Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>)
Elongation of very long-chain fatty acids protein 6 (Elovl6) is a crucial enzyme in the synthesis of endogenous fatty acids, which participates in the energy balance and metabolic diseases. The main objective of this study was to explore the molecular characterization of Elovl6 and the regulation of...
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MDPI AG
2020-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/2/264 |
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| author | Yongnan Li Yuning Pang Zengqi Zhao Xiaojun Xiang Kangsen Mai Qinghui Ai |
| author_facet | Yongnan Li Yuning Pang Zengqi Zhao Xiaojun Xiang Kangsen Mai Qinghui Ai |
| author_sort | Yongnan Li |
| collection | DOAJ |
| description | Elongation of very long-chain fatty acids protein 6 (Elovl6) is a crucial enzyme in the synthesis of endogenous fatty acids, which participates in the energy balance and metabolic diseases. The main objective of this study was to explore the molecular characterization of Elovl6 and the regulation of <i>elovl6</i> expression in response to dietary fatty acids and insulin. In the present study, the ORF (open reading frame) of Elovl6 from rainbow trout was cloned and characterized, which showed a high identity (87%) with mammals and other teleost. The results of quantitative PCR showed that the transcriptional levels of <i>elovl6</i> from rainbow trout that were fed diets containing soybean oil (enriched with 18:2n-6, linoleic acid (LA)) or linseed oil (enriched with 18:3n-3, α-linolenic acid (ALA)) were lower than those in the group that were fed diets containing fish oil (enriched with 20:5n-3, eicosapentaenoic acid (EPA) and 22:6n-3, docosahexaenoic acid (DHA)). Correspondingly, mRNA expression of <i>elovl6</i> in hepatocytes treated with DHA was dramatically higher than that in LA and ALA groups. The transcriptional expression of <i>elovl6</i> in hepatocytes treated with insulin was also significantly increased. Moreover, the dual luciferase assay showed the transcription factor CREB1 dramatically up-regulated the promoter activity of <i>elovl6</i>, while FOXO1 significantly down-regulated the <i>elovl6</i> promoter activity in rainbow trout. The differences in transcriptional expression of <i>crbe1</i> and <i>foxo1</i> may contribute to the increase or decrease of <i>elovl6</i> expression in rainbow trout in response to fatty acids or insulin. These findings revealed the molecular characterization of <i>elovl6</i> and the regulation of <i>elovl6</i> expression by CREB1 and FOXO1 in rainbow trout in response to dietary fatty acids or insulin. |
| first_indexed | 2024-04-12T23:25:27Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-12T23:25:27Z |
| publishDate | 2020-02-01 |
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| spelling | doaj.art-e64c227f42f74a888321cacd7e475ed42022-12-22T03:12:26ZengMDPI AGBiomolecules2218-273X2020-02-0110226410.3390/biom10020264biom10020264Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>)Yongnan Li0Yuning Pang1Zengqi Zhao2Xiaojun Xiang3Kangsen Mai4Qinghui Ai5Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaElongation of very long-chain fatty acids protein 6 (Elovl6) is a crucial enzyme in the synthesis of endogenous fatty acids, which participates in the energy balance and metabolic diseases. The main objective of this study was to explore the molecular characterization of Elovl6 and the regulation of <i>elovl6</i> expression in response to dietary fatty acids and insulin. In the present study, the ORF (open reading frame) of Elovl6 from rainbow trout was cloned and characterized, which showed a high identity (87%) with mammals and other teleost. The results of quantitative PCR showed that the transcriptional levels of <i>elovl6</i> from rainbow trout that were fed diets containing soybean oil (enriched with 18:2n-6, linoleic acid (LA)) or linseed oil (enriched with 18:3n-3, α-linolenic acid (ALA)) were lower than those in the group that were fed diets containing fish oil (enriched with 20:5n-3, eicosapentaenoic acid (EPA) and 22:6n-3, docosahexaenoic acid (DHA)). Correspondingly, mRNA expression of <i>elovl6</i> in hepatocytes treated with DHA was dramatically higher than that in LA and ALA groups. The transcriptional expression of <i>elovl6</i> in hepatocytes treated with insulin was also significantly increased. Moreover, the dual luciferase assay showed the transcription factor CREB1 dramatically up-regulated the promoter activity of <i>elovl6</i>, while FOXO1 significantly down-regulated the <i>elovl6</i> promoter activity in rainbow trout. The differences in transcriptional expression of <i>crbe1</i> and <i>foxo1</i> may contribute to the increase or decrease of <i>elovl6</i> expression in rainbow trout in response to fatty acids or insulin. These findings revealed the molecular characterization of <i>elovl6</i> and the regulation of <i>elovl6</i> expression by CREB1 and FOXO1 in rainbow trout in response to dietary fatty acids or insulin.https://www.mdpi.com/2218-273X/10/2/264rainbow trout<i>elovl6</i>fatty acidsinsulin |
| spellingShingle | Yongnan Li Yuning Pang Zengqi Zhao Xiaojun Xiang Kangsen Mai Qinghui Ai Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Biomolecules rainbow trout <i>elovl6</i> fatty acids insulin |
| title | Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full | Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_fullStr | Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full_unstemmed | Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_short | Molecular Characterization, Nutritional and Insulin Regulation of Elovl6 in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_sort | molecular characterization nutritional and insulin regulation of elovl6 in rainbow trout i oncorhynchus mykiss i |
| topic | rainbow trout <i>elovl6</i> fatty acids insulin |
| url | https://www.mdpi.com/2218-273X/10/2/264 |
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