Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes
Emodin is an anthraquinone derivative from rhubarb and buckthorn, and is also produced by many species of fungi. Although its anti-diabetic effect in type 2 diabetes has been reported, the potential effects of emodin on type 1 diabetes remain unclear. Therefore, in this study, we isolated emodin fro...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615001899 |
| _version_ | 1819294330355974144 |
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| author | Ui-Jin Bae Mi-Young Song Hyun-Young Jang Jung Min Lim So Yoon Lee Jae-Ha Ryu Byung-Hyun Park |
| author_facet | Ui-Jin Bae Mi-Young Song Hyun-Young Jang Jung Min Lim So Yoon Lee Jae-Ha Ryu Byung-Hyun Park |
| author_sort | Ui-Jin Bae |
| collection | DOAJ |
| description | Emodin is an anthraquinone derivative from rhubarb and buckthorn, and is also produced by many species of fungi. Although its anti-diabetic effect in type 2 diabetes has been reported, the potential effects of emodin on type 1 diabetes remain unclear. Therefore, in this study, we isolated emodin from rhubarb (Rheum palmatum) and investigated its effects on cytokine- or streptozotocin-induced β-cell damage and dysfunction. Emodin protected RINm5F cells or mouse islets against cytokine-induced damage by suppressing inducible nitric oxide synthase expression and nitric oxide production. The cytoprotective potential of emodin was associated with the inhibition of NF-κB signaling by reducing IκB kinase activity. The protective effects of emodin were further demonstrated by normalizing insulin secretion of cytokine-treated islets in response to glucose. In multiple low-dose streptozotocin-induced diabetes model, emodin-pretreated mice were fully resistant to the development of diabetes with normal islet morphology and insulin secretion. Immunohistochemical analysis revealed that emodin significantly decreased the number of TUNEL-positive and nuclear p65-positive pancreatic islets. These results suggest that emodin blocks the NF-κB pathway, thus protecting β-cells against diabetogenic insults. Emodin may have therapeutic value in preventing the development of type 1 diabetes by delaying the process of insulitis and by protecting against β-cell damage. |
| first_indexed | 2024-12-24T04:24:36Z |
| format | Article |
| id | doaj.art-e644c3b8f79745f4add304ad4915dcfe |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:24:36Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e644c3b8f79745f4add304ad4915dcfe2022-12-21T17:15:41ZengElsevierJournal of Functional Foods1756-46462015-06-0116919Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetesUi-Jin Bae0Mi-Young Song1Hyun-Young Jang2Jung Min Lim3So Yoon Lee4Jae-Ha Ryu5Byung-Hyun Park6Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of KoreaDepartment of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of KoreaDepartment of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of KoreaDepartment of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of KoreaResearch Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of KoreaResearch Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea; Corresponding author. College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Ku, Seoul 140-742, Republic of Korea. Tel.: +82 2 710 9568; fax: +82 2 714 0745.Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of Korea; Corresponding author. Chonbuk National University Medical School, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Republic of Korea. Tel.: +82 63 270 3139; fax: +82 63 274 9833.Emodin is an anthraquinone derivative from rhubarb and buckthorn, and is also produced by many species of fungi. Although its anti-diabetic effect in type 2 diabetes has been reported, the potential effects of emodin on type 1 diabetes remain unclear. Therefore, in this study, we isolated emodin from rhubarb (Rheum palmatum) and investigated its effects on cytokine- or streptozotocin-induced β-cell damage and dysfunction. Emodin protected RINm5F cells or mouse islets against cytokine-induced damage by suppressing inducible nitric oxide synthase expression and nitric oxide production. The cytoprotective potential of emodin was associated with the inhibition of NF-κB signaling by reducing IκB kinase activity. The protective effects of emodin were further demonstrated by normalizing insulin secretion of cytokine-treated islets in response to glucose. In multiple low-dose streptozotocin-induced diabetes model, emodin-pretreated mice were fully resistant to the development of diabetes with normal islet morphology and insulin secretion. Immunohistochemical analysis revealed that emodin significantly decreased the number of TUNEL-positive and nuclear p65-positive pancreatic islets. These results suggest that emodin blocks the NF-κB pathway, thus protecting β-cells against diabetogenic insults. Emodin may have therapeutic value in preventing the development of type 1 diabetes by delaying the process of insulitis and by protecting against β-cell damage.http://www.sciencedirect.com/science/article/pii/S1756464615001899EmodinNF-κBCytokineStreptozotocinDiabetesApoptosis |
| spellingShingle | Ui-Jin Bae Mi-Young Song Hyun-Young Jang Jung Min Lim So Yoon Lee Jae-Ha Ryu Byung-Hyun Park Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes Journal of Functional Foods Emodin NF-κB Cytokine Streptozotocin Diabetes Apoptosis |
| title | Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes |
| title_full | Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes |
| title_fullStr | Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes |
| title_full_unstemmed | Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes |
| title_short | Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes |
| title_sort | emodin isolated from rheum palmatum prevents cytokine induced β cell damage and the development of type 1 diabetes |
| topic | Emodin NF-κB Cytokine Streptozotocin Diabetes Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615001899 |
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