Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells
Although emerging evidence links mesenchymal stem cells (MSCs) with cancer metastasis, the underlying mechanisms are poorly understood. In the present study, we found that human umbilical cord-derived MSCs (UC-MSCs) promoted MCF-7 cell migration in vitro and metastasis in vivo. To explore the mechan...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2015-12-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368915X687462 |
| _version_ | 1818257665365639168 |
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| author | Fengxia Ma Dandan Chen Fang Chen Ying Chi Zhibo Han Xiaoming Feng Xue Li Zhongchao Han |
| author_facet | Fengxia Ma Dandan Chen Fang Chen Ying Chi Zhibo Han Xiaoming Feng Xue Li Zhongchao Han |
| author_sort | Fengxia Ma |
| collection | DOAJ |
| description | Although emerging evidence links mesenchymal stem cells (MSCs) with cancer metastasis, the underlying mechanisms are poorly understood. In the present study, we found that human umbilical cord-derived MSCs (UC-MSCs) promoted MCF-7 cell migration in vitro and metastasis in vivo. To explore the mechanisms, the characteristics of MCF-7 cells cocultured with UC-MSCs were assessed. The expression and secretion of interleukin-8 (IL-8) and IL-6 were induced in MCF-7 cells cocultured with UC-MSCs. However, neutralization of IL-8 or IL-6 secreted by UC-MSCs could attenuate the enhanced expression of IL-8 and IL-6 in MCF-7 cells cocultured with UC-MSCs, which subsequently alleviated the enhanced migration. Similar to UC-MSCs, exogenous human recombinant IL-8 or IL-6 also promoted IL-8 and IL-6 expression and MCF-7 cell migration. In addition to enhanced IL-8 and IL-6 expression, MCF-7 cells cocultured with UC-MSCs displayed enhanced mammosphere-forming ability and increased percentage of CD44 + /CD24 - cells. However, epithelial-to-mesenchymal transition (EMT) was not observed in MCF-7 cells cocultured with UC-MSCs. Taken together, these results suggested that IL-8 and IL-6 secreted by UC-MSCs activated the autocrine IL-8 and IL-6 signaling in MCF-7 cells and induced CD44 + /CD24 - cells, which subsequently promoted MCF-7 cell migration in vitro and metastasis in vivo. |
| first_indexed | 2024-12-12T17:47:16Z |
| format | Article |
| id | doaj.art-e63029661de042c49b59af4c6e5e8509 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T17:47:16Z |
| publishDate | 2015-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-e63029661de042c49b59af4c6e5e85092022-12-22T00:16:53ZengSAGE PublishingCell Transplantation0963-68971555-38922015-12-012410.3727/096368915X687462Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 CellsFengxia Ma0Dandan Chen1Fang Chen2Ying Chi3Zhibo Han4Xiaoming Feng5Xue Li6Zhongchao Han7State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin, ChinaDepartment of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin, ChinaNational Engineering Research Center of Cell Products/AmCellGene Co. Ltd., Tianjin, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin, ChinaNational Engineering Research Center of Cell Products/AmCellGene Co. Ltd., Tianjin, ChinaAlthough emerging evidence links mesenchymal stem cells (MSCs) with cancer metastasis, the underlying mechanisms are poorly understood. In the present study, we found that human umbilical cord-derived MSCs (UC-MSCs) promoted MCF-7 cell migration in vitro and metastasis in vivo. To explore the mechanisms, the characteristics of MCF-7 cells cocultured with UC-MSCs were assessed. The expression and secretion of interleukin-8 (IL-8) and IL-6 were induced in MCF-7 cells cocultured with UC-MSCs. However, neutralization of IL-8 or IL-6 secreted by UC-MSCs could attenuate the enhanced expression of IL-8 and IL-6 in MCF-7 cells cocultured with UC-MSCs, which subsequently alleviated the enhanced migration. Similar to UC-MSCs, exogenous human recombinant IL-8 or IL-6 also promoted IL-8 and IL-6 expression and MCF-7 cell migration. In addition to enhanced IL-8 and IL-6 expression, MCF-7 cells cocultured with UC-MSCs displayed enhanced mammosphere-forming ability and increased percentage of CD44 + /CD24 - cells. However, epithelial-to-mesenchymal transition (EMT) was not observed in MCF-7 cells cocultured with UC-MSCs. Taken together, these results suggested that IL-8 and IL-6 secreted by UC-MSCs activated the autocrine IL-8 and IL-6 signaling in MCF-7 cells and induced CD44 + /CD24 - cells, which subsequently promoted MCF-7 cell migration in vitro and metastasis in vivo.https://doi.org/10.3727/096368915X687462 |
| spellingShingle | Fengxia Ma Dandan Chen Fang Chen Ying Chi Zhibo Han Xiaoming Feng Xue Li Zhongchao Han Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells Cell Transplantation |
| title | Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells |
| title_full | Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells |
| title_fullStr | Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells |
| title_full_unstemmed | Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells |
| title_short | Human Umbilical Cord Mesenchymal Stem Cells Promote Breast Cancer Metastasis by Interleukin-8- and Interleukin-6-Dependent Induction of CD44/CD24 Cells |
| title_sort | human umbilical cord mesenchymal stem cells promote breast cancer metastasis by interleukin 8 and interleukin 6 dependent induction of cd44 cd24 cells |
| url | https://doi.org/10.3727/096368915X687462 |
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