Phage-Based Control of Methicillin Resistant <i>Staphylococcus aureus</i> in a <i>Galleria mellonella</i> Model of Implant-Associated Infection
<i>Staphylococcus aureus</i> implant-associated infections are difficult to treat because of the ability of bacteria to form biofilm on medical devices. Here, the efficacy of Sb-1 to control or prevent <i>S. aureus</i> colonization on medical foreign bodies was investigated i...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/23/14514 |
| Summary: | <i>Staphylococcus aureus</i> implant-associated infections are difficult to treat because of the ability of bacteria to form biofilm on medical devices. Here, the efficacy of Sb-1 to control or prevent <i>S. aureus</i> colonization on medical foreign bodies was investigated in a <i>Galleria mellonella</i> larval infection model. For colonization control assays, sterile K-wires were implanted into larva prolegs. After 2 days, larvae were infected with methicillin-resistant <i>S. aureus</i> ATCC 43300 and incubated at 37 °C for a further 2 days, when treatments with either daptomycin (4 mg/kg), Sb-1 (10<sup>7</sup> PFUs) or a combination of them (3 x/day) were started. For biofilm prevention assays, larvae were pre-treated with either vancomycin (10 mg/kg) or Sb-1 (10<sup>7</sup> PFUs) before the <i>S. aureus</i> infection. In both experimental settings, K-wires were explanted for colony counting two days after treatment. In comparison to the untreated control, more than a 4 log<sup>10</sup> CFU and 1 log<sup>10</sup> CFU reduction was observed on K-wires recovered from larvae treated with the Sb-1/daptomycin combination and with their singular administration, respectively. Moreover, pre-infection treatment with Sb-1 was found to prevent K-wire colonization, similarly to vancomycin. Taken together, the obtained results demonstrated the strong potential of the Sb-1 antibiotic combinatory administration or the Sb-1 pretreatment to control or prevent <i>S. aureus</i>-associated implant infections. |
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| ISSN: | 1661-6596 1422-0067 |