Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera
Nutrition is vital for health and immune function in honey bees (Apis mellifera). The effect of diets enriched with bee-associated yeasts and essential oils of Mexican oregano (Lippia graveolens) was tested on survival, food intake, accumulated fat body tissue, and gene expression of vitellogenin (V...
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| Format: | Article |
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PeerJ Inc.
2021-10-01
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| Online Access: | https://peerj.com/articles/12164.pdf |
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| author | César Canché-Collí Humberto Estrella-Maldonado Luis A. Medina-Medina Humberto Moo-Valle Luz Maria Calvo-Irabien Elisa Chan-Vivas Rosalina Rodríguez Azucena Canto |
| author_facet | César Canché-Collí Humberto Estrella-Maldonado Luis A. Medina-Medina Humberto Moo-Valle Luz Maria Calvo-Irabien Elisa Chan-Vivas Rosalina Rodríguez Azucena Canto |
| author_sort | César Canché-Collí |
| collection | DOAJ |
| description | Nutrition is vital for health and immune function in honey bees (Apis mellifera). The effect of diets enriched with bee-associated yeasts and essential oils of Mexican oregano (Lippia graveolens) was tested on survival, food intake, accumulated fat body tissue, and gene expression of vitellogenin (Vg), prophenoloxidase (proPO) and glucose oxidase (GOx) in newly emerged worker bees. The enriched diets were provided to bees under the premise that supplementation with yeasts or essential oils can enhance health variables and the expression of genes related to immune function in worker bees. Based on a standard pollen substitute, used as a control diet, enriched diets were formulated, five with added bee-associated yeasts (Starmerella bombicola, Starmerella etchellsii, Starmerella bombicola 2, Zygosaccharomyces mellis, and the brewers’ yeast Saccharomyces cerevisiae) and three with added essential oils from L. graveolens (carvacrol, thymol, and sesquiterpenes). Groups of bees were fed one of the diets for 9 or 12 days. Survival probability was similar in the yeast and essential oils treatments in relation to the control, but median survival was lower in the carvacrol and sesquiterpenes treatments. Food intake was higher in all the yeast treatments than in the control. Fat body percentage in individual bees was slightly lower in all treatments than in the control, with significant decreases in the thymol and carvacrol treatments. Expression of the genes Vg, proPO, and GOx was minimally affected by the yeast treatments but was adversely affected by the carvacrol and thymol treatments. |
| first_indexed | 2024-03-09T07:54:04Z |
| format | Article |
| id | doaj.art-e63fae18500249ed85f4d2f73b6409d4 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:54:04Z |
| publishDate | 2021-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-e63fae18500249ed85f4d2f73b6409d42023-12-03T01:21:58ZengPeerJ Inc.PeerJ2167-83592021-10-019e1216410.7717/peerj.12164Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis melliferaCésar Canché-Collí0Humberto Estrella-Maldonado1Luis A. Medina-Medina2Humberto Moo-Valle3Luz Maria Calvo-Irabien4Elisa Chan-Vivas5Rosalina Rodríguez6Azucena Canto7Unidad de Recursos Naturales, Centro de Investigacion Cientifica de Yucatan, A.C., Merida, Yucatan, MexicoUnidad de Recursos Naturales, Centro de Investigacion Cientifica de Yucatan, A.C., Merida, Yucatan, MexicoDepartamento de Apicultura, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan, Merida, Yucatan, MexicoDepartamento de Apicultura, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan, Merida, Yucatan, MexicoUnidad de Recursos Naturales, Centro de Investigacion Cientifica de Yucatan, A.C., Merida, Yucatan, MexicoUnidad de Recursos Naturales, Centro de Investigacion Cientifica de Yucatan, A.C., Merida, Yucatan, MexicoUnidad de Recursos Naturales, Centro de Investigacion Cientifica de Yucatan, A.C., Merida, Yucatan, MexicoUnidad de Recursos Naturales, Centro de Investigacion Cientifica de Yucatan, A.C., Merida, Yucatan, MexicoNutrition is vital for health and immune function in honey bees (Apis mellifera). The effect of diets enriched with bee-associated yeasts and essential oils of Mexican oregano (Lippia graveolens) was tested on survival, food intake, accumulated fat body tissue, and gene expression of vitellogenin (Vg), prophenoloxidase (proPO) and glucose oxidase (GOx) in newly emerged worker bees. The enriched diets were provided to bees under the premise that supplementation with yeasts or essential oils can enhance health variables and the expression of genes related to immune function in worker bees. Based on a standard pollen substitute, used as a control diet, enriched diets were formulated, five with added bee-associated yeasts (Starmerella bombicola, Starmerella etchellsii, Starmerella bombicola 2, Zygosaccharomyces mellis, and the brewers’ yeast Saccharomyces cerevisiae) and three with added essential oils from L. graveolens (carvacrol, thymol, and sesquiterpenes). Groups of bees were fed one of the diets for 9 or 12 days. Survival probability was similar in the yeast and essential oils treatments in relation to the control, but median survival was lower in the carvacrol and sesquiterpenes treatments. Food intake was higher in all the yeast treatments than in the control. Fat body percentage in individual bees was slightly lower in all treatments than in the control, with significant decreases in the thymol and carvacrol treatments. Expression of the genes Vg, proPO, and GOx was minimally affected by the yeast treatments but was adversely affected by the carvacrol and thymol treatments.https://peerj.com/articles/12164.pdfHoneybeeNutritionImmune systemBee-associated yeastsEssential oilPollen substitutes |
| spellingShingle | César Canché-Collí Humberto Estrella-Maldonado Luis A. Medina-Medina Humberto Moo-Valle Luz Maria Calvo-Irabien Elisa Chan-Vivas Rosalina Rodríguez Azucena Canto Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera PeerJ Honeybee Nutrition Immune system Bee-associated yeasts Essential oil Pollen substitutes |
| title | Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera |
| title_full | Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera |
| title_fullStr | Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera |
| title_full_unstemmed | Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera |
| title_short | Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera |
| title_sort | effect of yeast and essential oil enriched diets on critical determinants of health and immune function in africanized apis mellifera |
| topic | Honeybee Nutrition Immune system Bee-associated yeasts Essential oil Pollen substitutes |
| url | https://peerj.com/articles/12164.pdf |
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