Comparative Colonization of hilA and Parent Strains of Salmonella enteritidis in Fertile Eggs

<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> ...

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Bibliographic Details
Main Authors: Mohammad Sadegh Madadi, Mohammad Hassanzadeh, Fateme Karimnezhad
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2016-08-01
Series:Journal of Medical Bacteriology
Subjects:
Online Access:http://jmb.tums.ac.ir/index.php/jmb/article/view/186
Description
Summary:<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> Young chickens are more susceptible to <em>Salmonella</em> colonization than older ones that have developed resistance with age as native microflora become established. </p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> In this study, two groups of fertile eggs were inoculated with 20 CFU of <em>hilA</em> or parent strains of <em>S</em>. <em>enteritidis</em>. Presence and number of <em>Salmonella</em> cells inside the homogenized egg contents were determined on the 2nd, 5th, 8th, 12th, 17th and 21th day of incubation period. </p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> High infectivity rate of <em>Salmonella</em> contamination were observed in the <em>hilA</em> group eggs, three genes for <em>S. enteritidis</em> identification were detected from isolated colonies of both groups of eggs.  The gene <em>hilA</em> was only detected in isolated colonies of the standard group. </p><p><strong><em>Conclusion</em></strong><strong>:<em> </em></strong>  These findings indicated that <em>hilA</em> mutant of <em>Salmonella</em> is able to rapidly multiply much higher than wild-type strain but, support more pathogenicity of wild-type strain of <em>Salmonella</em> compared to mutant strain.</p></td></tr></tbody></table></div>
ISSN:2251-8649
2322-2581