Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe
Introduction: The metabolic balance of amines is closely related to human health. It remains a great challenge to analyze amines with high-throughput and high-coverage.Methods: Polythioester-functionalized magnetic nanoprobes (PMPs) have been prepared under mild conditions and applied in chemoselect...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1103995/full |
| _version_ | 1797961596022030336 |
|---|---|
| author | Yuming Qiu Mo Zhang Zhizhen Lai Renjun Zhang Hongtao Tian Shuai Liu Dan Li Jiang Zhou Zhili Li |
| author_facet | Yuming Qiu Mo Zhang Zhizhen Lai Renjun Zhang Hongtao Tian Shuai Liu Dan Li Jiang Zhou Zhili Li |
| author_sort | Yuming Qiu |
| collection | DOAJ |
| description | Introduction: The metabolic balance of amines is closely related to human health. It remains a great challenge to analyze amines with high-throughput and high-coverage.Methods: Polythioester-functionalized magnetic nanoprobes (PMPs) have been prepared under mild conditions and applied in chemoselective capture of amides. With the introduction of polythioester, PMPs demonstrate remarkably increased capture efficiency, leading to the dramatically improved sensitivity of mass spectrometry detection.Results: The analysis method with PMPs treatment has been applied in rapid detection of more than 100 amines in lung adenocarcinoma cell lines, mouse organ tissues, and 103 human serum samples with high-throughput and high-coverage. Statistical analysis shows that arginine biosynthesis differed between lung adenocarcinoma cell lines.Discussion: Phenylalanine, tyrosine and tryptophan biosynthesis differed between tissues. The combination indicators demonstrate a great diagnostic accuracy for distinguishing between health and lung disease subjects as well as differentiating the patients with benign lung disease and lung cancer. With powerful capture ability, low-cost preparation, and convenient separation, the PMPs demonstrate promising application in the intensive study of metabolic pathways and early diagnosis of disease.high-throughput and high-coverage. Here, polythioester-functionalized magnetic nanoprobes (PMPs) have been prepared under mild conditions and applied in chemoselective capture of amides. With the introduction of polythioester, PMPs demonstrate remarkably increased capture efficiency, leading to the dramatically improved sensitivity of mass spectrometry detection. The analysis method with PMPs treatment has been applied in rapid detection of more than 100 amines in lung adenocarcinoma cell lines, mouse organ tissues, and 103 human serum samples with high-throughput and high-coverage. Statistical analysis shows that arginine biosynthesis differed between lung adenocarcinoma cell lines. Phenylalanine, tyrosine and tryptophan biosynthesis differed between tissues. The combination indicators demonstrate a great diagnostic accuracy for distinguishing between health and lung disease subjects as well as differentiating the patients with benign lung disease and lung cancer. With powerful capture ability, low-cost preparation, and convenient separation, the PMPs demonstrate promising application in the intensive study of metabolic pathways and early diagnosis of disease. |
| first_indexed | 2024-04-11T01:00:20Z |
| format | Article |
| id | doaj.art-e64d63f3635c434bb26b2ef6944b41e2 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T01:00:20Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-e64d63f3635c434bb26b2ef6944b41e22023-01-04T16:56:38ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.11039951103995Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobeYuming Qiu0Mo Zhang1Zhizhen Lai2Renjun Zhang3Hongtao Tian4Shuai Liu5Dan Li6Jiang Zhou7Zhili Li8Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaAnalytical Instrumentation Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaIntroduction: The metabolic balance of amines is closely related to human health. It remains a great challenge to analyze amines with high-throughput and high-coverage.Methods: Polythioester-functionalized magnetic nanoprobes (PMPs) have been prepared under mild conditions and applied in chemoselective capture of amides. With the introduction of polythioester, PMPs demonstrate remarkably increased capture efficiency, leading to the dramatically improved sensitivity of mass spectrometry detection.Results: The analysis method with PMPs treatment has been applied in rapid detection of more than 100 amines in lung adenocarcinoma cell lines, mouse organ tissues, and 103 human serum samples with high-throughput and high-coverage. Statistical analysis shows that arginine biosynthesis differed between lung adenocarcinoma cell lines.Discussion: Phenylalanine, tyrosine and tryptophan biosynthesis differed between tissues. The combination indicators demonstrate a great diagnostic accuracy for distinguishing between health and lung disease subjects as well as differentiating the patients with benign lung disease and lung cancer. With powerful capture ability, low-cost preparation, and convenient separation, the PMPs demonstrate promising application in the intensive study of metabolic pathways and early diagnosis of disease.high-throughput and high-coverage. Here, polythioester-functionalized magnetic nanoprobes (PMPs) have been prepared under mild conditions and applied in chemoselective capture of amides. With the introduction of polythioester, PMPs demonstrate remarkably increased capture efficiency, leading to the dramatically improved sensitivity of mass spectrometry detection. The analysis method with PMPs treatment has been applied in rapid detection of more than 100 amines in lung adenocarcinoma cell lines, mouse organ tissues, and 103 human serum samples with high-throughput and high-coverage. Statistical analysis shows that arginine biosynthesis differed between lung adenocarcinoma cell lines. Phenylalanine, tyrosine and tryptophan biosynthesis differed between tissues. The combination indicators demonstrate a great diagnostic accuracy for distinguishing between health and lung disease subjects as well as differentiating the patients with benign lung disease and lung cancer. With powerful capture ability, low-cost preparation, and convenient separation, the PMPs demonstrate promising application in the intensive study of metabolic pathways and early diagnosis of disease.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1103995/fullmagnetic particlepolythioesterchemoselective captureaminebiomarker |
| spellingShingle | Yuming Qiu Mo Zhang Zhizhen Lai Renjun Zhang Hongtao Tian Shuai Liu Dan Li Jiang Zhou Zhili Li Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe Frontiers in Bioengineering and Biotechnology magnetic particle polythioester chemoselective capture amine biomarker |
| title | Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe |
| title_full | Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe |
| title_fullStr | Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe |
| title_full_unstemmed | Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe |
| title_short | Profiling of amines in biological samples using polythioester-functionalized magnetic nanoprobe |
| title_sort | profiling of amines in biological samples using polythioester functionalized magnetic nanoprobe |
| topic | magnetic particle polythioester chemoselective capture amine biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1103995/full |
| work_keys_str_mv | AT yumingqiu profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT mozhang profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT zhizhenlai profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT renjunzhang profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT hongtaotian profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT shuailiu profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT danli profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT jiangzhou profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe AT zhilili profilingofaminesinbiologicalsamplesusingpolythioesterfunctionalizedmagneticnanoprobe |