Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons.
GABAergic interneurons tend to diversify into similar classes across telencephalic regions. However, it remains unclear whether the electrophysiological and molecular properties commonly used to define these classes are discriminant in the hilus of the dentate gyrus. Here, using patch-clamp combined...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0270981 |
| _version_ | 1818162255066300416 |
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| author | Quentin Perrenoud Clémence Leclerc Hélène Geoffroy Tania Vitalis Kevin Richetin Claire Rampon Thierry Gallopin |
| author_facet | Quentin Perrenoud Clémence Leclerc Hélène Geoffroy Tania Vitalis Kevin Richetin Claire Rampon Thierry Gallopin |
| author_sort | Quentin Perrenoud |
| collection | DOAJ |
| description | GABAergic interneurons tend to diversify into similar classes across telencephalic regions. However, it remains unclear whether the electrophysiological and molecular properties commonly used to define these classes are discriminant in the hilus of the dentate gyrus. Here, using patch-clamp combined with single cell RT-PCR, we compare the relevance of commonly used electrophysiological and molecular features for the clustering of GABAergic interneurons sampled from the mouse hilus and primary sensory cortex. While unsupervised clustering groups cortical interneurons into well-established classes, it fails to provide a convincing partition of hilar interneurons. Statistical analysis based on resampling indicates that hilar and cortical GABAergic interneurons share limited homology. While our results do not invalidate the use of classical molecular marker in the hilus, they indicate that classes of hilar interneurons defined by the expression of molecular markers do not exhibit strongly discriminating electrophysiological properties. |
| first_indexed | 2024-12-11T16:30:45Z |
| format | Article |
| id | doaj.art-e656e7ccc29c4e4c9aecb84449d4ca20 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T16:30:45Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e656e7ccc29c4e4c9aecb84449d4ca202022-12-22T00:58:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01177e027098110.1371/journal.pone.0270981Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons.Quentin PerrenoudClémence LeclercHélène GeoffroyTania VitalisKevin RichetinClaire RamponThierry GallopinGABAergic interneurons tend to diversify into similar classes across telencephalic regions. However, it remains unclear whether the electrophysiological and molecular properties commonly used to define these classes are discriminant in the hilus of the dentate gyrus. Here, using patch-clamp combined with single cell RT-PCR, we compare the relevance of commonly used electrophysiological and molecular features for the clustering of GABAergic interneurons sampled from the mouse hilus and primary sensory cortex. While unsupervised clustering groups cortical interneurons into well-established classes, it fails to provide a convincing partition of hilar interneurons. Statistical analysis based on resampling indicates that hilar and cortical GABAergic interneurons share limited homology. While our results do not invalidate the use of classical molecular marker in the hilus, they indicate that classes of hilar interneurons defined by the expression of molecular markers do not exhibit strongly discriminating electrophysiological properties.https://doi.org/10.1371/journal.pone.0270981 |
| spellingShingle | Quentin Perrenoud Clémence Leclerc Hélène Geoffroy Tania Vitalis Kevin Richetin Claire Rampon Thierry Gallopin Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons. PLoS ONE |
| title | Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons. |
| title_full | Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons. |
| title_fullStr | Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons. |
| title_full_unstemmed | Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons. |
| title_short | Molecular and electrophysiological features of GABAergic neurons in the dentate gyrus reveal limited homology with cortical interneurons. |
| title_sort | molecular and electrophysiological features of gabaergic neurons in the dentate gyrus reveal limited homology with cortical interneurons |
| url | https://doi.org/10.1371/journal.pone.0270981 |
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