Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System

Background: Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present s...

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Main Authors: Irina B Zakharova, Yana A Lopasteyskaya, Andrey V Toporkov, Dmitry V Viktorov
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2018-01-01
Series:Journal of Global Infectious Diseases
Subjects:
Online Access:http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=1;spage=7;epage=10;aulast=Zakharova
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author Irina B Zakharova
Yana A Lopasteyskaya
Andrey V Toporkov
Dmitry V Viktorov
author_facet Irina B Zakharova
Yana A Lopasteyskaya
Andrey V Toporkov
Dmitry V Viktorov
author_sort Irina B Zakharova
collection DOAJ
description Background: Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present study was to identify the biochemical features of B. pseudomallei, which can affect its correct identification by Vitek 2 system. Materials and Methods: The biochemical patterns of 40 B. pseudomallei strains were obtained using Vitek 2 GN cards. The average contribution of biochemical tests in overall dissimilarities between correctly and incorrectly identified strains was assessed using nonmetric multidimensional scaling. Results: It was found (R statistic of 0.836, P = 0.001) that a combination of negative N-acetyl galactosaminidase, β-N-acetyl glucosaminidase, phosphatase, and positive D-cellobiase (dCEL), tyrosine arylamidase (TyrA), and L-proline arylamidase (ProA) tests leads to low discrimination of B. pseudomallei, whereas a set of positive dCEL and negative N-acetyl galactosaminidase, TyrA, and ProA determines the wrong identification of B. pseudomallei as Burkholderia cepacia complex. Conclusion: The further expansion of the Vitek 2 identification keys is needed for correct identification of atypical or regionally distributed biochemical profiles of B. pseudomallei.
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spelling doaj.art-e6432fe614914d539b3c598102bbcf882022-12-22T03:35:40ZengWolters Kluwer Medknow PublicationsJournal of Global Infectious Diseases0974-777X2018-01-0110171010.4103/jgid.jgid_39_17Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 SystemIrina B ZakharovaYana A LopasteyskayaAndrey V ToporkovDmitry V ViktorovBackground: Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present study was to identify the biochemical features of B. pseudomallei, which can affect its correct identification by Vitek 2 system. Materials and Methods: The biochemical patterns of 40 B. pseudomallei strains were obtained using Vitek 2 GN cards. The average contribution of biochemical tests in overall dissimilarities between correctly and incorrectly identified strains was assessed using nonmetric multidimensional scaling. Results: It was found (R statistic of 0.836, P = 0.001) that a combination of negative N-acetyl galactosaminidase, β-N-acetyl glucosaminidase, phosphatase, and positive D-cellobiase (dCEL), tyrosine arylamidase (TyrA), and L-proline arylamidase (ProA) tests leads to low discrimination of B. pseudomallei, whereas a set of positive dCEL and negative N-acetyl galactosaminidase, TyrA, and ProA determines the wrong identification of B. pseudomallei as Burkholderia cepacia complex. Conclusion: The further expansion of the Vitek 2 identification keys is needed for correct identification of atypical or regionally distributed biochemical profiles of B. pseudomallei.http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=1;spage=7;epage=10;aulast=ZakharovaBiochemical identificationBurkholderia cepaciaBurkholderia pseudomalleinonmetric multidimensional scalingVitek 2
spellingShingle Irina B Zakharova
Yana A Lopasteyskaya
Andrey V Toporkov
Dmitry V Viktorov
Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
Journal of Global Infectious Diseases
Biochemical identification
Burkholderia cepacia
Burkholderia pseudomallei
nonmetric multidimensional scaling
Vitek 2
title Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
title_full Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
title_fullStr Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
title_full_unstemmed Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
title_short Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
title_sort influence of biochemical features of burkholderia pseudomallei strains on identification reliability by vitek 2 system
topic Biochemical identification
Burkholderia cepacia
Burkholderia pseudomallei
nonmetric multidimensional scaling
Vitek 2
url http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=1;spage=7;epage=10;aulast=Zakharova
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