Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense
In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the eff...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-06-01
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| Series: | Folia Horticulturae |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/fhort-2022-0009 |
| _version_ | 1798042761713156096 |
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| author | Zhu Wangsheng Dai Jun Wang Jiahong |
| author_facet | Zhu Wangsheng Dai Jun Wang Jiahong |
| author_sort | Zhu Wangsheng |
| collection | DOAJ |
| description | In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the effects of Zn2+ concentrations on mitochondrial function and floral volatile emissions are yet to be revealed. Dendrobium huoshanense petals were used as materials and were treated with a 0–8 mM ZnSO4 solution. Intracellular Zn2+ concentrations were evaluated by the fluorescent dye method and atomic absorption method. Mitochondrial respiratory chain complex activities and the precursor and ATP contents were determined by the biochemical method. Floral volatile components were analyzed by the gas chromatograph-mass spectrometer (GC-MS). The results indicated that ZnSO4 application significantly increased intracellular Zn2+ concentrations. Elevated intracellular Zn2+ concentrations differently affected mitochondrial respiratory chain complex activities, precursor and adenosine triphosphate (ATP) productions and floral volatile emissions. Moreover, positive correlations exist among the activities of mitochondrial respiratory chain complexes, productions of precursors and ATP and emissions of floral volatiles. It is concluded that Zn2+ concentrations induce the activity changes of mitochondrial respiratory chain complexes, especially complex II and V, which promote or inhibit the emissions of floral volatiles by affecting the precursor and ATP levels that are closely related to the production of terpenoids, benzoids and fatty acid derivatives. The research will contribute to understanding the relationship between Zn2+ concentrations and floral volatile emissions from the perspective of mitochondrial function. |
| first_indexed | 2024-04-11T22:40:07Z |
| format | Article |
| id | doaj.art-e6301c7674084681bdc9c3b11269bc44 |
| institution | Directory Open Access Journal |
| issn | 2083-5965 |
| language | English |
| last_indexed | 2024-04-11T22:40:07Z |
| publishDate | 2022-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Horticulturae |
| spelling | doaj.art-e6301c7674084681bdc9c3b11269bc442022-12-22T03:59:03ZengSciendoFolia Horticulturae2083-59652022-06-0134110512410.2478/fhort-2022-0009Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanenseZhu Wangsheng0Dai Jun1Wang Jiahong2Engineering Technology Research Center for Plant Cell of Anhui Province, West Anhui University, Lu’an, ChinaEngineering Technology Research Center for Plant Cell of Anhui Province, West Anhui University, Lu’an, ChinaDepartment of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, ChinaIn recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the effects of Zn2+ concentrations on mitochondrial function and floral volatile emissions are yet to be revealed. Dendrobium huoshanense petals were used as materials and were treated with a 0–8 mM ZnSO4 solution. Intracellular Zn2+ concentrations were evaluated by the fluorescent dye method and atomic absorption method. Mitochondrial respiratory chain complex activities and the precursor and ATP contents were determined by the biochemical method. Floral volatile components were analyzed by the gas chromatograph-mass spectrometer (GC-MS). The results indicated that ZnSO4 application significantly increased intracellular Zn2+ concentrations. Elevated intracellular Zn2+ concentrations differently affected mitochondrial respiratory chain complex activities, precursor and adenosine triphosphate (ATP) productions and floral volatile emissions. Moreover, positive correlations exist among the activities of mitochondrial respiratory chain complexes, productions of precursors and ATP and emissions of floral volatiles. It is concluded that Zn2+ concentrations induce the activity changes of mitochondrial respiratory chain complexes, especially complex II and V, which promote or inhibit the emissions of floral volatiles by affecting the precursor and ATP levels that are closely related to the production of terpenoids, benzoids and fatty acid derivatives. The research will contribute to understanding the relationship between Zn2+ concentrations and floral volatile emissions from the perspective of mitochondrial function.https://doi.org/10.2478/fhort-2022-0009atpd. huoshanensefloral volatilesmitochondrial respiratory chain complexesprecursorszn2+ concentrations |
| spellingShingle | Zhu Wangsheng Dai Jun Wang Jiahong Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense Folia Horticulturae atp d. huoshanense floral volatiles mitochondrial respiratory chain complexes precursors zn2+ concentrations |
| title | Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense |
| title_full | Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense |
| title_fullStr | Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense |
| title_full_unstemmed | Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense |
| title_short | Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense |
| title_sort | zn2 induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in dendrobium huoshanense |
| topic | atp d. huoshanense floral volatiles mitochondrial respiratory chain complexes precursors zn2+ concentrations |
| url | https://doi.org/10.2478/fhort-2022-0009 |
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