Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i>
The diamondback moth, <i>Plutella xylostella</i> (L.) is an important pest of cruciferous crops worldwide. It has developed resistance to many conventional and novel insecticide classes. Metaflumizone belongs to the new chemical class of semicarbazone insecticides. To delay the developme...
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MDPI AG
2020-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/5/311 |
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| author | Jun Shen Zhao Li Dongyang Li Rumeng Wang Shuzhen Zhang Hong You Jianhong Li |
| author_facet | Jun Shen Zhao Li Dongyang Li Rumeng Wang Shuzhen Zhang Hong You Jianhong Li |
| author_sort | Jun Shen |
| collection | DOAJ |
| description | The diamondback moth, <i>Plutella xylostella</i> (L.) is an important pest of cruciferous crops worldwide. It has developed resistance to many conventional and novel insecticide classes. Metaflumizone belongs to the new chemical class of semicarbazone insecticides. To delay the development of metaflumizone resistance in <i>P. xylostella</i> and to guide insecticide use in the field, the biochemical mechanisms, cross-resistance spectrum, and stability of resistance to metaflumizone were studied in a laboratory-selected resistant strain (metaflu-SEL). Synergism tests with the carboxylesterase inhibitor triphenyl phosphate (TPP), the glutathione S-transferase depletor diethyl maleate (DEM), and the P450 inhibitor piperonyl butoxide(PBO) had no obvious effect on metaflumizone in the metaflu-SEL strain and the susceptible strain (SS) of <i>P. xylostella</i>, with synergism ratios that ranged from 1.02 to 1.86. Biochemical studies revealed that the cytochrome P450-dependent monooxygenase increased only 1.13-fold in the metaflu-SEL strain compared with the UNSEL stain; meanwhile, carboxylesterase and glutathione <i>S</i>-transferase activity showed no difference. These results suggest that these detoxification enzymes may be not actively involved in metaflumizone resistance. Furthermore, the metaflu-SEL population showed a moderate level of cross-resistance to indoxacarb (11.63-fold), but only very low cross-resistance to spinosad (1.75-fold), spinetoram (3.52-fold), abamectin (2.81-fold), beta-cypermethrin (0.71-fold), diafenthiuron (0.79-fold), chlorantraniliprole (2.16-fold), BT (WG-001) (3.34-fold), chlorfenapyr (0.49-fold), and chlorfluazuron (0.97-fold). Moreover, metaflumizone resistance decreased from 1087.85- to 1.23-fold in the metaflu-SEL strain after 12 generations without exposure to metaflumizone. These results are useful for formulating insecticide resistance management strategies to control <i>P. xylostella</i> and to delay the development of metaflumizone resistance in the field. |
| first_indexed | 2024-03-10T19:50:04Z |
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| institution | Directory Open Access Journal |
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| language | English |
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| spelling | doaj.art-e640daabb7914b5e947856ab5d8657582023-11-20T00:32:13ZengMDPI AGInsects2075-44502020-05-0111531110.3390/insects11050311Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i>Jun Shen0Zhao Li1Dongyang Li2Rumeng Wang3Shuzhen Zhang4Hong You5Jianhong Li6College of Horticulture, Xinyang Agriculture and Forestry University, Xinyang 464000, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaThe diamondback moth, <i>Plutella xylostella</i> (L.) is an important pest of cruciferous crops worldwide. It has developed resistance to many conventional and novel insecticide classes. Metaflumizone belongs to the new chemical class of semicarbazone insecticides. To delay the development of metaflumizone resistance in <i>P. xylostella</i> and to guide insecticide use in the field, the biochemical mechanisms, cross-resistance spectrum, and stability of resistance to metaflumizone were studied in a laboratory-selected resistant strain (metaflu-SEL). Synergism tests with the carboxylesterase inhibitor triphenyl phosphate (TPP), the glutathione S-transferase depletor diethyl maleate (DEM), and the P450 inhibitor piperonyl butoxide(PBO) had no obvious effect on metaflumizone in the metaflu-SEL strain and the susceptible strain (SS) of <i>P. xylostella</i>, with synergism ratios that ranged from 1.02 to 1.86. Biochemical studies revealed that the cytochrome P450-dependent monooxygenase increased only 1.13-fold in the metaflu-SEL strain compared with the UNSEL stain; meanwhile, carboxylesterase and glutathione <i>S</i>-transferase activity showed no difference. These results suggest that these detoxification enzymes may be not actively involved in metaflumizone resistance. Furthermore, the metaflu-SEL population showed a moderate level of cross-resistance to indoxacarb (11.63-fold), but only very low cross-resistance to spinosad (1.75-fold), spinetoram (3.52-fold), abamectin (2.81-fold), beta-cypermethrin (0.71-fold), diafenthiuron (0.79-fold), chlorantraniliprole (2.16-fold), BT (WG-001) (3.34-fold), chlorfenapyr (0.49-fold), and chlorfluazuron (0.97-fold). Moreover, metaflumizone resistance decreased from 1087.85- to 1.23-fold in the metaflu-SEL strain after 12 generations without exposure to metaflumizone. These results are useful for formulating insecticide resistance management strategies to control <i>P. xylostella</i> and to delay the development of metaflumizone resistance in the field.https://www.mdpi.com/2075-4450/11/5/311detoxification enzymescross-resistancemetaflumizone<i>Plutella xylostella</i>resistance |
| spellingShingle | Jun Shen Zhao Li Dongyang Li Rumeng Wang Shuzhen Zhang Hong You Jianhong Li Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i> Insects detoxification enzymes cross-resistance metaflumizone <i>Plutella xylostella</i> resistance |
| title | Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i> |
| title_full | Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i> |
| title_fullStr | Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i> |
| title_full_unstemmed | Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i> |
| title_short | Biochemical Mechanisms, Cross-resistance and Stability of Resistance to Metaflumizone in <i>Plutella xylostella</i> |
| title_sort | biochemical mechanisms cross resistance and stability of resistance to metaflumizone in i plutella xylostella i |
| topic | detoxification enzymes cross-resistance metaflumizone <i>Plutella xylostella</i> resistance |
| url | https://www.mdpi.com/2075-4450/11/5/311 |
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