheibrassicaiicampestrisisspichinensisiibchy5igeneimportantforhypocotyllength AT wenyuanlin identificationoftheibrassicaiicampestrisisspichinensisiibchy5igeneimportantforhypocotyllength AT chengjiang identificationoftheibrassicaiicampestrisisspichinensisiibchy5igeneimportantforhypocotyllength AT xilinhou identificationoftheibrassicaiicampestrisisspichinensisiibchy5igeneimportantforhypocotyllength |