High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels
Picobirnaviruses (PBVs) are small non-enveloped bisegmented double-stranded RNA viruses found in humans, mammals, and birds. Increasing molecular epidemiology studies suggest a high sequence diversity of PBVs in numerous hosts and the environment. In this study, using 229 fecal samples from dromedar...
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| Formato: | Artículo |
| Lenguaje: | English |
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MDPI AG
2021-03-01
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| Colección: | Viruses |
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| Acceso en línea: | https://www.mdpi.com/1999-4915/13/3/430 |
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| author | Jade L. L. Teng Ulrich Wernery Po Chun Wong Elaine Chan Hwei Huih Lee Sunitha Joseph Ru Bai Ying Tang Emily Y. M. Wong Susanna K. P. Lau Patrick C. Y. Woo |
| author_facet | Jade L. L. Teng Ulrich Wernery Po Chun Wong Elaine Chan Hwei Huih Lee Sunitha Joseph Ru Bai Ying Tang Emily Y. M. Wong Susanna K. P. Lau Patrick C. Y. Woo |
| author_sort | Jade L. L. Teng |
| collection | DOAJ |
| description | Picobirnaviruses (PBVs) are small non-enveloped bisegmented double-stranded RNA viruses found in humans, mammals, and birds. Increasing molecular epidemiology studies suggest a high sequence diversity of PBVs in numerous hosts and the environment. In this study, using 229 fecal samples from dromedary camels in Dubai, 52.8% were positive for PBVs, of which 77.7% and 41.3% were positive for genogroup I and II, respectively, and 19.0% were positive for both genotypes. Phylogenetic analysis showed high diversity among the sequences of genogroup I and II dromedary PBVs. Marked nucleotide polymorphisms were observed in 75.5% and 46.0% of genogroup I and II RNA-dependent RNA polymerase (RdRp) sequences, respectively, suggesting the co-existence of multiple strains in the same specimen. Both high genetic diversity and prevalence of genogroup I and II PBV in dromedaries were observed. In fact, the prevalence of genogroup II PBV in dromedaries is the highest among all animals to date. The complete/near-complete core genomes of five genogroup I and one genogroup II dromedary PBVs and partial segment 1 and 2 of both genotypes were also sequenced. The dromedary PBV genome organizations were similar to those of other animals. Genetic reassortment and mutation are both important in the ecology and evolution of PBVs. |
| first_indexed | 2024-03-09T05:00:35Z |
| format | Article |
| id | doaj.art-e63a12cf90ca44d49db819e8a74e8c51 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:00:35Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e63a12cf90ca44d49db819e8a74e8c512023-12-03T13:00:55ZengMDPI AGViruses1999-49152021-03-0113343010.3390/v13030430High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary CamelsJade L. L. Teng0Ulrich Wernery1Po Chun Wong2Elaine Chan3Hwei Huih Lee4Sunitha Joseph5Ru Bai6Ying Tang7Emily Y. M. Wong8Susanna K. P. Lau9Patrick C. Y. Woo10Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaCentral Veterinary Research Laboratory, P.O. Box 597, Dubai, United Arab EmiratesDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaCentral Veterinary Research Laboratory, P.O. Box 597, Dubai, United Arab EmiratesDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, ChinaPicobirnaviruses (PBVs) are small non-enveloped bisegmented double-stranded RNA viruses found in humans, mammals, and birds. Increasing molecular epidemiology studies suggest a high sequence diversity of PBVs in numerous hosts and the environment. In this study, using 229 fecal samples from dromedary camels in Dubai, 52.8% were positive for PBVs, of which 77.7% and 41.3% were positive for genogroup I and II, respectively, and 19.0% were positive for both genotypes. Phylogenetic analysis showed high diversity among the sequences of genogroup I and II dromedary PBVs. Marked nucleotide polymorphisms were observed in 75.5% and 46.0% of genogroup I and II RNA-dependent RNA polymerase (RdRp) sequences, respectively, suggesting the co-existence of multiple strains in the same specimen. Both high genetic diversity and prevalence of genogroup I and II PBV in dromedaries were observed. In fact, the prevalence of genogroup II PBV in dromedaries is the highest among all animals to date. The complete/near-complete core genomes of five genogroup I and one genogroup II dromedary PBVs and partial segment 1 and 2 of both genotypes were also sequenced. The dromedary PBV genome organizations were similar to those of other animals. Genetic reassortment and mutation are both important in the ecology and evolution of PBVs.https://www.mdpi.com/1999-4915/13/3/430picobirnavirusesdromedarygenogroup Igenogroup IIdiversity |
| spellingShingle | Jade L. L. Teng Ulrich Wernery Po Chun Wong Elaine Chan Hwei Huih Lee Sunitha Joseph Ru Bai Ying Tang Emily Y. M. Wong Susanna K. P. Lau Patrick C. Y. Woo High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels Viruses picobirnaviruses dromedary genogroup I genogroup II diversity |
| title | High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels |
| title_full | High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels |
| title_fullStr | High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels |
| title_full_unstemmed | High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels |
| title_short | High Prevalence of Genogroup I and Genogroup II Picobirnaviruses in Dromedary Camels |
| title_sort | high prevalence of genogroup i and genogroup ii picobirnaviruses in dromedary camels |
| topic | picobirnaviruses dromedary genogroup I genogroup II diversity |
| url | https://www.mdpi.com/1999-4915/13/3/430 |
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