In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs
Chronic otitis externa of dogs is a significant problem due to the prevalence and complexity of the treatment of such animals. There is evidence that in 60–80% of cases of infectious diseases microorganisms located in the biofilm phenotype play the main role. Microorganisms in the biofilm phenotype...
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MDPI AG
2023-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/7/1259 |
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| author | Ifarajimi Rapheal Olabode Nadezhda Sachivkina Arfenia Karamyan Ramziya Mannapova Olga Kuznetsova Anna Bobunova Natallia Zhabo Marina Avdonina Regina Gurina |
| author_facet | Ifarajimi Rapheal Olabode Nadezhda Sachivkina Arfenia Karamyan Ramziya Mannapova Olga Kuznetsova Anna Bobunova Natallia Zhabo Marina Avdonina Regina Gurina |
| author_sort | Ifarajimi Rapheal Olabode |
| collection | DOAJ |
| description | Chronic otitis externa of dogs is a significant problem due to the prevalence and complexity of the treatment of such animals. There is evidence that in 60–80% of cases of infectious diseases microorganisms located in the biofilm phenotype play the main role. Microorganisms in the biofilm phenotype have a number of advantages, the most significant of which is considered to be increased resistance to various external factors. Among them, a special place is occupied by resistance to antibiotics. In recent decades, research has been conducted at an increasing scale on the role of biofilm infections in various pathologies in veterinary medicine. The etiology and therapy of dog otitis externa caused by <i>Malassezia pachydermatis</i> biofilm has not been fully studied. This is why we consider relevant the scientific and practical aspects of research on the etiology and therapy of dog otitis externa from the position of biofilm infection. In this work, it has been statistically proven that there is a relationship between the optical density of <i>Malassezia pachydermatis</i> biofilms and their sensitivity to drugs, and this relationship is statistically significant. In addition, we have demonstrated that Farnesol has a good antibiofilm effect at a concentration of more 1.6 μM/mL (24% OD decrease of biofilm), and its highest antibiofilm effect (71–55%—more than a half) was observed at a concentration of 200–12.5 μM/mL. |
| first_indexed | 2024-03-11T05:44:17Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T05:44:17Z |
| publishDate | 2023-04-01 |
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| series | Animals |
| spelling | doaj.art-e63ca3af63c649f488776f9ed752f7f72023-11-17T16:14:57ZengMDPI AGAnimals2076-26152023-04-01137125910.3390/ani13071259In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in DogsIfarajimi Rapheal Olabode0Nadezhda Sachivkina1Arfenia Karamyan2Ramziya Mannapova3Olga Kuznetsova4Anna Bobunova5Natallia Zhabo6Marina Avdonina7Regina Gurina8Department of Veterinary Medicine, Agrarian Technological Institute, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Agrarian Technological Institute, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, 127434 Moscow, RussiaDepartment of Biochemistry T.T. Berezov, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Foreign Languages, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Foreign Languages, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Linguistics and Intercultural Communication, Moscow State Linguistic University, 119034 Moscow, RussiaDepartment of Technosphere Safety, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaChronic otitis externa of dogs is a significant problem due to the prevalence and complexity of the treatment of such animals. There is evidence that in 60–80% of cases of infectious diseases microorganisms located in the biofilm phenotype play the main role. Microorganisms in the biofilm phenotype have a number of advantages, the most significant of which is considered to be increased resistance to various external factors. Among them, a special place is occupied by resistance to antibiotics. In recent decades, research has been conducted at an increasing scale on the role of biofilm infections in various pathologies in veterinary medicine. The etiology and therapy of dog otitis externa caused by <i>Malassezia pachydermatis</i> biofilm has not been fully studied. This is why we consider relevant the scientific and practical aspects of research on the etiology and therapy of dog otitis externa from the position of biofilm infection. In this work, it has been statistically proven that there is a relationship between the optical density of <i>Malassezia pachydermatis</i> biofilms and their sensitivity to drugs, and this relationship is statistically significant. In addition, we have demonstrated that Farnesol has a good antibiofilm effect at a concentration of more 1.6 μM/mL (24% OD decrease of biofilm), and its highest antibiofilm effect (71–55%—more than a half) was observed at a concentration of 200–12.5 μM/mL.https://www.mdpi.com/2076-2615/13/7/1259Farnesoldogs<i>Malassezia pachydermatis</i>antifungal susceptibilityquorum-sensing molecules |
| spellingShingle | Ifarajimi Rapheal Olabode Nadezhda Sachivkina Arfenia Karamyan Ramziya Mannapova Olga Kuznetsova Anna Bobunova Natallia Zhabo Marina Avdonina Regina Gurina In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs Animals Farnesol dogs <i>Malassezia pachydermatis</i> antifungal susceptibility quorum-sensing molecules |
| title | In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs |
| title_full | In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs |
| title_fullStr | In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs |
| title_full_unstemmed | In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs |
| title_short | In Vitro Activity of Farnesol against <i>Malassezia pachydermatis</i> Isolates from Otitis Externa Cases in Dogs |
| title_sort | in vitro activity of farnesol against i malassezia pachydermatis i isolates from otitis externa cases in dogs |
| topic | Farnesol dogs <i>Malassezia pachydermatis</i> antifungal susceptibility quorum-sensing molecules |
| url | https://www.mdpi.com/2076-2615/13/7/1259 |
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