Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice
Introduction: The GABAergic system of the brain plays a key role in morphine tolerance and sensitization. As isoniazid is a modulator of the GABAergic system, the present study aims to understand whether isoniazid can influence the induction of tolerance and sensitization to the rewarding effects of...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2020-07-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/article-1-1557-en.html |
| _version_ | 1797283125235023872 |
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| author | Amir Abbas Barzegari Kamran Shahabi |
| author_facet | Amir Abbas Barzegari Kamran Shahabi |
| author_sort | Amir Abbas Barzegari |
| collection | DOAJ |
| description | Introduction: The GABAergic system of the brain plays a key role in morphine tolerance and sensitization. As isoniazid is a modulator of the GABAergic system, the present study aims to understand whether isoniazid can influence the induction of tolerance and sensitization to the rewarding effects of morphine.
Methods: The rewarding effects of morphine and isoniazid were assessed using a Conditioned Place Preference (CPP) procedure in female mice. Tolerance to the rewarding effects of morphine was induced with high-dose morphine (25 mg/kg, SC), twice a day, for four days. Also, the sensitization was induced with an effective dose of morphine (5 mg/kg, SC), once a day, for three days. During the induction of tolerance or sensitization, the different groups of mice received saline or isoniazid (25, 50, and 75 mg/kg, IP) one hour before each morphine injection.
Results: Morphine (0.5-10 mg/kg, SC) produced a significant CPP, but isoniazid (25, 50, and 75 mg/kg, IP) did not induce place preference or place aversion in mice. Although an effective dose of morphine (5 mg/kg, SC) did not induce CPP in morphine tolerated mice, an ineffective dose (0.5 mg/kg, SC) could produce a significant CPP in morphine-sensitized animals. The administration of isoniazid before morphine (on the days of tolerance or sensitization induction) inhibited the development of tolerance or sensitization to the rewarding effect of morphine in the CPP paradigm.
Conclusion: Isoniazid can be a useful drug for the prevention of tolerance and sensitization to the rewarding effects of morphine. |
| first_indexed | 2024-03-07T17:25:41Z |
| format | Article |
| id | doaj.art-e65311836e3f46cdae72485e9b341bb3 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:25:41Z |
| publishDate | 2020-07-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-e65311836e3f46cdae72485e9b341bb32024-03-02T19:16:34ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422020-07-01114481490Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in MiceAmir Abbas Barzegari0Kamran Shahabi1 Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran. Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran. Introduction: The GABAergic system of the brain plays a key role in morphine tolerance and sensitization. As isoniazid is a modulator of the GABAergic system, the present study aims to understand whether isoniazid can influence the induction of tolerance and sensitization to the rewarding effects of morphine. Methods: The rewarding effects of morphine and isoniazid were assessed using a Conditioned Place Preference (CPP) procedure in female mice. Tolerance to the rewarding effects of morphine was induced with high-dose morphine (25 mg/kg, SC), twice a day, for four days. Also, the sensitization was induced with an effective dose of morphine (5 mg/kg, SC), once a day, for three days. During the induction of tolerance or sensitization, the different groups of mice received saline or isoniazid (25, 50, and 75 mg/kg, IP) one hour before each morphine injection. Results: Morphine (0.5-10 mg/kg, SC) produced a significant CPP, but isoniazid (25, 50, and 75 mg/kg, IP) did not induce place preference or place aversion in mice. Although an effective dose of morphine (5 mg/kg, SC) did not induce CPP in morphine tolerated mice, an ineffective dose (0.5 mg/kg, SC) could produce a significant CPP in morphine-sensitized animals. The administration of isoniazid before morphine (on the days of tolerance or sensitization induction) inhibited the development of tolerance or sensitization to the rewarding effect of morphine in the CPP paradigm. Conclusion: Isoniazid can be a useful drug for the prevention of tolerance and sensitization to the rewarding effects of morphine.http://bcn.iums.ac.ir/article-1-1557-en.htmlisoniazidmorphineconditioned place preferencetolerancesensitizationreward |
| spellingShingle | Amir Abbas Barzegari Kamran Shahabi Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice Basic and Clinical Neuroscience isoniazid morphine conditioned place preference tolerance sensitization reward |
| title | Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice |
| title_full | Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice |
| title_fullStr | Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice |
| title_full_unstemmed | Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice |
| title_short | Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice |
| title_sort | effects of isoniazid on tolerance and sensitization to the rewarding properties of morphine a conditioned place preference procedure investigation in mice |
| topic | isoniazid morphine conditioned place preference tolerance sensitization reward |
| url | http://bcn.iums.ac.ir/article-1-1557-en.html |
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