Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial
Abstract Background Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 positively affect the fecal bacteriome in children with celiac disease autoimmunity after 6 months of supplementation. The aim of the present investigation was to study the effects of Lactiplantibacillus...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-023-06027-1 |
| _version_ | 1797630413725761536 |
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| author | Jakub Hurych Elin Oscarsson Åsa Håkanson Kateřina Jirků-Pomajbíková Milan Jirků Carin Andrén Aronson Ondřej Cinek Daniel Agardh the HEDIMED Investigator Group |
| author_facet | Jakub Hurych Elin Oscarsson Åsa Håkanson Kateřina Jirků-Pomajbíková Milan Jirků Carin Andrén Aronson Ondřej Cinek Daniel Agardh the HEDIMED Investigator Group |
| author_sort | Jakub Hurych |
| collection | DOAJ |
| description | Abstract Background Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 positively affect the fecal bacteriome in children with celiac disease autoimmunity after 6 months of supplementation. The aim of the present investigation was to study the effects of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 on the single-cell parasitome, with a primary focus on Blastocystis. Methods Stool samples were collected from 78 Swedish children with celiac disease autoimmunity participating in a randomized, double-blind, placebo-controlled clinical trial to either receive a mixture of supplementation with L. plantarum HEAL9 and L. paracasei 8700:2 (n = 38) or placebo (n = 40). A total of 227 stool samples collected at baseline and after 3 and 6 months of intervention, respectively, were retrospectively analyzed for Blastocystis by quantitative real-time PCR and subtyped by massively parallel amplicon sequencing. Other single-cell parasites were detected by untargeted 18S rDNA amplicon sequencing and verified by real-time PCR. The relation between the parasites and the bacteriome community was characterized by using 16S rDNA profiling of the V3-V4 region. Results Three different single-cell protists were identified, of which the highest prevalence was found for Dientamoeba fragilis (23.1%, 18/78 children), followed by Blastocystis (15.4%, 12/78) and Entamoeba spp. (2.6%, 2/78). The quantity of the protists was stable over time and not affected by probiotic intervention (P = 0.14 for Blastocystis, P = 0.10 for D. fragilis). The positivity of the protists was associated with increased bacteriome diversity (measured by multiple indices, P < 0.03). Bacterial composition was influenced by the presence of the protists: positivity of Blastocystis was inversely associated with Akkermansia (at the levels of the genus as well as its family, order, class and phylum); P < 0.002), Faecalibacterium (P = 0.003) and Romboutsia (P = 0.029); positivity of D. fragilis was inversely associated with families Enterobacteriaceae (P = 0.016) and Coriobacteriaceae (P = 0.022) and genera Flavonifractor (P < 0.001), Faecalibacterium (P = 0.009), Lachnoclostridium (P = 0.029), Ruminococcus (P < 0.001) and Granulicatella (P = 0.018). Conclusions The prevalence of single-cell protists is low in children with celiac disease autoimmunity. The colonization was stable regardless of the probiotic intervention and associated with increased diversity of the fecal bacteriome but inversely associated with some beneficial bacteria. Graphical Abstract |
| first_indexed | 2024-03-11T11:07:56Z |
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| id | doaj.art-e64c0850809f4f59a1e517f3d9d2cbf0 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-11T11:07:56Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-e64c0850809f4f59a1e517f3d9d2cbf02023-11-12T12:08:10ZengBMCParasites & Vectors1756-33052023-11-0116111310.1186/s13071-023-06027-1Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trialJakub Hurych0Elin Oscarsson1Åsa Håkanson2Kateřina Jirků-Pomajbíková3Milan Jirků4Carin Andrén Aronson5Ondřej Cinek6Daniel Agardh7the HEDIMED Investigator GroupDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles UniversityDepartment of Food Technology, Engineering and Nutrition, Lund UniversityDepartment of Food Technology, Engineering and Nutrition, Lund UniversityInstitute of Parasitology, Biology Centre, Czech Academy of SciencesInstitute of Parasitology, Biology Centre, Czech Academy of SciencesCeliac Disease and Diabetes Unit, Department of Clinical Sciences, Lund UniversityDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles UniversityCeliac Disease and Diabetes Unit, Department of Clinical Sciences, Lund UniversityAbstract Background Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 positively affect the fecal bacteriome in children with celiac disease autoimmunity after 6 months of supplementation. The aim of the present investigation was to study the effects of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 on the single-cell parasitome, with a primary focus on Blastocystis. Methods Stool samples were collected from 78 Swedish children with celiac disease autoimmunity participating in a randomized, double-blind, placebo-controlled clinical trial to either receive a mixture of supplementation with L. plantarum HEAL9 and L. paracasei 8700:2 (n = 38) or placebo (n = 40). A total of 227 stool samples collected at baseline and after 3 and 6 months of intervention, respectively, were retrospectively analyzed for Blastocystis by quantitative real-time PCR and subtyped by massively parallel amplicon sequencing. Other single-cell parasites were detected by untargeted 18S rDNA amplicon sequencing and verified by real-time PCR. The relation between the parasites and the bacteriome community was characterized by using 16S rDNA profiling of the V3-V4 region. Results Three different single-cell protists were identified, of which the highest prevalence was found for Dientamoeba fragilis (23.1%, 18/78 children), followed by Blastocystis (15.4%, 12/78) and Entamoeba spp. (2.6%, 2/78). The quantity of the protists was stable over time and not affected by probiotic intervention (P = 0.14 for Blastocystis, P = 0.10 for D. fragilis). The positivity of the protists was associated with increased bacteriome diversity (measured by multiple indices, P < 0.03). Bacterial composition was influenced by the presence of the protists: positivity of Blastocystis was inversely associated with Akkermansia (at the levels of the genus as well as its family, order, class and phylum); P < 0.002), Faecalibacterium (P = 0.003) and Romboutsia (P = 0.029); positivity of D. fragilis was inversely associated with families Enterobacteriaceae (P = 0.016) and Coriobacteriaceae (P = 0.022) and genera Flavonifractor (P < 0.001), Faecalibacterium (P = 0.009), Lachnoclostridium (P = 0.029), Ruminococcus (P < 0.001) and Granulicatella (P = 0.018). Conclusions The prevalence of single-cell protists is low in children with celiac disease autoimmunity. The colonization was stable regardless of the probiotic intervention and associated with increased diversity of the fecal bacteriome but inversely associated with some beneficial bacteria. Graphical Abstracthttps://doi.org/10.1186/s13071-023-06027-1BlastocystisDientamoeba fragilisCeliac diseaseProbioticsGut microbiome |
| spellingShingle | Jakub Hurych Elin Oscarsson Åsa Håkanson Kateřina Jirků-Pomajbíková Milan Jirků Carin Andrén Aronson Ondřej Cinek Daniel Agardh the HEDIMED Investigator Group Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial Parasites & Vectors Blastocystis Dientamoeba fragilis Celiac disease Probiotics Gut microbiome |
| title | Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial |
| title_full | Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial |
| title_fullStr | Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial |
| title_full_unstemmed | Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial |
| title_short | Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial |
| title_sort | effects of lactiplantibacillus plantarum and lacticaseibacillus paracasei supplementation on the single cell fecal parasitome in children with celiac disease autoimmunity a randomized double blind placebo controlled clinical trial |
| topic | Blastocystis Dientamoeba fragilis Celiac disease Probiotics Gut microbiome |
| url | https://doi.org/10.1186/s13071-023-06027-1 |
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