Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay
The unusual cases of pneumonia outbreak were reported from Wuhan city in late December 2019. Serological testing provides a powerful tool for the identification of prior infection and for epidemiological studies. Pseudotype virus neutralization assays are widely used for many viruses and application...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/6/994 |
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| author | Ahmed Majdi K. Tolah Sayed S. Sohrab Khaled Majdi K. Tolah Ahmed M. Hassan Sherif A. El-Kafrawy Esam I. Azhar |
| author_facet | Ahmed Majdi K. Tolah Sayed S. Sohrab Khaled Majdi K. Tolah Ahmed M. Hassan Sherif A. El-Kafrawy Esam I. Azhar |
| author_sort | Ahmed Majdi K. Tolah |
| collection | DOAJ |
| description | The unusual cases of pneumonia outbreak were reported from Wuhan city in late December 2019. Serological testing provides a powerful tool for the identification of prior infection and for epidemiological studies. Pseudotype virus neutralization assays are widely used for many viruses and applications in the fields of serology. The accuracy of pseudotype neutralizing assay allows for its use in low biosafety lab and provides a safe and effective alternative to the use of wild-type viruses. In this study, we evaluated the performance of this assay compared to the standard microneutralization assay as a reference. The lentiviral pseudotype particles were generated harboring the Spike gene of SARS-CoV-2. The generated pseudotype particles assay was used to evaluate the activity of neutralizing antibodies in 300 human serum samples from a COVID-19 sero-epidemiological study. Testing of these samples resulted in 55 positive samples and 245 negative samples by pseudotype viral particles assay while microneutralization assay resulted in 64 positive and 236 negative by MN assay. Compared to the MN, the pseudotyped viral particles assay showed a sensitivity of 85.94% and a specificity of 100%. Based on the data generated from this study, the pseudotype-based neutralization assay showed a reliable performance for the detection of neutralizing antibodies against SARS-CoV-2 and can be used safely and efficiently as a diagnostic tool in a biosafety level 2 laboratory. |
| first_indexed | 2024-03-10T10:53:09Z |
| format | Article |
| id | doaj.art-e63805dd1a624eb993d1e28acf16830c |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T10:53:09Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-e63805dd1a624eb993d1e28acf16830c2023-11-21T22:06:31ZengMDPI AGDiagnostics2075-44182021-05-0111699410.3390/diagnostics11060994Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization AssayAhmed Majdi K. Tolah0Sayed S. Sohrab1Khaled Majdi K. Tolah2Ahmed M. Hassan3Sherif A. El-Kafrawy4Esam I. Azhar5Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi ArabiaSpecial Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi ArabiaResident Preventive Medicine, Ministry of Health (MOH), P.O. Box 109385, Al Madinah Al Munnawarah 21351, Saudi ArabiaSpecial Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi ArabiaSpecial Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi ArabiaSpecial Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi ArabiaThe unusual cases of pneumonia outbreak were reported from Wuhan city in late December 2019. Serological testing provides a powerful tool for the identification of prior infection and for epidemiological studies. Pseudotype virus neutralization assays are widely used for many viruses and applications in the fields of serology. The accuracy of pseudotype neutralizing assay allows for its use in low biosafety lab and provides a safe and effective alternative to the use of wild-type viruses. In this study, we evaluated the performance of this assay compared to the standard microneutralization assay as a reference. The lentiviral pseudotype particles were generated harboring the Spike gene of SARS-CoV-2. The generated pseudotype particles assay was used to evaluate the activity of neutralizing antibodies in 300 human serum samples from a COVID-19 sero-epidemiological study. Testing of these samples resulted in 55 positive samples and 245 negative samples by pseudotype viral particles assay while microneutralization assay resulted in 64 positive and 236 negative by MN assay. Compared to the MN, the pseudotyped viral particles assay showed a sensitivity of 85.94% and a specificity of 100%. Based on the data generated from this study, the pseudotype-based neutralization assay showed a reliable performance for the detection of neutralizing antibodies against SARS-CoV-2 and can be used safely and efficiently as a diagnostic tool in a biosafety level 2 laboratory.https://www.mdpi.com/2075-4418/11/6/994SARS-CoV-2pseudotypecoronavirusmicro-neutralization assaySaudi Arabia |
| spellingShingle | Ahmed Majdi K. Tolah Sayed S. Sohrab Khaled Majdi K. Tolah Ahmed M. Hassan Sherif A. El-Kafrawy Esam I. Azhar Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay Diagnostics SARS-CoV-2 pseudotype coronavirus micro-neutralization assay Saudi Arabia |
| title | Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay |
| title_full | Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay |
| title_fullStr | Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay |
| title_full_unstemmed | Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay |
| title_short | Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay |
| title_sort | evaluation of a pseudovirus neutralization assay for sars cov 2 and correlation with live virus based micro neutralization assay |
| topic | SARS-CoV-2 pseudotype coronavirus micro-neutralization assay Saudi Arabia |
| url | https://www.mdpi.com/2075-4418/11/6/994 |
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