Study of the gut microbiome in Egyptian patients with Parkinson’s Disease
Abstract Background Recently, an important relationship between Parkinson’s disease and the gut microbiota, through the brain-gut axis interactions, has been established. Previous studies have declared that alterations in the gut microbiota have a great impact on the pathogenesis and clinical pictur...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02933-7 |
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| author | Mohammad Mehanna Suzan AbuRaya Shwikar Mahmoud Ahmed Ghada Ashmawy Ahmed Ibrahim Essameldin AbdelKhaliq |
| author_facet | Mohammad Mehanna Suzan AbuRaya Shwikar Mahmoud Ahmed Ghada Ashmawy Ahmed Ibrahim Essameldin AbdelKhaliq |
| author_sort | Mohammad Mehanna |
| collection | DOAJ |
| description | Abstract Background Recently, an important relationship between Parkinson’s disease and the gut microbiota, through the brain-gut axis interactions, has been established. Previous studies have declared that alterations in the gut microbiota have a great impact on the pathogenesis and clinical picture of Parkinson’s disease (PD). The present study aimed to identify the gut microbiome that is likely related to Parkinson’s disease as well as their possible relation to clinical phenotypes. Methods Thirty patients with Parkinson’s disease, who presented to the Parkinson’s disease Neurology Clinic of Alexandria University Hospital were enrolled in our study. A cross-matching control group of 35 healthy subjects of similar age and sex were included. Stool specimens were taken from each. Quantitative SYBR Green Real-Time PCR was done for the identification and quantitation of selected bacterial phyla, genera and/or species. Results There was a significant increase in Bacteroides and a significant decrease of Firmicutes and Firmicutes / Bacteroidetes ratio and Bifidobacteria in PD patients. Although Prevotella was decreased among PD patients relative to the healthy control, the difference was not statistically significant. Comparing the PD clinical phenotypes with the control group, the Mixed phenotype had significantly higher Bacteroides, Tremors predominant had lower Firmicutes and Firmicutes / Bacteroidetes ratio, and both tremors and postural instability and gait disability (PIGD) phenotypes had lower Bifidobacteria. However, there was no statistically significant difference between these phenotypes. Furthermore, when comparing tremors and non-tremors predominant phenotypes; Lactobacilli showed a significant decrease in non-tremors predominant phenotypes. Conclusions The current study showed evidence of changes in the gut microbiome of Parkinson’s disease patients compared to the healthy controls. These observations may highlight the importance of the identification of microbiome and specific bacterial changes that can be targeted for the treatment of Parkinson’s disease. |
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| format | Article |
| id | doaj.art-e62b23ad14e14d4d8ec6f2920e3a45e6 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2025-03-21T09:41:38Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-e62b23ad14e14d4d8ec6f2920e3a45e62024-07-07T11:12:38ZengBMCBMC Microbiology1471-21802023-07-0123111110.1186/s12866-023-02933-7Study of the gut microbiome in Egyptian patients with Parkinson’s DiseaseMohammad Mehanna0Suzan AbuRaya1Shwikar Mahmoud Ahmed2Ghada Ashmawy3Ahmed Ibrahim4Essameldin AbdelKhaliq5Internal Medicine, Faculty of Medicine, University of AlexandriaInternal Medicine, Faculty of Medicine, University of AlexandriaMedical Microbiology and Immunology Department, Faculty of Medicine, University of AlexandriaDepartment of Neuropsychiatry, Faculty of Medicine, University of AlexandriaDepartment of Neuropsychiatry, Faculty of Medicine, University of AlexandriaInternal Medicine, Faculty of Medicine, University of AlexandriaAbstract Background Recently, an important relationship between Parkinson’s disease and the gut microbiota, through the brain-gut axis interactions, has been established. Previous studies have declared that alterations in the gut microbiota have a great impact on the pathogenesis and clinical picture of Parkinson’s disease (PD). The present study aimed to identify the gut microbiome that is likely related to Parkinson’s disease as well as their possible relation to clinical phenotypes. Methods Thirty patients with Parkinson’s disease, who presented to the Parkinson’s disease Neurology Clinic of Alexandria University Hospital were enrolled in our study. A cross-matching control group of 35 healthy subjects of similar age and sex were included. Stool specimens were taken from each. Quantitative SYBR Green Real-Time PCR was done for the identification and quantitation of selected bacterial phyla, genera and/or species. Results There was a significant increase in Bacteroides and a significant decrease of Firmicutes and Firmicutes / Bacteroidetes ratio and Bifidobacteria in PD patients. Although Prevotella was decreased among PD patients relative to the healthy control, the difference was not statistically significant. Comparing the PD clinical phenotypes with the control group, the Mixed phenotype had significantly higher Bacteroides, Tremors predominant had lower Firmicutes and Firmicutes / Bacteroidetes ratio, and both tremors and postural instability and gait disability (PIGD) phenotypes had lower Bifidobacteria. However, there was no statistically significant difference between these phenotypes. Furthermore, when comparing tremors and non-tremors predominant phenotypes; Lactobacilli showed a significant decrease in non-tremors predominant phenotypes. Conclusions The current study showed evidence of changes in the gut microbiome of Parkinson’s disease patients compared to the healthy controls. These observations may highlight the importance of the identification of microbiome and specific bacterial changes that can be targeted for the treatment of Parkinson’s disease.https://doi.org/10.1186/s12866-023-02933-7Parkinson’s diseaseGut microbiomeBrain-gut axisReal-time PCR16S rRNADysbiosis |
| spellingShingle | Mohammad Mehanna Suzan AbuRaya Shwikar Mahmoud Ahmed Ghada Ashmawy Ahmed Ibrahim Essameldin AbdelKhaliq Study of the gut microbiome in Egyptian patients with Parkinson’s Disease BMC Microbiology Parkinson’s disease Gut microbiome Brain-gut axis Real-time PCR 16S rRNA Dysbiosis |
| title | Study of the gut microbiome in Egyptian patients with Parkinson’s Disease |
| title_full | Study of the gut microbiome in Egyptian patients with Parkinson’s Disease |
| title_fullStr | Study of the gut microbiome in Egyptian patients with Parkinson’s Disease |
| title_full_unstemmed | Study of the gut microbiome in Egyptian patients with Parkinson’s Disease |
| title_short | Study of the gut microbiome in Egyptian patients with Parkinson’s Disease |
| title_sort | study of the gut microbiome in egyptian patients with parkinson s disease |
| topic | Parkinson’s disease Gut microbiome Brain-gut axis Real-time PCR 16S rRNA Dysbiosis |
| url | https://doi.org/10.1186/s12866-023-02933-7 |
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