Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System
Background: Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present s...
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Format: | Article |
Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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Series: | Journal of Global Infectious Diseases |
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Online Access: | http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=1;spage=7;epage=10;aulast=Zakharova |
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author | Irina B Zakharova Yana A Lopasteyskaya Andrey V Toporkov Dmitry V Viktorov |
author_facet | Irina B Zakharova Yana A Lopasteyskaya Andrey V Toporkov Dmitry V Viktorov |
author_sort | Irina B Zakharova |
collection | DOAJ |
description | Background: Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present study was to identify the biochemical features of B. pseudomallei, which can affect its correct identification by Vitek 2 system. Materials and Methods: The biochemical patterns of 40 B. pseudomallei strains were obtained using Vitek 2 GN cards. The average contribution of biochemical tests in overall dissimilarities between correctly and incorrectly identified strains was assessed using nonmetric multidimensional scaling. Results: It was found (R statistic of 0.836, P = 0.001) that a combination of negative N-acetyl galactosaminidase, β-N-acetyl glucosaminidase, phosphatase, and positive D-cellobiase (dCEL), tyrosine arylamidase (TyrA), and L-proline arylamidase (ProA) tests leads to low discrimination of B. pseudomallei, whereas a set of positive dCEL and negative N-acetyl galactosaminidase, TyrA, and ProA determines the wrong identification of B. pseudomallei as Burkholderia cepacia complex. Conclusion: The further expansion of the Vitek 2 identification keys is needed for correct identification of atypical or regionally distributed biochemical profiles of B. pseudomallei. |
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format | Article |
id | doaj.art-e6432fe614914d539b3c598102bbcf88 |
institution | Directory Open Access Journal |
issn | 0974-777X |
language | English |
last_indexed | 2024-04-12T11:08:29Z |
publishDate | 2018-01-01 |
publisher | Wolters Kluwer Medknow Publications |
record_format | Article |
series | Journal of Global Infectious Diseases |
spelling | doaj.art-e6432fe614914d539b3c598102bbcf882022-12-22T03:35:40ZengWolters Kluwer Medknow PublicationsJournal of Global Infectious Diseases0974-777X2018-01-0110171010.4103/jgid.jgid_39_17Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 SystemIrina B ZakharovaYana A LopasteyskayaAndrey V ToporkovDmitry V ViktorovBackground: Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present study was to identify the biochemical features of B. pseudomallei, which can affect its correct identification by Vitek 2 system. Materials and Methods: The biochemical patterns of 40 B. pseudomallei strains were obtained using Vitek 2 GN cards. The average contribution of biochemical tests in overall dissimilarities between correctly and incorrectly identified strains was assessed using nonmetric multidimensional scaling. Results: It was found (R statistic of 0.836, P = 0.001) that a combination of negative N-acetyl galactosaminidase, β-N-acetyl glucosaminidase, phosphatase, and positive D-cellobiase (dCEL), tyrosine arylamidase (TyrA), and L-proline arylamidase (ProA) tests leads to low discrimination of B. pseudomallei, whereas a set of positive dCEL and negative N-acetyl galactosaminidase, TyrA, and ProA determines the wrong identification of B. pseudomallei as Burkholderia cepacia complex. Conclusion: The further expansion of the Vitek 2 identification keys is needed for correct identification of atypical or regionally distributed biochemical profiles of B. pseudomallei.http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=1;spage=7;epage=10;aulast=ZakharovaBiochemical identificationBurkholderia cepaciaBurkholderia pseudomalleinonmetric multidimensional scalingVitek 2 |
spellingShingle | Irina B Zakharova Yana A Lopasteyskaya Andrey V Toporkov Dmitry V Viktorov Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System Journal of Global Infectious Diseases Biochemical identification Burkholderia cepacia Burkholderia pseudomallei nonmetric multidimensional scaling Vitek 2 |
title | Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System |
title_full | Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System |
title_fullStr | Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System |
title_full_unstemmed | Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System |
title_short | Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2 System |
title_sort | influence of biochemical features of burkholderia pseudomallei strains on identification reliability by vitek 2 system |
topic | Biochemical identification Burkholderia cepacia Burkholderia pseudomallei nonmetric multidimensional scaling Vitek 2 |
url | http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=1;spage=7;epage=10;aulast=Zakharova |
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