Molecular detection of Acute Bee Paralysis Virus in Iran

Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortali...

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Main Authors: Moharrami, M., Modirrousta, H.
Format: Article
Language:English
Published: Razi Vaccine and Serum Research Institute 2013-12-01
Series:Archives of Razi Institute
Subjects:
Online Access:http://www.archrazi.com/browse.php?a_id=413&sid=1&slc_lang=en
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author Moharrami, M.
Modirrousta, H.
author_facet Moharrami, M.
Modirrousta, H.
author_sort Moharrami, M.
collection DOAJ
description Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. The aim of this study was evaluation of ABPV infection in honey bee colonies in apiaries with loss of population in different geographical provinces in Iran. Adult bee samples were collected between July - September 2011 and 2012 and were originated from 23 provinces with different geographic areas of Iran. Following the RT-PCR reaction with the specific primers on the isolated RNA, an approximately 618 bp product was detected. We demonstrated the presence of ABPV RNA in 9 (5.62 %) out of 160 samples collected from Iranian apiaries.
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spelling doaj.art-e62835ccaf7b4f90985591fa1367bd212022-12-22T01:49:26ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722013-12-01682101104Molecular detection of Acute Bee Paralysis Virus in IranMoharrami, M.Modirrousta, H.Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. The aim of this study was evaluation of ABPV infection in honey bee colonies in apiaries with loss of population in different geographical provinces in Iran. Adult bee samples were collected between July - September 2011 and 2012 and were originated from 23 provinces with different geographic areas of Iran. Following the RT-PCR reaction with the specific primers on the isolated RNA, an approximately 618 bp product was detected. We demonstrated the presence of ABPV RNA in 9 (5.62 %) out of 160 samples collected from Iranian apiaries.http://www.archrazi.com/browse.php?a_id=413&sid=1&slc_lang=enAcute Bee ParalysisVirusRT-PCRHoney beeIran
spellingShingle Moharrami, M.
Modirrousta, H.
Molecular detection of Acute Bee Paralysis Virus in Iran
Archives of Razi Institute
Acute Bee Paralysis
Virus
RT-PCR
Honey bee
Iran
title Molecular detection of Acute Bee Paralysis Virus in Iran
title_full Molecular detection of Acute Bee Paralysis Virus in Iran
title_fullStr Molecular detection of Acute Bee Paralysis Virus in Iran
title_full_unstemmed Molecular detection of Acute Bee Paralysis Virus in Iran
title_short Molecular detection of Acute Bee Paralysis Virus in Iran
title_sort molecular detection of acute bee paralysis virus in iran
topic Acute Bee Paralysis
Virus
RT-PCR
Honey bee
Iran
url http://www.archrazi.com/browse.php?a_id=413&sid=1&slc_lang=en
work_keys_str_mv AT moharramim moleculardetectionofacutebeeparalysisvirusiniran
AT modirroustah moleculardetectionofacutebeeparalysisvirusiniran