Magnetic bead technology for viral RNA extraction from serum in blood bank screening
Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. Objective: Manual mag...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-11-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867011702495 |
| _version_ | 1819104344283283456 |
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| author | Guilherme Ambrozio Albertoni, PhD Students Carine Prisco Arnoni, PhD Students Patricia Regina Barboza Araujo, PhD Students Sheila Siqueira Andrade, PhD Students Fabrício Oliveira Carvalho Manoel João Batista Castello Girão Nestor Schor José Augusto Barreto |
| author_facet | Guilherme Ambrozio Albertoni, PhD Students Carine Prisco Arnoni, PhD Students Patricia Regina Barboza Araujo, PhD Students Sheila Siqueira Andrade, PhD Students Fabrício Oliveira Carvalho Manoel João Batista Castello Girão Nestor Schor José Augusto Barreto |
| author_sort | Guilherme Ambrozio Albertoni, PhD Students |
| collection | DOAJ |
| description | Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. Objective: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase -polymerase chain reaction (RT-PCR) one-step. Methods: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. Results: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. Conclusion: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. Keywords: HCV, HIV, infection, reverse transcriptase polymerase chain reaction, blood banks |
| first_indexed | 2024-12-22T02:04:51Z |
| format | Article |
| id | doaj.art-e641f555bac447c7b830f28834c94bbd |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-22T02:04:51Z |
| publishDate | 2011-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-e641f555bac447c7b830f28834c94bbd2022-12-21T18:42:34ZengElsevierBrazilian Journal of Infectious Diseases1413-86702011-11-01156547552Magnetic bead technology for viral RNA extraction from serum in blood bank screeningGuilherme Ambrozio Albertoni, PhD Students0Carine Prisco Arnoni, PhD Students1Patricia Regina Barboza Araujo, PhD Students2Sheila Siqueira Andrade, PhD Students3Fabrício Oliveira Carvalho4Manoel João Batista Castello Girão5Nestor Schor6José Augusto Barreto7Colsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, Brazil; Correspondence to: Av. Jandira, 1260 Indianópolis 04080-006 São Paulo, SP Brazil Phone: +55 11 5055-6588 R-39 Fax: +55 11 5055-6588.Colsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, BrazilColsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, BrazilColsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, BrazilColsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, BrazilColsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, BrazilUniversidade Federal de São Paulo (UNIFESP), SP, BrazilHead, Colsan – Associação Beneficente de Coleta de Sangue, São Paulo, SP, BrazilNucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. Objective: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase -polymerase chain reaction (RT-PCR) one-step. Methods: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. Results: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. Conclusion: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. Keywords: HCV, HIV, infection, reverse transcriptase polymerase chain reaction, blood bankshttp://www.sciencedirect.com/science/article/pii/S1413867011702495 |
| spellingShingle | Guilherme Ambrozio Albertoni, PhD Students Carine Prisco Arnoni, PhD Students Patricia Regina Barboza Araujo, PhD Students Sheila Siqueira Andrade, PhD Students Fabrício Oliveira Carvalho Manoel João Batista Castello Girão Nestor Schor José Augusto Barreto Magnetic bead technology for viral RNA extraction from serum in blood bank screening Brazilian Journal of Infectious Diseases |
| title | Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
| title_full | Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
| title_fullStr | Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
| title_full_unstemmed | Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
| title_short | Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
| title_sort | magnetic bead technology for viral rna extraction from serum in blood bank screening |
| url | http://www.sciencedirect.com/science/article/pii/S1413867011702495 |
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