The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats

Background & Aims: Idiopathic pulmonary fibrosis (IPF) is a chronic diffuse interstitial lung disease, which has symptoms such as coughing and shortness of breath. The average survival duration in these patients is estimated as 3 years after the onset of symptoms. Today, the therapeutic approach...

Full description

Bibliographic Details
Main Authors: Mitra Samareh-Fekri, Hamid-Reza Poursalehi, Ali Mandegary, Fariba Sharififar, Rahil Mahmoudi, Atefe Izadi, Mohamad-Hadi Nematolahi, Nasrollah Jamshidi, Fatemeh Pourgholamhossein, Mohammad-Reza Lashkarizadeh
Format: Article
Language:English
Published: Kerman University of Medical Sciences 2015-12-01
Series:Journal of Kerman University of Medical Sciences
Subjects:
Online Access:https://jkmu.kmu.ac.ir/article_16075_2140c742ca5a01b02191f4132f7bd636.pdf
_version_ 1827911882179084288
author Mitra Samareh-Fekri
Hamid-Reza Poursalehi
Ali Mandegary
Fariba Sharififar
Rahil Mahmoudi
Atefe Izadi
Mohamad-Hadi Nematolahi
Nasrollah Jamshidi
Fatemeh Pourgholamhossein
Mohammad-Reza Lashkarizadeh
author_facet Mitra Samareh-Fekri
Hamid-Reza Poursalehi
Ali Mandegary
Fariba Sharififar
Rahil Mahmoudi
Atefe Izadi
Mohamad-Hadi Nematolahi
Nasrollah Jamshidi
Fatemeh Pourgholamhossein
Mohammad-Reza Lashkarizadeh
author_sort Mitra Samareh-Fekri
collection DOAJ
description Background & Aims: Idiopathic pulmonary fibrosis (IPF) is a chronic diffuse interstitial lung disease, which has symptoms such as coughing and shortness of breath. The average survival duration in these patients is estimated as 3 years after the onset of symptoms. Today, the therapeutic approach used in IPF cases is the usage of chemical or herbal antioxidants in order to eliminate free radicals and prevent inflammation and fibrosis. Considering the antioxidant properties of fennel, this research was conducted to investigate the effect of the methanol extracted of fennel on bleomycin-induced pulmonary fibrosis in rats. Methods: To induce pulmonary fibrosis in rats, intratracheal administration of 15 mg bleomycin was conducted in 52 male rates. Subsequently, the rats were injected intraperitoneally with 500 mg/(kg body weight) of methanol extract of fennel 14-day and 28-day periods. The samples were studied in terms of inflammation, fibrosis, hydroxyproline concentration, lipid peroxidation, and the catalase activity of lung tissue. The obtained results were analyzed using SPSS software. Results: The degree of inflammation, fibrosis, hydroxyproline and lipid peroxidation concentration in lung tissue in the fennel methanol extract group had significantly decreased compared to other groups. In addition, a significant increase was observed in tissue catalase concentration in this group in comparison to other groups on days 14 and 28. Conclusion: The obtained results illustrated that the methanol extract of fennel may be efficient in the prevention and treatment of pulmonary fibrosis due to its antioxidant properties. Accordingly, it can be suggested for the prevention and treatment of pulmonary fibrosis instead of corticosteroids and immunosuppressant drugs due to their many side effects and most patients’ preference of herbal medicines.
first_indexed 2024-03-13T02:08:36Z
format Article
id doaj.art-e65af0920f834e668a4a1c97e97cbc80
institution Directory Open Access Journal
issn 2008-2843
language English
last_indexed 2024-03-13T02:08:36Z
publishDate 2015-12-01
publisher Kerman University of Medical Sciences
record_format Article
series Journal of Kerman University of Medical Sciences
spelling doaj.art-e65af0920f834e668a4a1c97e97cbc802023-07-01T05:24:45ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432015-12-0122547048316075The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in RatsMitra Samareh-Fekri0Hamid-Reza Poursalehi1Ali Mandegary2Fariba Sharififar3Rahil Mahmoudi4Atefe Izadi5Mohamad-Hadi Nematolahi6Nasrollah Jamshidi7Fatemeh Pourgholamhossein8Mohammad-Reza Lashkarizadeh9Assistant Professor, Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranCardiovascular Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranGasteroenterology & Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranAssistant Professor, Department of Pharmacognosy and Herbal and Traditional Medicines Research Center, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranSchool of Pharmacy, Kerman University of Medical Sciences, Kerman, IranSchool of Pharmacy, Kerman University of Medical Sciences, Kerman, IranHerbal and Traditional Medicines Research Center, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranAfzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranAssociate