Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID)
<abstract language="eng">Methyl ethyl ketone (MEK) is a solvent commonly used in chemical, paint and shoe industry. The aim of this study was to develop and validate a method for urinary quantification of MEK, employing headspace solid phase micro extraction sampling (SPME) coupled t...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2008-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800043 |
| _version_ | 1819213319643332608 |
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| author | Marina Venzon Antunes Rafael Linden |
| author_facet | Marina Venzon Antunes Rafael Linden |
| author_sort | Marina Venzon Antunes |
| collection | DOAJ |
| description | <abstract language="eng">Methyl ethyl ketone (MEK) is a solvent commonly used in chemical, paint and shoe industry. The aim of this study was to develop and validate a method for urinary quantification of MEK, employing headspace solid phase micro extraction sampling (SPME) coupled to gas chromatography with flame ionization detection (GC-FID). The calibration curve (y=4.6851x-0.0011) presented good linearity with r²=0.9993. Accuracy (94-109%), intra-assay precision (4.07-5.91%) and inter-assay precision (3.03-5.62%) were acceptable. The quantification limit was 0.19 mg/L. This low cost method can be used routinely in the biological monitoring of occupational exposure to MEK, according to the requirements of the Brazilian legislation. |
| first_indexed | 2024-12-23T06:56:58Z |
| format | Article |
| id | doaj.art-e64c09d297f743a9b2031dfca361a378 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-23T06:56:58Z |
| publishDate | 2008-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-e64c09d297f743a9b2031dfca361a3782022-12-21T17:56:18ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642008-01-013182165216810.1590/S0100-40422008000800043Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID)Marina Venzon AntunesRafael Linden<abstract language="eng">Methyl ethyl ketone (MEK) is a solvent commonly used in chemical, paint and shoe industry. The aim of this study was to develop and validate a method for urinary quantification of MEK, employing headspace solid phase micro extraction sampling (SPME) coupled to gas chromatography with flame ionization detection (GC-FID). The calibration curve (y=4.6851x-0.0011) presented good linearity with r²=0.9993. Accuracy (94-109%), intra-assay precision (4.07-5.91%) and inter-assay precision (3.03-5.62%) were acceptable. The quantification limit was 0.19 mg/L. This low cost method can be used routinely in the biological monitoring of occupational exposure to MEK, according to the requirements of the Brazilian legislation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800043methyl ethyl ketoneSPME-GC-FIDbiological monitoring |
| spellingShingle | Marina Venzon Antunes Rafael Linden Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID) Química Nova methyl ethyl ketone SPME-GC-FID biological monitoring |
| title | Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID) |
| title_full | Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID) |
| title_fullStr | Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID) |
| title_full_unstemmed | Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID) |
| title_short | Determinação de metil-etil-cetona em amostras de urina com amostragem por micro extração em fase sólida (MEFS) em headspace associada à cromatografia gasosa com detector de ionização de chama (CG-DIC) Determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction (SPME) sampling associated to gas chromatography with flame-ionization detectoR (GC-FID) |
| title_sort | determinacao de metil etil cetona em amostras de urina com amostragem por micro extracao em fase solida mefs em headspace associada a cromatografia gasosa com detector de ionizacao de chama cg dic determination of methyl ethyl ketone in urine samples by headspace solid phase micro extraction spme sampling associated to gas chromatography with flame ionization detector gc fid |
| topic | methyl ethyl ketone SPME-GC-FID biological monitoring |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800043 |
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