Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.)
Commercial papaya varieties grown in Australia vary greatly in taste and aroma. Previous profiling has identified undesirable ‘off tastes’ in existing varieties, discouraging a portion of the population from consuming papayas. Our focus on enhancing preferred flavours led to an exploration of the ge...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/3046 |
| _version_ | 1797264402505793536 |
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| author | Ziwei Zhou Chutchamas Kanchana-udomkan Rebecca Ford Ido Bar |
| author_facet | Ziwei Zhou Chutchamas Kanchana-udomkan Rebecca Ford Ido Bar |
| author_sort | Ziwei Zhou |
| collection | DOAJ |
| description | Commercial papaya varieties grown in Australia vary greatly in taste and aroma. Previous profiling has identified undesirable ‘off tastes’ in existing varieties, discouraging a portion of the population from consuming papayas. Our focus on enhancing preferred flavours led to an exploration of the genetic mechanisms and biosynthesis pathways that underlie these desired taste profiles. To identify genes associated with consumer-preferred flavours, we conducted whole RNA sequencing and de novo genome assembly on papaya varieties RB1 (known for its sweet flavour and floral aroma) and 1B (less favoured due to its bitter taste and musty aroma) at both ripe and unripe stages. In total, 180,368 transcripts were generated, and 118 transcripts related to flavours were differentially expressed between the two varieties at the ripe stage. Five genes (<i>cpBGH3B</i>, <i>cpPFP</i>, <i>cpSUS</i>, <i>cpGES</i> and <i>cpLIS</i>) were validated through qPCR and significantly differentially expressed. These genes are suggested to play key roles in sucrose metabolism and aromatic compound production pathways, holding promise for future selective breeding strategies. Further exploration will involve assessing their potential across broader germplasm and various growth environments. |
| first_indexed | 2024-04-25T00:28:20Z |
| format | Article |
| id | doaj.art-e63cd838132941b78307077f8e038ced |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:28:20Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e63cd838132941b78307077f8e038ced2024-03-12T16:47:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255304610.3390/ijms25053046Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.)Ziwei Zhou0Chutchamas Kanchana-udomkan1Rebecca Ford2Ido Bar3Centre for Planetary Health and Food Security, School of Environment and Science, Griffith University, Nathan, QLD 4111, AustraliaCentre for Planetary Health and Food Security, School of Environment and Science, Griffith University, Nathan, QLD 4111, AustraliaCentre for Planetary Health and Food Security, School of Environment and Science, Griffith University, Nathan, QLD 4111, AustraliaCentre for Planetary Health and Food Security, School of Environment and Science, Griffith University, Nathan, QLD 4111, AustraliaCommercial papaya varieties grown in Australia vary greatly in taste and aroma. Previous profiling has identified undesirable ‘off tastes’ in existing varieties, discouraging a portion of the population from consuming papayas. Our focus on enhancing preferred flavours led to an exploration of the genetic mechanisms and biosynthesis pathways that underlie these desired taste profiles. To identify genes associated with consumer-preferred flavours, we conducted whole RNA sequencing and de novo genome assembly on papaya varieties RB1 (known for its sweet flavour and floral aroma) and 1B (less favoured due to its bitter taste and musty aroma) at both ripe and unripe stages. In total, 180,368 transcripts were generated, and 118 transcripts related to flavours were differentially expressed between the two varieties at the ripe stage. Five genes (<i>cpBGH3B</i>, <i>cpPFP</i>, <i>cpSUS</i>, <i>cpGES</i> and <i>cpLIS</i>) were validated through qPCR and significantly differentially expressed. These genes are suggested to play key roles in sucrose metabolism and aromatic compound production pathways, holding promise for future selective breeding strategies. Further exploration will involve assessing their potential across broader germplasm and various growth environments.https://www.mdpi.com/1422-0067/25/5/3046RNA sequencingsweetness genesRT-qPCRdifferential expressionde novo genome assembly |
| spellingShingle | Ziwei Zhou Chutchamas Kanchana-udomkan Rebecca Ford Ido Bar Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.) International Journal of Molecular Sciences RNA sequencing sweetness genes RT-qPCR differential expression de novo genome assembly |
| title | Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.) |
| title_full | Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.) |
| title_fullStr | Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.) |
| title_full_unstemmed | Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.) |
| title_short | Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (<i>Carica papaya</i> L.) |
| title_sort | identification and validation of key genes related to preferred flavour profiles in australian commercial papaya i carica papaya i l |
| topic | RNA sequencing sweetness genes RT-qPCR differential expression de novo genome assembly |
| url | https://www.mdpi.com/1422-0067/25/5/3046 |
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