Professor, Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranBackground & Aims: Idiopathic pulmonary fibrosis (IPF) is a chronic diffuse interstitial lung disease, which has symptoms such as coughing and shortness of breath. The average survival duration in these patients is estimated as 3 years after the onset of symptoms. Today, the therapeutic approach used in IPF cases is the usage of chemical or herbal antioxidants in order to eliminate free radicals and prevent inflammation and fibrosis. Considering the antioxidant properties of fennel, this research was conducted to investigate the effect of the methanol extracted of fennel on bleomycin-induced pulmonary fibrosis in rats. Methods: To induce pulmonary fibrosis in rats, intratracheal administration of 15 mg bleomycin was conducted in 52 male rates. Subsequently, the rats were injected intraperitoneally with 500 mg/(kg body weight) of methanol extract of fennel 14-day and 28-day periods. The samples were studied in terms of inflammation, fibrosis, hydroxyproline concentration, lipid peroxidation, and the catalase activity of lung tissue. The obtained results were analyzed using SPSS software. Results: The degree of inflammation, fibrosis, hydroxyproline and lipid peroxidation concentration in lung tissue in the fennel methanol extract group had significantly decreased compared to other groups. In addition, a significant increase was observed in tissue catalase concentration in this group in comparison to other groups on days 14 and 28. Conclusion: The obtained results illustrated that the methanol extract of fennel may be efficient in the prevention and treatment of pulmonary fibrosis due to its antioxidant properties. Accordingly, it can be suggested for the prevention and treatment of pulmonary fibrosis instead of corticosteroids and immunosuppressant drugs due to their many side effects and most patients’ preference of herbal medicines.https://jkmu.kmu.ac.ir/article_16075_2140c742ca5a01b02191f4132f7bd636.pdffennelpulmonary fibrosisbleomycin
spellingShingle Mitra Samareh-Fekri
Hamid-Reza Poursalehi
Ali Mandegary
Fariba Sharififar
Rahil Mahmoudi
Atefe Izadi
Mohamad-Hadi Nematolahi
Nasrollah Jamshidi
Fatemeh Pourgholamhossein
Mohammad-Reza Lashkarizadeh
The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats
Journal of Kerman University of Medical Sciences
fennel
pulmonary fibrosis
bleomycin
title The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats
title_full The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats
title_fullStr The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats
title_full_unstemmed The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats
title_short The Effect of Methanol Extract of Fennel on Bleomycin-Induced Pulmonary Fibrosis in Rats
title_sort effect of methanol extract of fennel on bleomycin induced pulmonary fibrosis in rats
topic fennel
pulmonary fibrosis
bleomycin
url https://jkmu.kmu.ac.ir/article_16075_2140c742ca5a01b02191f4132f7bd636.pdf
work_keys_str_mv AT mitrasamarehfekri theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT hamidrezapoursalehi theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT alimandegary theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT faribasharififar theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT rahilmahmoudi theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT atefeizadi theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT mohamadhadinematolahi theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT nasrollahjamshidi theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT fatemehpourgholamhossein theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT mohammadrezalashkarizadeh theeffectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT mitrasamarehfekri effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT hamidrezapoursalehi effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT alimandegary effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT faribasharififar effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT rahilmahmoudi effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT atefeizadi effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT mohamadhadinematolahi effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT nasrollahjamshidi effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT fatemehpourgholamhossein effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats
AT mohammadrezalashkarizadeh effectofmethanolextractoffennelonbleomycininducedpulmonaryfibrosisinrats