Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight
Early blight (EB) disease, caused mainly by Alternaria solani, is an economic threat to potato and tomato production worldwide. Thus, accurate and sensitive detection of the fungal pathogen of this disease in plants at the early infection stage is important for forecasting EB epidemics. In this stud...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016996/full |
| _version_ | 1798000487230865408 |
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| author | Zijian Niu Lijia Zheng Pan Yang Jinhui Wang Mengjun Tian Yang Pan Dongmei Zhao Zhihui Yang Jiehua Zhu |
| author_facet | Zijian Niu Lijia Zheng Pan Yang Jinhui Wang Mengjun Tian Yang Pan Dongmei Zhao Zhihui Yang Jiehua Zhu |
| author_sort | Zijian Niu |
| collection | DOAJ |
| description | Early blight (EB) disease, caused mainly by Alternaria solani, is an economic threat to potato and tomato production worldwide. Thus, accurate and sensitive detection of the fungal pathogen of this disease in plants at the early infection stage is important for forecasting EB epidemics. In this study, we developed an RNA-based method that enables highly accurate and sensitive A. solani detection in a whole potato leaf at a single spore level based on quantitative real-time polymerase chain reaction (qPCR). We discovered jg1677, a highly expressed gene whose full-length coding sequence is very specific for A. solani, by analyzing A. solani transcripts isolated from enhanced high throughput transcriptome of infected potato leaves by A. solani and using the National Center for Biotechnology Information’s basic local alignment search tool. The specificity of the primers derived from jg1677 was determined using 22 isolates of common potato pathogens, including seven Alternaria isolates. Detecting jg1677 transcripts with qPCR is 1,295 times more sensitive than detecting genomic DNA. In addition, the expression pattern of jg1677 at different infection stages was determined by qPCR. What is more, jg1677 was expressed relatively stable between 15 and 35°C in infected leaves, and its expression was virtually unaffected in isolated leaves left at room temperature for 24 h. Our work provides a much more sensitive and accurate method compared to conditional DNA-based ones, permitting a very early diagnosis of EB and lowering the risk of EB epidemics. |
| first_indexed | 2024-04-11T11:21:04Z |
| format | Article |
| id | doaj.art-e62cdc72053d45c59eb014f71bddfb93 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:21:04Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e62cdc72053d45c59eb014f71bddfb932022-12-22T04:27:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.10169961016996Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blightZijian Niu0Lijia Zheng1Pan Yang2Jinhui Wang3Mengjun Tian4Yang Pan5Dongmei Zhao6Zhihui Yang7Jiehua Zhu8College of Plant Protection, Hebei Agricultural University, Baoding, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaQinhuangdao Plant Protection and Quarantine Station, Qinhuangdao Agricultural and Rural Bureau, Qinhuangdao, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding, ChinaEarly blight (EB) disease, caused mainly by Alternaria solani, is an economic threat to potato and tomato production worldwide. Thus, accurate and sensitive detection of the fungal pathogen of this disease in plants at the early infection stage is important for forecasting EB epidemics. In this study, we developed an RNA-based method that enables highly accurate and sensitive A. solani detection in a whole potato leaf at a single spore level based on quantitative real-time polymerase chain reaction (qPCR). We discovered jg1677, a highly expressed gene whose full-length coding sequence is very specific for A. solani, by analyzing A. solani transcripts isolated from enhanced high throughput transcriptome of infected potato leaves by A. solani and using the National Center for Biotechnology Information’s basic local alignment search tool. The specificity of the primers derived from jg1677 was determined using 22 isolates of common potato pathogens, including seven Alternaria isolates. Detecting jg1677 transcripts with qPCR is 1,295 times more sensitive than detecting genomic DNA. In addition, the expression pattern of jg1677 at different infection stages was determined by qPCR. What is more, jg1677 was expressed relatively stable between 15 and 35°C in infected leaves, and its expression was virtually unaffected in isolated leaves left at room temperature for 24 h. Our work provides a much more sensitive and accurate method compared to conditional DNA-based ones, permitting a very early diagnosis of EB and lowering the risk of EB epidemics.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016996/fulldetection – plant pathogenspotatoearly blight diseaseAlternaria solaniRNA-based |
| spellingShingle | Zijian Niu Lijia Zheng Pan Yang Jinhui Wang Mengjun Tian Yang Pan Dongmei Zhao Zhihui Yang Jiehua Zhu Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight Frontiers in Microbiology detection – plant pathogens potato early blight disease Alternaria solani RNA-based |
| title | Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight |
| title_full | Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight |
| title_fullStr | Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight |
| title_full_unstemmed | Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight |
| title_short | Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight |
| title_sort | detection of alternaria solani with high accuracy and sensitivity during the latent period of potato early blight |
| topic | detection – plant pathogens potato early blight disease Alternaria solani RNA-based |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1016996/full |
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