Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018
In 2015, the Clinical and Laboratory Standards Institute (CLSI) updated its breakpoints for penicillin susceptibility in Corynebacterium species from <1 mg/L to <0.12 mg/L. We assessed the effect of this change on C. diphtheriae susceptibility reported at an inner city, tertiary care center in...
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2020-01-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/26/1/19-1241_article |
| _version_ | 1818841082173063168 |
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| author | Jason Zou Samuel D. Chorlton Marc G. Romney Michael Payne Tanya Lawson Anna Wong Sylvie Champagne Gordon Ritchie Christopher F. Lowe |
| author_facet | Jason Zou Samuel D. Chorlton Marc G. Romney Michael Payne Tanya Lawson Anna Wong Sylvie Champagne Gordon Ritchie Christopher F. Lowe |
| author_sort | Jason Zou |
| collection | DOAJ |
| description | In 2015, the Clinical and Laboratory Standards Institute (CLSI) updated its breakpoints for penicillin susceptibility in Corynebacterium species from <1 mg/L to <0.12 mg/L. We assessed the effect of this change on C. diphtheriae susceptibility reported at an inner city, tertiary care center in Vancouver, British Columbia, Canada, during 2015–2018 and performed whole-genome sequencing to investigate phenotypic and genotypic resistance to penicillin. We identified 44/45 isolates that were intermediately susceptible to penicillin by the 2015 breakpoint, despite meeting previous CLSI criteria for susceptibility. Sequencing did not reveal β-lactam resistance genes. Multilocus sequence typing revealed a notable predominance of sequence type 76. Overall, we saw no evidence of penicillin nonsusceptibility at the phenotypic or genotypic level in C. diphtheriae isolates from our institution. The 2015 CLSI breakpoint change could cause misclassification of penicillin susceptibility in C. diphtheriae isolates, potentially leading to suboptimal antimicrobial treatment selection. |
| first_indexed | 2024-12-19T04:20:25Z |
| format | Article |
| id | doaj.art-e62d219ea9934d11bebfe6c272064ad0 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-19T04:20:25Z |
| publishDate | 2020-01-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-e62d219ea9934d11bebfe6c272064ad02022-12-21T20:36:10ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592020-01-012619710310.3201/eid2601.191241Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018Jason ZouSamuel D. ChorltonMarc G. RomneyMichael PayneTanya LawsonAnna WongSylvie ChampagneGordon RitchieChristopher F. LoweIn 2015, the Clinical and Laboratory Standards Institute (CLSI) updated its breakpoints for penicillin susceptibility in Corynebacterium species from <1 mg/L to <0.12 mg/L. We assessed the effect of this change on C. diphtheriae susceptibility reported at an inner city, tertiary care center in Vancouver, British Columbia, Canada, during 2015–2018 and performed whole-genome sequencing to investigate phenotypic and genotypic resistance to penicillin. We identified 44/45 isolates that were intermediately susceptible to penicillin by the 2015 breakpoint, despite meeting previous CLSI criteria for susceptibility. Sequencing did not reveal β-lactam resistance genes. Multilocus sequence typing revealed a notable predominance of sequence type 76. Overall, we saw no evidence of penicillin nonsusceptibility at the phenotypic or genotypic level in C. diphtheriae isolates from our institution. The 2015 CLSI breakpoint change could cause misclassification of penicillin susceptibility in C. diphtheriae isolates, potentially leading to suboptimal antimicrobial treatment selection.https://wwwnc.cdc.gov/eid/article/26/1/19-1241_articleCorynebacterium diphtheriaediphtheriawhole genome sequencingpenicillinantimicrobial susceptibility, phenotypes, genotypes, bacteria, AMR, British Columbia, Canadawhole-genome sequencing |
| spellingShingle | Jason Zou Samuel D. Chorlton Marc G. Romney Michael Payne Tanya Lawson Anna Wong Sylvie Champagne Gordon Ritchie Christopher F. Lowe Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018 Emerging Infectious Diseases Corynebacterium diphtheriae diphtheria whole genome sequencing penicillin antimicrobial susceptibility, phenotypes, genotypes, bacteria, AMR, British Columbia, Canada whole-genome sequencing |
| title | Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018 |
| title_full | Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018 |
| title_fullStr | Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018 |
| title_full_unstemmed | Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018 |
| title_short | Phenotypic and Genotypic Correlates of Penicillin Susceptibility in Nontoxigenic Corynebacterium diphtheriae, British Columbia, Canada, 2015–2018 |
| title_sort | phenotypic and genotypic correlates of penicillin susceptibility in nontoxigenic corynebacterium diphtheriae british columbia canada 2015 2018 |
| topic | Corynebacterium diphtheriae diphtheria whole genome sequencing penicillin antimicrobial susceptibility, phenotypes, genotypes, bacteria, AMR, British Columbia, Canada whole-genome sequencing |
| url | https://wwwnc.cdc.gov/eid/article/26/1/19-1241_article |
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