Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing
Wounds represent a major global health challenge. Acute and chronic wounds are sensitive to bacterial infection. The wound environment facilitates the development of microbial biofilms, delays healing, and promotes chronic inflammation processes. The aim of the present work is the development of chi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/7757 |
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| author | Carla Russo Miranda Piccioni Maria Laura Lorenzini Chiara Catalano Valeria Ambrogi Rita Pagiotti Donatella Pietrella |
| author_facet | Carla Russo Miranda Piccioni Maria Laura Lorenzini Chiara Catalano Valeria Ambrogi Rita Pagiotti Donatella Pietrella |
| author_sort | Carla Russo |
| collection | DOAJ |
| description | Wounds represent a major global health challenge. Acute and chronic wounds are sensitive to bacterial infection. The wound environment facilitates the development of microbial biofilms, delays healing, and promotes chronic inflammation processes. The aim of the present work is the development of chitosan films embedded with bud poplar extract (BPE) to be used as wound dressing for avoiding biofilm formation and healing delay. Chitosan is a polymer with antimicrobial and hydrating properties used in wound dressing, while BPE has antibacterial, antioxidative, and anti-inflammatory properties. Chitosan-BPE films showed good antimicrobial and antibiofilm properties against Gram-positive bacteria and the yeast <i>Candida albicans</i>. BPE extract induced an immunomodulatory effect on human macrophages, increasing CD36 expression and TGFβ production during M1/M2 polarization, as observed by means of cytofluorimetric analysis and ELISA assay. Significant antioxidant activity was revealed in a cell-free test and in a human neutrophil assay. Moreover, the chitosan-BPE films induced a good regenerative effect in human fibroblasts by in vitro cell migration assay. Our results suggest that chitosan-BPE films could be considered a valid plant-based antimicrobial material for advanced dressings focused on the acceleration of wound repair. |
| first_indexed | 2024-03-09T18:08:23Z |
| format | Article |
| id | doaj.art-e6289fb1cb3b4c2aafffce3cb11d636c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:08:23Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e6289fb1cb3b4c2aafffce3cb11d636c2023-11-24T09:20:38ZengMDPI AGMolecules1420-30492022-11-012722775710.3390/molecules27227757Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing DressingCarla Russo0Miranda Piccioni1Maria Laura Lorenzini2Chiara Catalano3Valeria Ambrogi4Rita Pagiotti5Donatella Pietrella6Medical Microbiology Unit, Department of Medicine and Surgery, University of Perugia, Piazzale Sereni, Building D, 4th Floor, 06129 Perugia, ItalyBiochemical Sciences and Health Unit, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, ItalyPharmaceutical Technology Unit, Department of Pharmaceutical Sciences, University of Perugia, Via del Liceo 1, 06123 Perugia, ItalyBiochemical Sciences and Health Unit, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, ItalyPharmaceutical Technology Unit, Department of Pharmaceutical Sciences, University of Perugia, Via del Liceo 1, 06123 Perugia, ItalyBiochemical Sciences and Health Unit, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, ItalyMedical Microbiology Unit, Department of Medicine and Surgery, University of Perugia, Piazzale Sereni, Building D, 4th Floor, 06129 Perugia, ItalyWounds represent a major global health challenge. Acute and chronic wounds are sensitive to bacterial infection. The wound environment facilitates the development of microbial biofilms, delays healing, and promotes chronic inflammation processes. The aim of the present work is the development of chitosan films embedded with bud poplar extract (BPE) to be used as wound dressing for avoiding biofilm formation and healing delay. Chitosan is a polymer with antimicrobial and hydrating properties used in wound dressing, while BPE has antibacterial, antioxidative, and anti-inflammatory properties. Chitosan-BPE films showed good antimicrobial and antibiofilm properties against Gram-positive bacteria and the yeast <i>Candida albicans</i>. BPE extract induced an immunomodulatory effect on human macrophages, increasing CD36 expression and TGFβ production during M1/M2 polarization, as observed by means of cytofluorimetric analysis and ELISA assay. Significant antioxidant activity was revealed in a cell-free test and in a human neutrophil assay. Moreover, the chitosan-BPE films induced a good regenerative effect in human fibroblasts by in vitro cell migration assay. Our results suggest that chitosan-BPE films could be considered a valid plant-based antimicrobial material for advanced dressings focused on the acceleration of wound repair.https://www.mdpi.com/1420-3049/27/22/7757bud poplar resin extractchitosanwound healingantibiofilm activityantioxidant activityanti-inflammatory activity |
| spellingShingle | Carla Russo Miranda Piccioni Maria Laura Lorenzini Chiara Catalano Valeria Ambrogi Rita Pagiotti Donatella Pietrella Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing Molecules bud poplar resin extract chitosan wound healing antibiofilm activity antioxidant activity anti-inflammatory activity |
| title | Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing |
| title_full | Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing |
| title_fullStr | Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing |
| title_full_unstemmed | Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing |
| title_short | Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing |
| title_sort | bud poplar extract embedded chitosan films as multifunctional wound healing dressing |
| topic | bud poplar resin extract chitosan wound healing antibiofilm activity antioxidant activity anti-inflammatory activity |
| url | https://www.mdpi.com/1420-3049/27/22/7757 |
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