Comparative Colonization of hilA and Parent Strains of Salmonella enteritidis in Fertile Eggs
<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> ...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2016-08-01
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Series: | Journal of Medical Bacteriology |
Subjects: | |
Online Access: | http://jmb.tums.ac.ir/index.php/jmb/article/view/186 |
Summary: | <div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> Young chickens are more susceptible to <em>Salmonella</em> colonization than older ones that have developed resistance with age as native microflora become established. </p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> In this study, two groups of fertile eggs were inoculated with 20 CFU of <em>hilA</em> or parent strains of <em>S</em>. <em>enteritidis</em>. Presence and number of <em>Salmonella</em> cells inside the homogenized egg contents were determined on the 2nd, 5th, 8th, 12th, 17th and 21th day of incubation period. </p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> High infectivity rate of <em>Salmonella</em> contamination were observed in the <em>hilA</em> group eggs, three genes for <em>S. enteritidis</em> identification were detected from isolated colonies of both groups of eggs. The gene <em>hilA</em> was only detected in isolated colonies of the standard group. </p><p><strong><em>Conclusion</em></strong><strong>:<em> </em></strong> These findings indicated that <em>hilA</em> mutant of <em>Salmonella</em> is able to rapidly multiply much higher than wild-type strain but, support more pathogenicity of wild-type strain of <em>Salmonella</em> compared to mutant strain.</p></td></tr></tbody></table></div> |
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ISSN: | 2251-8649 2322-2581 |