Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis
Introduction: Liver fibrosis is the excessive accumulation of extracellular matrix that occurs by activation of hepatic stellate cells (HSCs), which has been identified as the major driver of liver fibrosis. Several studies confirmed that miRNAs have regulatory effects on the activation of HSCs by...
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| Format: | Article |
| Language: | English |
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Electronic Physician
2016-01-01
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| Series: | Electronic Physician |
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768932/ |
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| author | Eman El-Ahwany Faten Nagy Mona Zoheiry Mohamed Shemis Mona Nosseir Hoda Abu Taleb Maged El Ghannam Rafaat Atta Suher Zada Suher Zada |
| author_facet | Eman El-Ahwany Faten Nagy Mona Zoheiry Mohamed Shemis Mona Nosseir Hoda Abu Taleb Maged El Ghannam Rafaat Atta Suher Zada Suher Zada |
| author_sort | Eman El-Ahwany |
| collection | DOAJ |
| description | Introduction: Liver fibrosis is the excessive accumulation of extracellular matrix that occurs by activation of
hepatic stellate cells (HSCs), which has been identified as the major driver of liver fibrosis. Several studies
confirmed that miRNAs have regulatory effects on the activation of HSCs by affecting the signaling pathways.
The aim of this study was to develop non-invasive diagnostic markers by measuring different circulating
miRNAs in serum as predictor markers for early diagnosis of liver fibrosis and its progression.
Methods: In this case-control study, we enrolled 66 subjects with chronic hepatitis C (CHC) with early stage of
fibrosis and 65 subjects with CHC with late-stage fibrosis. Also, 40 subjects were included as normal controls.
The six main miRNAs, i.e., miR-138, miR-140, miR-143, miR-325, miR-328, and miR-349, were measured
using the reverse transcription-polymerase chain reaction.
Results: In the cases of CHC both with early and late stage of fibrosis, the circulating levels of the six main
miRNAs were significantly higher than the levels in the control group. ROC analysis indicated that the sensitivity
and specificity of miR-138 were 89.3% and 71.43%, respectively, in the early stage of fibrosis. In the late stage,
the sensitivity and specificity of miR-138 were 89.3 and 93.02%, respectively, whereas, for miR-143, they were
75.0 and 88.4%, respectively.
Conclusions: Circulating miR-138 could serve as a non-invasive biomarker for the detection of early fibrosis.
Also, miR-138 and miR-143 could be specific biomarkers for indicating the late stage of liver fibrosis |
| first_indexed | 2024-12-21T13:25:15Z |
| format | Article |
| id | doaj.art-e6338e3555bd46238fdd41e0c3cf4874 |
| institution | Directory Open Access Journal |
| issn | 2008-5842 2008-5842 |
| language | English |
| last_indexed | 2024-12-21T13:25:15Z |
| publishDate | 2016-01-01 |
| publisher | Electronic Physician |
| record_format | Article |
| series | Electronic Physician |
| spelling | doaj.art-e6338e3555bd46238fdd41e0c3cf48742022-12-21T19:02:28ZengElectronic PhysicianElectronic Physician2008-58422008-58422016-01-01811804181010.19082/1804Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver FibrosisEman El-AhwanyFaten NagyMona ZoheiryMohamed ShemisMona NosseirHoda Abu TalebMaged El GhannamRafaat AttaSuher ZadaSuher ZadaIntroduction: Liver fibrosis is the excessive accumulation of extracellular matrix that occurs by activation of hepatic stellate cells (HSCs), which has been identified as the major driver of liver fibrosis. Several studies confirmed that miRNAs have regulatory effects on the activation of HSCs by affecting the signaling pathways. The aim of this study was to develop non-invasive diagnostic markers by measuring different circulating miRNAs in serum as predictor markers for early diagnosis of liver fibrosis and its progression. Methods: In this case-control study, we enrolled 66 subjects with chronic hepatitis C (CHC) with early stage of fibrosis and 65 subjects with CHC with late-stage fibrosis. Also, 40 subjects were included as normal controls. The six main miRNAs, i.e., miR-138, miR-140, miR-143, miR-325, miR-328, and miR-349, were measured using the reverse transcription-polymerase chain reaction. Results: In the cases of CHC both with early and late stage of fibrosis, the circulating levels of the six main miRNAs were significantly higher than the levels in the control group. ROC analysis indicated that the sensitivity and specificity of miR-138 were 89.3% and 71.43%, respectively, in the early stage of fibrosis. In the late stage, the sensitivity and specificity of miR-138 were 89.3 and 93.02%, respectively, whereas, for miR-143, they were 75.0 and 88.4%, respectively. Conclusions: Circulating miR-138 could serve as a non-invasive biomarker for the detection of early fibrosis. Also, miR-138 and miR-143 could be specific biomarkers for indicating the late stage of liver fibrosishttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768932/liver fibrosismicroRNAschronic hepatitis C |
| spellingShingle | Eman El-Ahwany Faten Nagy Mona Zoheiry Mohamed Shemis Mona Nosseir Hoda Abu Taleb Maged El Ghannam Rafaat Atta Suher Zada Suher Zada Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis Electronic Physician liver fibrosis microRNAs chronic hepatitis C |
| title | Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis |
| title_full | Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis |
| title_fullStr | Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis |
| title_full_unstemmed | Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis |
| title_short | Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis |
| title_sort | circulating mirnas as predictor markers for activation of hepatic stellate cells and progression of hcv induced liver fibrosis |
| topic | liver fibrosis microRNAs chronic hepatitis C |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768932/ |
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