Influence of lipid rafts on pattern formation during T-cell adhesion
Adhesion of T cells to antigen presenting cells is mediated by the TCR–MHCp and LFA1–ICAM1 protein complexes. These intercellular protein complexes segregate and form characteristic special patterns in the cell contact zone. Previous studies have attempted to explain the mechanisms of formation of t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2021-01-01
|
| Series: | New Journal of Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1367-2630/abeacb |
| _version_ | 1797750176994033664 |
|---|---|
| author | Long Li Jinglei Hu Bartosz Różycki Xiaohuan Wang Helong Wu Fan Song |
| author_facet | Long Li Jinglei Hu Bartosz Różycki Xiaohuan Wang Helong Wu Fan Song |
| author_sort | Long Li |
| collection | DOAJ |
| description | Adhesion of T cells to antigen presenting cells is mediated by the TCR–MHCp and LFA1–ICAM1 protein complexes. These intercellular protein complexes segregate and form characteristic special patterns in the cell contact zone. Previous studies have attempted to explain the mechanisms of formation of these patterns. While emphasis has been put on membrane elasticity and active cytoskeletal transport, it remains unclear whether and how the pattern formation process is related to lipid rafts, which are nanoscale molecular clusters enriched in cholesterol and saturated phospholipids in cell membranes. Using Monte Carlo simulations of a statistical mechanical model for T-cell adhesion, we find that lipid rafts can lead to the formation of intermediate pattern with a ring of LFA1–ICAM1 complexes around a central domain of TCR–MHCp complexes even in the absence of active transport of T-cell receptor (TCR) molecules toward the center of the contact zone. In the presence of active TCR transport, lipid rafts can accelerate the formation of this monocentric pattern. We also find that lipid rafts have a strong stabilizing effect on the monocentric pattern after removal of the active TCR transport. Our results not only help to explain recent experimental observations, but also demonstrate that lipid rafts can cooperate with active cytoskeletal transport during the immunological synapse formation. |
| first_indexed | 2024-03-12T16:28:56Z |
| format | Article |
| id | doaj.art-e632ab7988824be0badeaf62cddab152 |
| institution | Directory Open Access Journal |
| issn | 1367-2630 |
| language | English |
| last_indexed | 2024-03-12T16:28:56Z |
| publishDate | 2021-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | New Journal of Physics |
| spelling | doaj.art-e632ab7988824be0badeaf62cddab1522023-08-08T15:36:37ZengIOP PublishingNew Journal of Physics1367-26302021-01-0123404305210.1088/1367-2630/abeacbInfluence of lipid rafts on pattern formation during T-cell adhesionLong Li0Jinglei Hu1https://orcid.org/0000-0002-5758-2879Bartosz Różycki2https://orcid.org/0000-0001-5938-7308Xiaohuan Wang3Helong Wu4https://orcid.org/0000-0002-7140-2184Fan Song5https://orcid.org/0000-0001-7359-2519State Key Laboratory of Nonlinear Mechanics and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences , Beijing 100190, People’s Republic of ChinaKuang Yaming Honors School & Institute for Brain Sciences, Nanjing University , Nanjing 210023, People’s Republic of China; Shenzhen Research Institute, Nanjing University , Shenzhen 518057, People's Republic of ChinaInstitute of Physics, Polish Academy of Sciences , Al. Lotników 32/46, Warsaw, PolandState Key Laboratory of Nonlinear Mechanics and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences , Beijing 100190, People’s Republic of China; School of Engineering Science, University of Chinese Academy of Sciences , Beijing 100049, People’s Republic of ChinaCollege of Mechanical Engineering, Zhejiang University of Technology , Hangzhou 310023, People’s Republic of ChinaState Key Laboratory of Nonlinear Mechanics and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences , Beijing 100190, People’s Republic of China; School of Engineering Science, University of Chinese Academy of Sciences , Beijing 100049, People’s Republic of ChinaAdhesion of T cells to antigen presenting cells is mediated by the TCR–MHCp and LFA1–ICAM1 protein complexes. These intercellular protein complexes segregate and form characteristic special patterns in the cell contact zone. Previous studies have attempted to explain the mechanisms of formation of these patterns. While emphasis has been put on membrane elasticity and active cytoskeletal transport, it remains unclear whether and how the pattern formation process is related to lipid rafts, which are nanoscale molecular clusters enriched in cholesterol and saturated phospholipids in cell membranes. Using Monte Carlo simulations of a statistical mechanical model for T-cell adhesion, we find that lipid rafts can lead to the formation of intermediate pattern with a ring of LFA1–ICAM1 complexes around a central domain of TCR–MHCp complexes even in the absence of active transport of T-cell receptor (TCR) molecules toward the center of the contact zone. In the presence of active TCR transport, lipid rafts can accelerate the formation of this monocentric pattern. We also find that lipid rafts have a strong stabilizing effect on the monocentric pattern after removal of the active TCR transport. Our results not only help to explain recent experimental observations, but also demonstrate that lipid rafts can cooperate with active cytoskeletal transport during the immunological synapse formation.https://doi.org/10.1088/1367-2630/abeacbT-cell adhesionimmunological synapsepattern formationlipid raftintercellular protein binding |
| spellingShingle | Long Li Jinglei Hu Bartosz Różycki Xiaohuan Wang Helong Wu Fan Song Influence of lipid rafts on pattern formation during T-cell adhesion New Journal of Physics T-cell adhesion immunological synapse pattern formation lipid raft intercellular protein binding |
| title | Influence of lipid rafts on pattern formation during T-cell adhesion |
| title_full | Influence of lipid rafts on pattern formation during T-cell adhesion |
| title_fullStr | Influence of lipid rafts on pattern formation during T-cell adhesion |
| title_full_unstemmed | Influence of lipid rafts on pattern formation during T-cell adhesion |
| title_short | Influence of lipid rafts on pattern formation during T-cell adhesion |
| title_sort | influence of lipid rafts on pattern formation during t cell adhesion |
| topic | T-cell adhesion immunological synapse pattern formation lipid raft intercellular protein binding |
| url | https://doi.org/10.1088/1367-2630/abeacb |
| work_keys_str_mv | AT longli influenceoflipidraftsonpatternformationduringtcelladhesion AT jingleihu influenceoflipidraftsonpatternformationduringtcelladhesion AT bartoszrozycki influenceoflipidraftsonpatternformationduringtcelladhesion AT xiaohuanwang influenceoflipidraftsonpatternformationduringtcelladhesion AT helongwu influenceoflipidraftsonpatternformationduringtcelladhesion AT fansong influenceoflipidraftsonpatternformationduringtcelladhesion |