Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets
Objectives: In non-small cell lung cancers (NSCLC), tumour biopsy can often be an invasive procedure. The development of a non-invasive methodology to study genetic changes via circulating tumour cells (CTCs) is an appealing concept. Whilst CTCs typically remain as rare cells, improvements in epitop...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6694/11/3/380 |
| _version_ | 1797723157522546688 |
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| author | Arutha Kulasinghe Joanna Kapeleris Carolina Cooper Majid Ebrahimi Warkiani Kenneth O’Byrne Chamindie Punyadeera |
| author_facet | Arutha Kulasinghe Joanna Kapeleris Carolina Cooper Majid Ebrahimi Warkiani Kenneth O’Byrne Chamindie Punyadeera |
| author_sort | Arutha Kulasinghe |
| collection | DOAJ |
| description | Objectives: In non-small cell lung cancers (NSCLC), tumour biopsy can often be an invasive procedure. The development of a non-invasive methodology to study genetic changes via circulating tumour cells (CTCs) is an appealing concept. Whilst CTCs typically remain as rare cells, improvements in epitope-independent CTC isolation techniques has given rise to a greater capture of CTCs. In this cross sectional study, we demonstrate the capture and characterization of NSCLC CTCs for the clinically actionable markers epidermal growth factor receptor (EGFR) alterations, anaplastic lymphoma kinase (ALK) rearrangements and programmed death ligand-1 (PD-L1) expression. The study identified CTCs/CTC clusters in 26/35 Stage IV NSCLC patients, and subsequently characterized the CTCs for EGFR mutation, ALK status and PD-L1 status. This pilot study demonstrates the potential of a non-invasive fluid biopsy to determine clinically relevant biomarkers in NSCLC. |
| first_indexed | 2024-03-12T09:57:53Z |
| format | Article |
| id | doaj.art-e62da5e2220e4b7098d34f50dcf11529 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T09:57:53Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e62da5e2220e4b7098d34f50dcf115292023-09-02T12:02:00ZengMDPI AGCancers2072-66942019-03-0111338010.3390/cancers11030380cancers11030380Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable TargetsArutha Kulasinghe0Joanna Kapeleris1Carolina Cooper2Majid Ebrahimi Warkiani3Kenneth O’Byrne4Chamindie Punyadeera5The School of Biomedical Sciences, Room 603D, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, AustraliaThe School of Biomedical Sciences, Room 603D, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, AustraliaDepartment of Anatomical Pathology, Pathology Queensland, QLD 4006, AustraliaThe School of Biomedical Engineering, University of Technology Sydney, Ultimo, NSW 2007, AustraliaTranslational Research Institute, Brisbane, QLD 4102, AustraliaThe School of Biomedical Sciences, Room 603D, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, AustraliaObjectives: In non-small cell lung cancers (NSCLC), tumour biopsy can often be an invasive procedure. The development of a non-invasive methodology to study genetic changes via circulating tumour cells (CTCs) is an appealing concept. Whilst CTCs typically remain as rare cells, improvements in epitope-independent CTC isolation techniques has given rise to a greater capture of CTCs. In this cross sectional study, we demonstrate the capture and characterization of NSCLC CTCs for the clinically actionable markers epidermal growth factor receptor (EGFR) alterations, anaplastic lymphoma kinase (ALK) rearrangements and programmed death ligand-1 (PD-L1) expression. The study identified CTCs/CTC clusters in 26/35 Stage IV NSCLC patients, and subsequently characterized the CTCs for EGFR mutation, ALK status and PD-L1 status. This pilot study demonstrates the potential of a non-invasive fluid biopsy to determine clinically relevant biomarkers in NSCLC.http://www.mdpi.com/2072-6694/11/3/380liquid biopsycirculating tumour cellsnon-small cell lung canceractionable mutations |
| spellingShingle | Arutha Kulasinghe Joanna Kapeleris Carolina Cooper Majid Ebrahimi Warkiani Kenneth O’Byrne Chamindie Punyadeera Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets Cancers liquid biopsy circulating tumour cells non-small cell lung cancer actionable mutations |
| title | Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets |
| title_full | Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets |
| title_fullStr | Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets |
| title_full_unstemmed | Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets |
| title_short | Phenotypic Characterization of Circulating Lung Cancer Cells for Clinically Actionable Targets |
| title_sort | phenotypic characterization of circulating lung cancer cells for clinically actionable targets |
| topic | liquid biopsy circulating tumour cells non-small cell lung cancer actionable mutations |
| url | http://www.mdpi.com/2072-6694/11/3/380 |
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